دورية أكاديمية
Evaluation of Alcaligenes eutrophus cells as an NADH regenerating catalyst in organic-aqueous two-phase system
| العنوان: | Evaluation of Alcaligenes eutrophus cells as an NADH regenerating catalyst in organic-aqueous two-phase system |
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| المؤلفون: | Andersson, Mats, Holmberg, Hans, Adlercreutz, Patrick |
| المصدر: | Biotechnology and Bioengineering; 57(1), pp 79-86 (1998) ; ISSN: 0006-3592 |
| بيانات النشر: | John Wiley & Sons Inc. |
| سنة النشر: | 1998 |
| المجموعة: | Lund University Publications (LUP) |
| مصطلحات موضوعية: | Biocatalysis and Enzyme Technology, Alcaligenes eutrophus, HLADH, Hydrogenase, NADH regeneration, Organic solvent |
| الوصف: | A soluble NAD-dependent hydrogenase contained in Alcaligenes eutrophus was evaluated as a coenzyme regenerating catalyst in an organic-aqueous two- phase (predominantly organic) system. The horse-liver alcohol-dehydrogenase (HLADH) catalyzed reduction of cyclohexanone to cyclohexanol was used as a model reaction. The impact of different solvents (selected to span a large variety of principal properties) on the stability and activity of the HLADH, using substrate-driven regeneration, was studied. Solvents suitable for the HLADH were then selected for an evaluation of the hydrogenase-driven coenzyme regeneration. Hydrophobic solvents such as heptane, toluene; and 1,1,1- trichloroethane were found to be suitable for the coupled reactions catalyzed by HLADH and hydrogenase. Nonimmobilized cells, permeabilized with cetyl- trimethyl-ammonium bromide, were the most efficient preparation for the regeneration of NADH. The use of this preparation in heptane (10% water) was optimized with respect to the yield obtained in the HLADH-catalyzed reduction of cyclohexanone. Using the optimized conditions, yields of 99% cyclohexanol were obtained. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-03-248470-2 0-03-248470-4 |
| العلاقة: | https://lup.lub.lu.se/record/36251d17-f910-4cc2-9caa-4cc12d267875Test; http://dx.doi.org/10.1002Test/(SICI)1097-0290(19980105)57:13.0.CO;2-3; scopus:0032484704; pmid:10099181 |
| DOI: | 10.1002/(SICI)1097-0290(19980105)57:1<79::AID-BIT10>3.0.CO;2-3 |
| الإتاحة: | https://doi.org/10.1002Test/(SICI)1097-0290(19980105)57:1<79::AID-BIT10>3.0.CO;2-3 https://lup.lub.lu.se/record/36251d17-f910-4cc2-9caa-4cc12d267875Test |
| رقم الانضمام: | edsbas.3DE45D80 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780032484702 0032484704 |
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| DOI: | 10.1002/(SICI)1097-0290(19980105)57:1<79::AID-BIT10>3.0.CO;2-3 |