Enhanced Neurite Outgrowth and Branching Precede Increased Amyloid-β-Induced Neuronal Apoptosis in a Novel Alzheimer's Disease Model
| العنوان: | Enhanced Neurite Outgrowth and Branching Precede Increased Amyloid-β-Induced Neuronal Apoptosis in a Novel Alzheimer's Disease Model |
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| المؤلفون: | Daniel L. Segal, Yaara Saad, Amir Ayali |
| المصدر: | Journal of Alzheimer's Disease. 43:993-1006 |
| بيانات النشر: | IOS Press, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Nervous system, Neurite, Apoptosis, Animals, Genetically Modified, Alzheimer Disease, Neurites, medicine, Extracellular, Animals, Viability assay, Cells, Cultured, Neurons, Amyloid beta-Peptides, biology, General Neuroscience, Neurodegeneration, General Medicine, medicine.disease, biology.organism_classification, Peptide Fragments, Cell biology, Disease Models, Animal, Psychiatry and Mental health, Clinical Psychology, Drosophila melanogaster, medicine.anatomical_structure, Cell culture, Geriatrics and Gerontology, Intracellular |
| الوصف: | Though it is widely accepted that amyloid-β (Aβ) is a key factor in Alzheimer's disease (AD) pathology, its underlying mechanism remains unclear. In order to study the association between Aβ and neural circuitry dysfunction, we developed a primary culture preparation derived from the nervous system of transgenic Drosophila melanogaster larvae expressing human Aβ1-42 (Aβ42). Cultured neurons undergo a consistent developmental process, culminating in an elaborate neuronal network with distinct functional and morphological characteristics. Throughout this development, a time-dependent increase in intracellular expression levels of Aβ42 was detected, followed by extracellular staining at a later time point. When compared to controls, Aβ42 cultures exhibited enhanced levels of apoptosis, resulting in reduced cell viability. Moreover, as primary culture preparations enable high resolution monitoring of neuronal phenotypes, we were able to detect subtle morphological changes in neurons expressing Aβ42, namely an enhancement in neurite outgrowth and arborization, which preceded the effect of neurodegeneration. Our results establish D. melanogaster primary neuronal cultures as a rapid, accessible and cost-effective platform for AD molecular studies and drug screening, and suggest a possible role for Aβ42 in the organization of neuronal processes. |
| تدمد: | 1875-8908 1387-2877 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4bd3245483297694b13aeb940950800Test https://doi.org/10.3233/jad-140009Test |
| رقم الانضمام: | edsair.doi.dedup.....f4bd3245483297694b13aeb940950800 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18758908 13872877 |
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