Bovine peripheral blood MSCs chemotax towards inflammation and embryo implantation stimuli
| العنوان: | Bovine peripheral blood MSCs chemotax towards inflammation and embryo implantation stimuli |
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| المؤلفون: | Alexandra Calle, Marta Monguió-Tortajada, Miguel Ángel Ramírez, Miguel Ángel Gutiérrez‐Reinoso, Javier Blanco, Francesc E. Borràs, Michela Re, Julio De la Fuente, María Yáñez-Mó |
| المساهمون: | Ministerio de Industria y Competitividad (España), Ministerio de Ciencia e Innovación (España), Ministerio de Transición Ecológica (España), Fundación Biodiversidad |
| المصدر: | Journal of Cellular Physiology r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol instname Digital.CSIC. Repositorio Institucional del CSIC |
| بيانات النشر: | John Wiley & Sons Inc., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, cell migration, Physiology, immunoregulation, medicine.medical_treatment, Clinical Biochemistry, Inflammation, Biology, peripheral blood mesenchymal stem cells, Regenerative Medicine, Regenerative medicine, 03 medical and health sciences, 0302 clinical medicine, Osteogenesis, medicine, Animals, Humans, Cell migration, embryo implantation, Embryo Implantation, Cells, Cultured, Cell Proliferation, Adipogenesis, Chemotaxis, Mesenchymal stem cell, Immunoregulation, Embryo, Cell Differentiation, Mesenchymal Stem Cells, Cell Biology, Embryonic stem cell, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Cytokine, 030220 oncology & carcinogenesis, Maternal-Fetal Relations, Granulocyte colony‐stimulating factor, Leukocytes, Mononuclear, Cattle, Female, Bone marrow, medicine.symptom, granulocyte colony-stimulating factor, Chondrogenesis |
| الوصف: | Mesenchymal stem cells (MSCs) have a great potential in regenerative medicine because of their multipotential and immunoregulatory capacities, while in early pregnancy they could participate in the immunotolerance of the mother towards the embryo. Peripheral blood constitutes an accessible source of MSCs. We successfully isolated peripheral blood MSC (pbMSCs) lines, with or without previous bone marrow mobilization. All pbMSCs lines obtained in both conditions presented classical MSC markers and properties, alkaline phosphatase activity and multipotent capacity to differentiate among adipogenic, osteogenic or chondrogenic lineages, and suppressed the proliferation of T cells. pbMSCs showed migratory capacity without cytokine stimulation while increasing their migration rate in the presence of inflammatory or embryo implantation stimuli. Interestingly, in contrast to MSCs derived from endometrial tissue, three pbMSCs lines also showed increased migration towards the IFN-τ implantation cytokine. Moreover, the secretome produced by an early implantation stage embryonic trophectoderm cell line showed a chemoattractant effect in pbMSCs. Our results suggest that circulating MSCs are present in the peripheral blood under healthy conditions. The fact that both the inflammation and implantation signals induced pbMSCs chemotaxis highlights MSC heterogeneity and suggests that their migratory capacity may differ according to their tissue of origin and would suggest the possible active recruitment of MSCs from bone marrow during pregnancy to repress the immune response to prevent the embryo rejection by the maternal organism. Spanish Ministerio de Economía Industria y Competitividad to Ramírez M. A. (AGL2015‐70140‐R), Spanish Ministerio de Ciencia e Innovación to Ramírez M. A. (PID2019‐107145RB‐I00), Spanish Ministerio para la Transición Ecológica y el Reto Demográfico, through Fundación Biodiversidad to Ramírez M. A. (PRCV00820) and European Union's Horizon |
| تدمد: | 0021-9541 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::521d7002c73abbb93e3d0174e5650f93Test https://fundanet.igtp.cat/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=3398Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....521d7002c73abbb93e3d0174e5650f93 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219541 |
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