Transplantation of Bone Marrow-Derived Mesenchymal Stem Cells into the Developing Mouse Eye
| العنوان: | Transplantation of Bone Marrow-Derived Mesenchymal Stem Cells into the Developing Mouse Eye |
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| المؤلفون: | Song-Hee Yu, Dong-Youn Hwang, Chang-Jin Jeon, Yu-Jin Jang, Eun-Shil Lee |
| المصدر: | Acta Histochemica et Cytochemica |
| بيانات النشر: | Japan Society of Histochemistry & Cytochemistry, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | retina, Pathology, medicine.medical_specialty, Retina, Histology, Physiology, Mesenchymal stem cell, Regular Article, integration, differentiation, Cell Biology, Biology, Biochemistry, In vitro, Pathology and Forensic Medicine, Green fluorescent protein, Transplantation, medicine.anatomical_structure, Microtubule-associated protein 2, bone marrow-derived mesenchymal stem cells, medicine, Bone marrow, transplantation, Stem cell transplantation for articular cartilage repair |
| الوصف: | Mesenchymal stem cells (MSCs) have been studied widely for their potential to differentiate into various lineage cells including neural cells in vitro and in vivo. To investigate the influence of the developing host environment on the integration and morphological and molecular differentiation of MSCs, human bone marrow-derived mesenchymal stem cells (BM-MSCs) were transplanted into the developing mouse retina. Enhanced green fluorescent protein (GFP)-expressing BM-MSCs were transplanted by intraocular injections into mice, ranging in ages from 1 day postnatal (PN) to 10 days PN. The survival dates ranged from 7 days post-transplantation (DPT) to 28DPT, at which time an immunohistochemical analysis was performed on the eyes. The transplanted BM-MSCs survived and showed morphological differentiation into neural cells and some processes within the host retina. Some transplanted cells expressed microtubule associated protein 2 (MAP2ab, marker for mature neural cells) or glial fibrillary acid protein (GFAP, marker for glial cells) at 5PN 7DPT. In addition, some transplanted cells integrated into the developing retina. The morphological and molecular differentiation and integration within the 5PN 7DPT eye was greater than those of other-aged host eye. The present findings suggest that the age of the host environment can strongly influence the differentiation and integration of BM-MSCs. |
| تدمد: | 1347-5800 0044-5991 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a34cdf48a147bac7855ff215284a0879Test https://doi.org/10.1267/ahc.11009Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a34cdf48a147bac7855ff215284a0879 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13475800 00445991 |
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