دورية أكاديمية
Hydrostatic Compress Force Enhances the Viability and Decreases the Apoptosis of Condylar Chondrocytes through Integrin-FAK-ERK/PI3K Pathway
| العنوان: | Hydrostatic Compress Force Enhances the Viability and Decreases the Apoptosis of Condylar Chondrocytes through Integrin-FAK-ERK/PI3K Pathway |
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| المؤلفون: | Ma, Dandan, Kou, Xiaoxing, Jin, Jing, Xu, Taotao, Wu, Mengjie, Deng, Liquan, Fu, Lusi, Liu, Yi, Wu, Gang, Lu, Haiping |
| المساهمون: | Lu, HP (reprint author), Zhejiang Chinese Med Univ, Stomatol, Hangzhou 310053, Zhejiang, Peoples R China., Wu, G (reprint author), Vrije Univ Amsterdam, Acad Ctr Dent Amsterdam ACTA, Dept Oral Implantol & Prosthet Dent, Gustav Mahlerlaan 3004, NL-1081 LA Amsterdam, Nord Holland, Netherlands., Wu, G (reprint author), Univ Amsterdam, MOVE Res Inst, Gustav Mahlerlaan 3004, NL-1081 LA Amsterdam, Nord Holland, Netherlands., Zhejiang Chinese Med Univ, Stomatol, Hangzhou 310053, Zhejiang, Peoples R China., Vrije Univ Amsterdam, Acad Ctr Dent Amsterdam ACTA, Dept Oral Implantol & Prosthet Dent, Gustav Mahlerlaan 3004, NL-1081 LA Amsterdam, Nord Holland, Netherlands., Univ Amsterdam, MOVE Res Inst, Gustav Mahlerlaan 3004, NL-1081 LA Amsterdam, Nord Holland, Netherlands., Peking Univ, Sch & Hosp Stomatol, Dept Orthodont, Beijing 100081, Peoples R China., Zhejiang Chinese Med Univ, Affiliated Hosp 1, Dept Orthopaed, Hangzhou 310006, Zhejiang, Peoples R China., Zhejiang Univ, Stomatol Hosp, Dept Orthodont, Hangzhou 310053, Zhejiang, Peoples R China. |
| المصدر: | SCI |
| بيانات النشر: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
| سنة النشر: | 2016 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | integrin, FAK, ERK, PI3K, mandibular condyle, chondrocyte, apoptosis, FOCAL ADHESION KINASE, PERIODONTAL-LIGAMENT FIBROBLASTS, EXTRACELLULAR-MATRIX, RAT CHONDROCYTES, GLYCOSAMINOGLYCAN SYNTHESIS, HYPERTROPHIC CHONDROCYTES, ARTICULAR CHONDROCYTES, SIGNAL-TRANSDUCTION, MITOGENIC SIGNAL |
| الوصف: | Reduced mechanical stimuli in many pathological cases, such as hemimastication and limited masticatory movements, can significantly affect the metabolic activity of mandibular condylar chondrocytes and the growth of mandibles. However, the molecular mechanisms for these phenomena remain unclear. In this study, we hypothesized that integrin-focal adhesion kinase (FAK)-ERK (extracellular signal-regulated kinase)/PI3K (phosphatidylinositol-3-kinase) signaling pathway mediated the cellular response of condylar chondrocytes to mechanical loading. Primary condylar chondrocytes were exposed to hydrostatic compressive forces (HCFs) of different magnitudes (0, 50, 100, 150, 200, and 250 kPa) for 2 h. We measured the viability, morphology, and apoptosis of the chondrocytes with different treatments as well as the gene, protein expression, and phosphorylation of mechanosensitivity-related molecules, such as integrin alpha 2, integrin alpha 5, integrin beta 1, FAK, ERK, and PI3K. HCFs could significantly increase the viability and surface area of condylar chondrocytes and decrease their apoptosis in a dose-dependent manner. HCF of 250 kPa resulted in a 1.51 +/- 0.02-fold increase of cell viability and reduced the ratio of apoptotic cells from 18.10% +/- 0.56% to 7.30% +/- 1.43%. HCFs could significantly enhance the mRNA and protein expression of integrin alpha 2, integrin alpha 5, and integrin beta 1 in a dose-dependent manner, but not ERK1, ERK2, or PI3K. Instead, HCF could significantly increase phosphorylation levels of FAK, ERK1/2, and PI3K in a dose-dependent manner. Cilengitide, the potent integrin inhibitor, could dose-dependently block such effects of HCFs. HCFs enhances the viability and decreases the apoptosis of condylar chondrocytes through the integrin-FAK-ERK/PI3K pathway. ; National Natural Science Foundation of China [81400475, 81470724, 81500813]; Zhejiang Provincial Natural Science Foundation of China [LY14H140006, LY14H140002, LY17H140010] ; SCI(E) ; ARTICLE ; whiterose1989@126.com; kouxiaoxing@gmail.com; ... |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-00-388809-6 0-00-388809-6 |
| تدمد: | 1422-0067 |
| العلاقة: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.2016,17(11).; 1516817; http://hdl.handle.net/20.500.11897/458562Test; WOS:000388809600080 |
| DOI: | 10.3390/ijms17111847 |
| الإتاحة: | https://doi.org/20.500.11897/458562Test https://doi.org/10.3390/ijms17111847Test https://hdl.handle.net/20.500.11897/458562Test |
| رقم الانضمام: | edsbas.5FBAE335 |
| قاعدة البيانات: | BASE |
Full Text Finder | ردمك: | 9780003888096 0003888096 |
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| تدمد: | 14220067 |
| DOI: | 10.3390/ijms17111847 |