دورية أكاديمية
Expression of hiwi gene in human gastric cancer was associated with proliferation of cancer cells
| العنوان: | Expression of hiwi gene in human gastric cancer was associated with proliferation of cancer cells |
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| المؤلفون: | Liu, XY, Sun, Y, Guo, JP, Ma, H, Li, JY, Dong, B, Jin, GL, Zhang, JZ, Wu, J, Meng, L, Shou, CC |
| المساهمون: | Shou, CC (reprint author), Peking Univ, Sch Oncol, Beijing Inc Canc Res, Dept Biochem & Mol Biol, 1 Hong Luo Chang St, Beijing 100034, Peoples R China., Peking Univ, Sch Oncol, Beijing Inc Canc Res, Dept Biochem & Mol Biol, Beijing 100034, Peoples R China., Peking Univ, Dept Pathol, Sch Oncol, Beijing Canc Hosp, Beijing, Peoples R China., Peking Univ, Sch Oncol, Beijing Inc Canc Res, Dept Biochem & Mol Biol, 1 Hong Luo Chang St, Beijing 100034, Peoples R China. |
| المصدر: | PubMed ; SCI |
| بيانات النشر: | international journal of cancer |
| سنة النشر: | 2006 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | hiwi, expression, gastric cancer, proliferation, STEM-CELL, PIWI, FAMILY |
| الوصف: | Stem cell genetics research may be critical to our understanding of carcinogenesis, as both stem cells and cancer cells possess the ability to self-renew. Recent discoveries have indicated that the piwi family of genes plays an essential role in stem cell self-renewal in diverse organisms. The hiwi gene, the human homolog of the piwi family, participates in germ cell proliferation and its overexpression may cause the development of germ cell malignancy, but its expression and function in epithelial solid cancers have not been explored. In the present study, we investigated whether there was an association between hiwi expression and human gastric cancer and its potential mechanism. RT-PCR findings demonstrated that hiwi was expressed in different gastric cancer cell lines. To identify the HIWI protein in gastric cancer, we developed a specific monoclonal antibody against HIWI and immunohistochemistry was performed on various gastric tissues. We found that the expression ratio of hiwi in normal gastric tissues, atrophic gastritis, intestinal metaplasia and gastric cancers was 10% (5/50), 36% (18/50), 36% (18/50) and 76% (38/50), respectively, which was consistent with precancerous development. Notably, the expression pattern of hiwi in gastric cancer tissues was similar to that of Ki67, which was used as a marker of proliferation. Moreover, the suppression of hiwi by antisense or RNAi inhibited the growth of gastric cancer cells and induced cell cycle arrest in G(2)/M phase. These results suggest that hiwi may be involved in the development of gastric cancer and is a potential target for cancer therapy. (c) 2005 Wiley-Liss, Inc. ; Oncology ; SCI(E) ; PubMed ; 73 ; ARTICLE ; 8 ; 1922-1929 ; 118 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 0020-7136 |
| العلاقة: | INTERNATIONAL JOURNAL OF CANCER.2006,118,(8),1922-1929.; 675210; http://hdl.handle.net/20.500.11897/199023Test; WOS:000236397200010 |
| DOI: | 10.1002/ijc.21575 |
| الإتاحة: | https://doi.org/20.500.11897/199023Test https://doi.org/10.1002/ijc.21575Test https://hdl.handle.net/20.500.11897/199023Test |
| رقم الانضمام: | edsbas.679BCF9B |
| قاعدة البيانات: | BASE |
| تدمد: | 00207136 |
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| DOI: | 10.1002/ijc.21575 |