Facile synthesis of bioactive Allitol from D-psicose by coexpression of ribitol dehydrogenase and formate dehydrogenase in Escherichia Coli
| العنوان: | Facile synthesis of bioactive Allitol from D-psicose by coexpression of ribitol dehydrogenase and formate dehydrogenase in Escherichia Coli |
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| المؤلفون: | Mohammed A.A. Eassa, Hinawi A.M. Hassanin, Bo Jiang |
| المصدر: | Journal of Food Bioactives. 4 |
| بيانات النشر: | International Society for Nutraceuticals & Functional Foods (ISNFF), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Psicose, chemistry.chemical_compound, Biochemistry, chemistry, Ribitol dehydrogenase, Yield (chemistry), medicine, NADH regeneration, Substrate (chemistry), Formate dehydrogenase, medicine.disease_cause, Escherichia coli, Substrate concentration |
| الوصف: | Coexpression of formate dehydrogenase (FDH) and ribitol dehydrogenase (RDH) in Escherichia coli was used for the synthesis of Allitol from D-psicose. FDH was coexpressed with RDH for continuous NADH regeneration. The results revealed that the optimum conditions for allitol production occurred at pH 7.0 and 30 °C. Allitol reached the maximum yield of 19.2 mg at 2.0% substrate concentration after 48 hours. Using D-psicose as a substrate, allitol was successfully produced using an engineered E. coli coexpressed with RDH and FDH. |
| تدمد: | 2637-8779 2637-8752 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::ac1ce00316c670611009a7b3f5c6d563Test https://doi.org/10.31665/jfb.2018.4167Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........ac1ce00316c670611009a7b3f5c6d563 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 26378779 26378752 |
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