دورية أكاديمية
Multisystem screening reveals SARS-CoV-2 in neurons of the myenteric plexus and in megakaryocytes.
| العنوان: | Multisystem screening reveals SARS-CoV-2 in neurons of the myenteric plexus and in megakaryocytes. |
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| المؤلفون: | Gray-Rodriguez, Sandra, Jensen, Melanie P, Otero-Jimenez, Maria, Hanley, Brian, Swann, Olivia C, Ward, Patrick A, Salguero, Francisco J, Querido, Nadira, Farkas, Ildiko, Velentza-Almpani, Elisavet, Weir, Justin, Barclay, Wendy S, Carroll, Miles W, Jaunmuktane, Zane, Brandner, Sebastian, Pohl, Ute, Allinson, Kieren, Thom, Maria, Troakes, Claire, Al-Sarraj, Safa, Sastre, Magdalena, Gveric, Djordje, Gentleman, Steve, Roufosse, Candice, Osborn, Michael, Alegre-Abarrategui, Javier |
| بيانات النشر: | John Wiley And Sons |
| سنة النشر: | 2022 |
| المجموعة: | Heart of England: HEFT Repository |
| مصطلحات موضوعية: | QW Microbiology. Immunology, QZ Pathology. Oncology, WC Communicabable diseases, WL Nervous system. Neurology |
| الوصف: | SARS-CoV-2, the causative agent of COVID-19, typically manifests as a respiratory illness although extrapulmonary involvement, such as in the gastrointestinal tract and nervous system, as well as frequent thrombotic events, are increasingly recognised. How this maps onto SARS-CoV-2 organ tropism at the histological level, however, remains unclear. Here, we perform a comprehensive validation of a monoclonal antibody against the SARS-CoV-2 nucleocapsid protein (NP) followed by systematic multisystem organ immunohistochemistry analysis of the viral cellular tropism in tissue from 36 patients, 16 post-mortem cases and 16 biopsies with polymerase chain reaction (PCR)-confirmed SARS-CoV-2 status from the peaks of the pandemic in 2020 and four pre-COVID post-mortem controls. SARS-CoV-2 anti-NP staining in the post-mortem cases revealed broad multiorgan involvement of the respiratory, digestive, haematopoietic, genitourinary and nervous systems, with a typical pattern of staining characterised by punctate paranuclear and apical cytoplasmic labelling. The average time from symptom onset to time of death was shorter in positively versus negatively stained post-mortem cases (mean = 10.3 days versus mean = 20.3 days, p = 0.0416, with no cases showing definitive staining if the interval exceeded 15 days). One striking finding was the widespread presence of SARS-CoV-2 NP in neurons of the myenteric plexus, a site of high ACE-2 expression, the entry receptor for SARS-CoV-2, and one of the earliest affected cells in Parkinson's disease. In the bone marrow, we observed viral SARS-CoV-2 NP within megakaryocytes, key cells in platelet production and thrombus formation. In 15 tracheal biopsies performed in patients requiring ventilation, there was a near complete concordance between immunohistochemistry and PCR swab results. Going forward, our findings have relevance to correlating clinical symptoms to the organ tropism of SARS-CoV-2 in contemporary cases as well as providing insights into potential long-term complications of ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | Gray-Rodriguez, Sandra, Jensen, Melanie P, Otero-Jimenez, Maria, Hanley, Brian, Swann, Olivia C, Ward, Patrick A, Salguero, Francisco J, Querido, Nadira, Farkas, Ildiko, Velentza-Almpani, Elisavet, Weir, Justin, Barclay, Wendy S, Carroll, Miles W, Jaunmuktane, Zane, Brandner, Sebastian, Pohl, Ute, Allinson, Kieren, Thom, Maria, Troakes, Claire, Al-Sarraj, Safa, Sastre, Magdalena, Gveric, Djordje, Gentleman, Steve, Roufosse, Candice, Osborn, Michael and Alegre-Abarrategui, Javier (2022) Multisystem screening reveals SARS-CoV-2 in neurons of the myenteric plexus and in megakaryocytes. The Journal of pathology. ISSN 1096-9896. This article is available to all UHB staff and students login using a UHB Athens Account. Register for Athens here - https://openathens.nice.org.ukTest/ |
| DOI: | 10.1002/path.5878 |
| الإتاحة: | https://doi.org/10.1002/path.5878Test http://www.repository.uhblibrary.co.uk/id/eprint/5165Test/ https://onlinelibrary.wiley.com/journal/10969896Test |
| رقم الانضمام: | edsbas.98FBF9D9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/path.5878 |
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