دورية أكاديمية
Using Metabolic Potential Within the Airway Microbiome as Predictors of Clinical State in Persons With Cystic Fibrosis
| العنوان: | Using Metabolic Potential Within the Airway Microbiome as Predictors of Clinical State in Persons With Cystic Fibrosis |
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| المؤلفون: | Shumyatsky, Gabriella, Burrell, Aszia, Chaney, Hollis, Sami, Iman, Koumbourlis, Anastassios C, Freishtat, Robert J, Crandall, Keith A, Zemanick, Edith T, Hahn, Andrea |
| المصدر: | Department of Bioscience Technologies Faculty Papers |
| بيانات النشر: | Jefferson Digital Commons |
| سنة النشر: | 2022 |
| المجموعة: | Jefferson Digital Commons (Thomas Jefferson University, Philadelphia) |
| مصطلحات موضوعية: | bacterial gene, cystic fibrosis, lung disease, metabolic pathway, microbiome, Biomedical Informatics, Health Information Technology, Medicine and Health Sciences |
| الوصف: | Introduction: Pulmonary exacerbations (PEx) in persons with cystic fibrosis (CF) are primarily related to acute or chronic inflammation associated with bacterial lung infections, which may be caused by several bacteria that activate similar bacterial genes and produce similar by-products. The goal of our study was to perform a stratified functional analysis of bacterial genes at three distinct time points in the treatment of a PEx in order to determine the role that specific airway microbiome community members may play within each clinical state (i.e., PEx, end of antibiotic treatment, and follow-up). Our secondary goal was to compare the change between clinical states with the metabolic activity of specific airway microbiome community members. Methods: This was a prospective observational study of persons with CF treated with intravenous antibiotics for PEx between 2016 and 2020 at Children's National Hospital. Demographic and clinical information as well as respiratory samples were collected at hospital admission for PEx, end of antibiotic treatment, and follow-up. Metagenomic sequencing was performed; MetaPhlAn3 and HUMANn3 were used to assign sequences to bacterial species and bacterial metabolic genes, respectively. Results: Twenty-two persons with CF, with a mean age of 14.5 (range 7-23) years, experienced 45 PEx during the study period. Two-hundred twenty-one bacterial species were identified in the respiratory samples from the study cohort. Ten bacterial species had differential gene abundance across changes in the clinical state including Staphylococcus aureus, Streptococcus salivarius, and Veillonella atypica (all padj < 0.01 and log2FoldChange > |2|). These corresponded to a differential abundance of bacterial genes, with S. aureus accounting for 81% of the genes more abundant in PEx and S. salivarius accounting for 83% of the genes more abundant in follow-up, all compared to the end of treatment. Lastly, 8,653 metabolic pathways were identified across samples, with again S. aureus and S. ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://jdc.jefferson.edu/bioscience_technologiesfp/5Test; https://jdc.jefferson.edu/cgi/viewcontent.cgi?article=1005&context=bioscience_technologiesfpTest |
| الإتاحة: | https://jdc.jefferson.edu/bioscience_technologiesfp/5Test https://jdc.jefferson.edu/cgi/viewcontent.cgi?article=1005&context=bioscience_technologiesfpTest |
| حقوق: | http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.A0CB5E59 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |