A quantitative detection of Cardiotrophin‐1 in chronic heart failure by chemiluminescence immunoassay
| العنوان: | A quantitative detection of Cardiotrophin‐1 in chronic heart failure by chemiluminescence immunoassay |
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| المؤلفون: | Danhua Wang, Zhihua Tao, Weiwei Liu, Pan Yu, Ying Ping, Xuchu Wang, Yibei Dai |
| المصدر: | Journal of Clinical Laboratory Analysis |
| بيانات النشر: | John Wiley and Sons Inc., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Cardiotrophin‐1, Clinical Biochemistry, chemistry.chemical_compound, 0302 clinical medicine, Limit of Detection, Immunology and Allergy, Research Articles, Aged, 80 and over, Immunoassay, biology, Hematology, Middle Aged, chronic heart failure, Medical Laboratory Technology, chemiluminescence immunoassay, 030220 oncology & carcinogenesis, Calibration, Cytokines, Female, quantitative detection, Antibody, Research Article, Microbiology (medical), Streptavidin, Adult, Cardiotrophin 1, Coefficient of variation, 03 medical and health sciences, Young Adult, paramagnetic particles, medicine, Humans, Aged, Detection limit, Heart Failure, Reproducibility, Chromatography, Biochemistry (medical), Public Health, Environmental and Occupational Health, medicine.disease, 030104 developmental biology, chemistry, Linear range, Heart failure, Case-Control Studies, Chronic Disease, Luminescent Measurements, biology.protein |
| الوصف: | Background Cardiotrophin‐1 (CT‐1) is a cytokine that could induce cardiomyocytes hypertrophy and dysfunction. Plasma CT‐1 might serve as a cardiac biomarker both in diagnosis, staging, and prognostic assessment of heart failure. Methods In this study, a one‐step paramagnetic particles‐based chemiluminescence immunoassay (MPs‐CILA) for rapid and sensitive detection of plasma CT‐1 was established. Plasma samples were directly incubated with biotin‐labeled anti‐CT‐1 antibody (bio‐Ab) and acridine ester labeled anti‐CT‐1 antibody (AE‐Ab) to form sandwiched complex. The sandwiched CT‐1 was then captured by streptavidin modified paramagnetic particles (MPs‐SA) for rapid separation and signal generation. Results The proposed MPs‐CLIA presents a laudable linear relationship ranging from 7.8 pg/mL to 200 ng/mL with a detection limit of 1.0 pg/mL. The recoveries of spiked human plasma samples at low (10pg/mL), medium (100 pg/mL), and high (800 pg/mL) levels of CT‐1 were 96%, 104%, and 110% respectively. The intra‐analysis coefficient variation (CVs) of the 3 samples was 8.92%, 6.69%, and 3.54%, respectively. And the inter‐analysis coefficient variation (CVs) was 9.25%, 10.9%, and 4.3%, respectively. These results strongly indicate high sensitivity, wide linear range, acceptable precision, and applicable reproducibility of the proposed method to detect plasma level of CT‐1. Finally, Plasma CT‐1 from 140 subjects with or without chronic heart failure was analyzed to assess the clinical application of MPs‐CILA. Conclusions Noteworthily, the MPs‐CLIA method is highly automated such that it is suitable for high‐throughput detection of CT‐1 in clinical inspection. A one‐step paramagnetic particles‐based chemiluminescence immunoassay (MPs‐CILA) for rapid and sensitive detection of plasma CT‐1 was established, and plasma CT‐1 from 140 subjects with or without chronic heart failure was analyzed to assess the clinical application of MPs‐CILA. |
| اللغة: | English |
| تدمد: | 1098-2825 0887-8013 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72b477ab7366f04abb42dc944e3c0e3eTest http://europepmc.org/articles/PMC8059751Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....72b477ab7366f04abb42dc944e3c0e3e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10982825 08878013 |
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