Production of mouse monoclonal antibodies against human HLA-A3 for use in microlymphocytotoxicity technique
| العنوان: | Production of mouse monoclonal antibodies against human HLA-A3 for use in microlymphocytotoxicity technique |
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| المؤلفون: | Zohreh Sharifi, Shahla Sareh, Freshteh Najafi, Azadeh Sadri, Fatemeh Yari |
| المصدر: | Human Antibodies. 18:119-122 |
| بيانات النشر: | IOS Press, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Cytotoxicity, Immunologic, medicine.drug_class, Immunology, Enzyme-Linked Immunosorbent Assay, Human leukocyte antigen, HLA-A3 Antigen, Monoclonal antibody, HAT medium, Mice, HLA-A3, Antigen, Cell Adhesion, medicine, Animals, Humans, Immunology and Allergy, Lymphocytes, Alleles, Mice, Inbred BALB C, Hybridomas, biology, Antibodies, Monoclonal, General Medicine, Virology, Molecular biology, Culture Media, biology.protein, Hybridoma technology, Female, Antibody, Clone (B-cell biology), Spleen |
| الوصف: | Monoclonal antibody production using the hybridoma technology is one of the attractive areas of research. The aim of this study was to investigate the production of monoclonal antibodies to human HLA-A3 antigen for use in microlymphocytotoxicity technique. HLA-A3 antigen was purified from human leukocytes and injected to several mice. The spleen-derived cells of immunized animal were fused with P3-X63-Ag8 myeloma cells. Obtained hybridoma cells were grown in HAT medium and the supernatant of the cells were screened for antibodies using ELISA and microlymphocytotoxicity technique. Produced monoclonal antibody from the hybridoma clone, B1F10 showed the specificity for human HLA-A3. Furthermore, in this study, we encountered with clones of immortal hybrid cells with completely different features, they were adherent and non-producers for antibodies. This observation could imply the possible role of the antigen (HLA) and its nature in the expansion of cell types other than B cells in the spleen of mouse during immunization. |
| تدمد: | 1875-869X 1093-2607 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b001ad85be984e840bdcb2a451e2683Test https://doi.org/10.3233/hab-2009-0201Test |
| رقم الانضمام: | edsair.doi.dedup.....6b001ad85be984e840bdcb2a451e2683 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1875869X 10932607 |
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