Evidence for Muscarinic 3 Receptor Mediated Ion Transport in HT29 Cells Studied by X-ray Microanalysis
| العنوان: | Evidence for Muscarinic 3 Receptor Mediated Ion Transport in HT29 Cells Studied by X-ray Microanalysis |
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| المؤلفون: | Wei Zhang, Godfried M. Roomans |
| المصدر: | Cell Structure and Function. 22:379-385 |
| بيانات النشر: | Japan Society for Cell Biology, 1997. |
| سنة النشر: | 1997 |
| مصطلحات موضوعية: | endocrine system, Carbachol, Physiology, medicine.drug_class, Muscarinic Agonists, Muscarinic acetylcholine receptor, Muscarinic acetylcholine receptor M5, medicine, Humans, Receptor, Molecular Biology, Ion Transport, Chemistry, Muscarinic acetylcholine receptor M3, Cell Biology, General Medicine, Muscarinic acetylcholine receptor M1, Receptor antagonist, Receptors, Muscarinic, Acetylcholine, Biochemistry, Biophysics, HT29 Cells, Electron Probe Microanalysis, Signal Transduction, medicine.drug |
| الوصف: | Changes in elemental content in response to muscarinic drugs in HT29 cells were investigated by X-ray microanalysis. Acetylcholine (ACh) and carbachol (Cch), both agonists binding to muscarinic receptors, induced a decrease of the intracellular Cl and K content. This agrees with the notion that these agonists induce electrolyte and water secretion. Atropine, a non-selective antagonist of muscarinic receptors, inhibited the decrease in K and Cl caused by ACh and Cch, and instead caused an increase of the Cl and K concentrations. A similar inhibition was found in the case of the selective muscarinic 3 receptor antagonist P-F-HHSiD. In contrast, the selective muscarinic 2 receptor antagonist AF-DX 116 did not inhibit Cch-activated secretion of K and Cl. A slight inhibition of ACh induced ion secretion was seen, but this inhibition was weak compared to that caused by P-F-HHSiD. Treatment with U-73122, an inhibitor of phospholipase C, blocked ACh or Cch induced ion secretion. These results suggest that ACh and Cch stimulated secretion of Cl and K is mediated by muscarinic 3 receptors via the inositol-1, 4, 5-trisphosphate (IP3) dependent pathway. |
| تدمد: | 1347-3700 0386-7196 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea159123f0f93d6ec3ed8803258e3c59Test https://doi.org/10.1247/csf.22.379Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ea159123f0f93d6ec3ed8803258e3c59 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13473700 03867196 |
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