دورية أكاديمية
Detection of wildtype Merkel cell polyomavirus genomic sequence and VP1 transcription in a subset of Merkel cell carcinoma.
| العنوان: | Detection of wildtype Merkel cell polyomavirus genomic sequence and VP1 transcription in a subset of Merkel cell carcinoma. |
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| المؤلفون: | Kervarrec, Thibault, Appenzeller, Silke, Tallet, Anne, Jullie, Marie-Laure, Sohier, Pierre, Guillonneau, Francois, Rütten, Arno, Berthon, Patricia, Le Corre, Yannick, Hainaut-Wierzbicka, Ewa, Blom, Astrid, Beneton, Nathalie, Bens, Guido, Nardin, Charline, Aubin, Francois, Dinulescu, Monica, Visée, Sebastien, Herfs, Michael, Touzé, Antoine, Guyétant, Serge, Samimi, Mahtab, Houben, Roland, Schrama, David |
| المصدر: | Histopathology (2024-01) |
| بيانات النشر: | John Wiley and Sons Inc |
| سنة النشر: | 2024 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | Merkel cell carcinoma, VP1, polyomavirus, replication, trichoblastoma, Pathology and Forensic Medicine, Histology, General Medicine, Human health sciences, Oncology, Sciences de la santé humaine, Oncologie |
| الوصف: | peer reviewed ; [en] AIMS: Merkel cell carcinoma (MCC) is frequently caused by the Merkel cell polyomavirus (MCPyV). Characteristic for these virus-positive (VP) MCC is MCPyV integration into the host genome and truncation of the viral oncogene Large T antigen (LT), with full-length LT expression considered as incompatible with MCC growth. Genetic analysis of a VP-MCC/trichoblastoma combined tumour demonstrated that virus-driven MCC can arise from an epithelial cell. Here we describe two further cases of VP-MCC combined with an adnexal tumour, i.e. one trichoblastoma and one poroma. METHODS AND RESULTS: Whole-genome sequencing of MCC/trichoblastoma again provided evidence of a trichoblastoma-derived MCC. Although an MCC-typical LT-truncating mutation was detected, we could not determine an integration site and we additionally detected a wildtype sequence encoding full-length LT. Similarly, Sanger sequencing of the combined MCC/poroma revealed coding sequences for both truncated and full-length LT. Moreover, in situ RNA hybridization demonstrated expression of a late region mRNA encoding the viral capsid protein VP1 in both combined as well as in a few cases of pure MCC. CONCLUSION: The data presented here suggest the presence of wildtype MCPyV genomes and VP1 transcription in a subset of MCC. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0309-0167 1365-2559 |
| العلاقة: | urn:issn:0309-0167; urn:issn:1365-2559; https://orbi.uliege.be/handle/2268/308089Test; info:hdl:2268/308089; scopus-id:2-s2.0-85173999040; info:pmid:37830288 |
| DOI: | 10.1111/his.15068 |
| الإتاحة: | https://doi.org/10.1111/his.15068Test https://orbi.uliege.be/handle/2268/308089Test |
| حقوق: | restricted access ; http://purl.org/coar/access_right/c_16ecTest ; info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.52A8BE6A |
| قاعدة البيانات: | BASE |
| تدمد: | 03090167 13652559 |
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| DOI: | 10.1111/his.15068 |