دورية أكاديمية
Mass spectrometric analysis of the glycosphingolipid-derived glycans from miniature pig endothelial cells and islets: identification of NeuGc epitope in pig islets
| العنوان: | Mass spectrometric analysis of the glycosphingolipid-derived glycans from miniature pig endothelial cells and islets: identification of NeuGc epitope in pig islets |
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| المؤلفون: | Kim, Yun-Gon, Harvey, David J., Yang, Yung-Hun, Park, Chung-Gyu, Kim, Byung-Gee |
| المساهمون: | 김윤곤, 양영훈, 박정규, 김병지 |
| بيانات النشر: | JOHN WILEY & SONS LTD |
| سنة النشر: | 2009 |
| المجموعة: | Seoul National University: S-Space |
| مصطلحات موضوعية: | xenotransplantation, mass spectrometry, N-glycolylneuraminic acid, pig islet cells, pig endothelial cells, glycosphingolipid |
| الوصف: | Glycosphingolipid (GSL) is a major component of the plasma membrane in eukaryotic cells that is involved directly in a variety of immunological events via cell-to-cell or cell-to-protein interactions. In this study, qualitative and quantitative analyses of GSL-derived glycans on endothelial cells and islets from a miniature pig were performed and their glycosylation patterns were compared. A total of 60 and 47 sialylated and neutral GSL-derived glycans from the endothelial cells and islets, respectively, were characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and collision-induced fragmentation using positive-ion electrospray ionization (ESI) ion-trap tandem mass spectrometry (MS/MS). In accordance with previous immunohistochemistry studies, the alpha-Gal-terminated GSL was not detected but NeuGc-terminated GSLs were newly detected from miniature pig islets. In addition, the neutral GSL-derived glycans were relatively quantified by derivatization with carboxymethyl trimethylammonium hydrazide (so called Girard`s T reagent) and MALDI-TOF MS. The structural information of the GSL-derived glycans from pig endothelial cells and islets suggests that special attention should be paid to all types of glycoconjugates expressed on pig tissues or cells for successful clinical xenotransplantation. Copyright (C) 2009 John Wiley & Sons, Ltd. ; Kim YG, 2009, J MASS SPECTROM, V44, P1087, DOI 10.1002/jms.1587 ; Gil GC, 2008, ANAL BIOCHEM, V379, P45, DOI 10.1016/j.ab.2008.04.039 ; Kim YG, 2008, PROTEOMICS, V8, P2596, DOI 10.1002/pmic.200700972 ; Jang KS, 2008, BIOTECHNOL BIOPROC E, V13, P445, DOI 10.1007/s12257-008-0141-1 ; Li YS, 2008, GLYCOBIOLOGY, V18, P166, DOI 10.1093/glycob/cwm127 ; Kang P, 2007, ANAL CHEM, V79, P6064, DOI 10.1021/ac062098r ; Parry S, 2007, GLYCOBIOLOGY, V17, P646, DOI 10.1093/glycob/cwm024 ; Kim JH, 2007, XENOTRANSPLANTATION, V14, P60, DOI 10.1111/j.1399-3089.2006.00364.x ; Milland J, 2006, J IMMUNOL, V176, P2448 ; Kim YG, 2006, PROTEOMICS, V6, ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1076-5174 |
| العلاقة: | JOURNAL OF MASS SPECTROMETRY; Vol.44 10; 1489-1499; http://hdl.handle.net/10371/76940Test |
| DOI: | 10.1002/jms.1638 |
| الإتاحة: | https://doi.org/10.1002/jms.1638Test http://hdl.handle.net/10371/76940Test |
| رقم الانضمام: | edsbas.2E339C07 |
| قاعدة البيانات: | BASE |
| تدمد: | 10765174 |
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| DOI: | 10.1002/jms.1638 |