دورية أكاديمية
Combinatorial Regulation of Stilbene Synthase Genes by WRKY and MYB Transcription Factors in Grapevine ( Vitis vinifera L.)
| العنوان: | Combinatorial Regulation of Stilbene Synthase Genes by WRKY and MYB Transcription Factors in Grapevine ( Vitis vinifera L.) |
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| المؤلفون: | Vannozzi, Alessandro, Wong, Darren, Holl, Janine, Hmmam, Ibrahim, Matus, Jose Tomas, Bogs, Jochen, Ziegler, Tobias, Dry, I.B., Barcaccia, Gianni, Lucchin, Margherita |
| المصدر: | Plant and Cell Physiology |
| بيانات النشر: | Japanese Society of Plant Physiologists |
| المجموعة: | Australian National University: ANU Digital Collections |
| مصطلحات موضوعية: | Gene co-expression, Network, Resveratrol, Vitis vinifera |
| الوصف: | Stilbene synthase (STS) is the key enzyme leading to the biosynthesis of resveratrol. Recently we reported two R2R3-MYB transcription factor (TF) genes that regulate the stilbene biosynthetic pathway in grapevine: VviMYB14 and VviMYB15. These genes are strongly co-expressed with STS genes under a range of stress and developmental conditions, in agreement with the specific activation of STS promoters by these TFs. Genome-wide gene co-expression analysis using two separate transcriptome compendia based on microarray and RNA sequencing data revealed that WRKY TFs were the top TF family correlated with STS genes. On the basis of correlation frequency, four WRKY genes, namely VviWRKY03, VviWRKY24, VviWRKY43 and VviWRKY53, were further shortlisted and functionally validated. Expression analyses under both unstressed and stressed conditions, together with promoter–luciferase reporter assays, suggested different hierarchies for these TFs in the regulation of the stilbene biosynthetic pathway. In particular, VviWRKY24 seems to act as a singular effector in the activation of the VviSTS29 promoter, while VviWRKY03 acts through a combinatorial effect with VviMYB14, suggesting that these two regulators may interact at the protein level as previously reported in other species. ; This work was supported by the Cariparo Foundation [Cariparo Starting Grants] and the Department of Agronomy, Food, Natural Resources, Animals, and Environment (DAFNAE), University of Padova. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0032-0781 |
| العلاقة: | http://hdl.handle.net/1885/251147Test; https://openresearch-repository.anu.edu.au/bitstream/1885/251147/3/01_Vannozzi_Combinatorial_Regulation_of_2018.pdf.jpgTest |
| DOI: | 10.1093/pcp/pcy045 |
| الإتاحة: | https://doi.org/10.1093/pcp/pcy045Test http://hdl.handle.net/1885/251147Test https://openresearch-repository.anu.edu.au/bitstream/1885/251147/3/01_Vannozzi_Combinatorial_Regulation_of_2018.pdf.jpgTest |
| حقوق: | © The Author(s) 2018. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. |
| رقم الانضمام: | edsbas.6E798317 |
| قاعدة البيانات: | BASE |
| تدمد: | 00320781 |
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| DOI: | 10.1093/pcp/pcy045 |