Dynamic regulation of mechanosensitive channels: capacitance used to monitor patch tension in real time
| العنوان: | Dynamic regulation of mechanosensitive channels: capacitance used to monitor patch tension in real time |
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| المؤلفون: | Frederick Sachs, Thomas M. Suchyna, Steven R Besch |
| المصدر: | Physical Biology. 1:1-18 |
| بيانات النشر: | IOP Publishing, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Patch-Clamp Techniques, Biophysics, Analytical chemistry, In Vitro Techniques, Electric Capacitance, Mechanotransduction, Cellular, Models, Biological, Biophysical Phenomena, Ion Channels, Rats, Sprague-Dawley, Structural Biology, Animals, Patch clamp, Mechanotransduction, Molecular Biology, Cytoskeleton, Ion channel, Membrane potential, Voltage-gated ion channel, Chemistry, Depolarization, Cell Biology, Rats, Stretch-activated ion channel, Astrocytes, Mechanosensitive channels, Ion Channel Gating |
| الوصف: | All cells, from bacteria to human, are mechanically sensitive. The most rapid of these membrane protein transducers are mechanosensitive ion channels, ionic pores in the membrane that open and close in response to membrane tension. In specific sensory organs, these channels serve the senses of touch and hearing, and inform the central nervous system about the filling of hollow organs such as the bladder. Non-specialized cells use these channels to report on changes in cell volume and local strain. To preserve dynamic sensitivity, sensory receptors adapt to steady-state stimuli. Here we show that in rat astrocytes, the most abundant cells in the brain, this apparent adaptation to the stimulus is actually an inactivation. We have been able to track the time course of local strain by measuring attofarad changes in membrane capacitance and show that it is not correlated with loss of channel activity. The reduction in current with time is caused by an increased occupancy of low conductance states, and a reduction in the probability of opening, not a relaxation of local stress. The occupancy of these substates depends on the integrity of the cell's cytoplasm. However, while disruption of the cytoskeleton leads to a loss of inactivation, it leaves activation unaffected. The activation process is voltage-insensitive, closely correlated with changes in capacitance, and seems to arise solely from stress in the bilayer. The inactivation rate decreases with depolarization, and kinetic analysis suggests that the process involves multiple cytoplasmic ligands. Surprisingly, multivalent ions such as Gd(+3) and Ca(+2) that bind to the lipids and affect channel gating, do not affect the strain-induced increase in membrane capacitance; contrary to expectations, membrane elasticity is unchanged. |
| تدمد: | 1478-3975 1478-3967 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98a05e52cc6e476201cd2ec463b8e81aTest https://doi.org/10.1088/1478-3967/1/1/001Test |
| رقم الانضمام: | edsair.doi.dedup.....98a05e52cc6e476201cd2ec463b8e81a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14783975 14783967 |
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