microRNA-99a Reduces Lipopolysaccharide-Induced Oxidative Injury by Activating Notch Pathway in H9c2 Cells
| العنوان: | microRNA-99a Reduces Lipopolysaccharide-Induced Oxidative Injury by Activating Notch Pathway in H9c2 Cells |
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| المؤلفون: | Jing Ran, Zhengming Zhou, Kuang Feng, Chuanchang Li, Huang Lei |
| المصدر: | International Heart Journal. 58:422-427 |
| بيانات النشر: | International Heart Journal (Japanese Heart Journal), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Lipopolysaccharides, 0301 basic medicine, Programmed cell death, Lipopolysaccharide, Notch signaling pathway, Apoptosis, Myocardial Reperfusion Injury, 030204 cardiovascular system & hematology, Real-Time Polymerase Chain Reaction, Flow cytometry, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Lactate dehydrogenase, medicine, Animals, Myocytes, Cardiac, Cells, Cultured, Caspase, chemistry.chemical_classification, Reactive oxygen species, medicine.diagnostic_test, biology, business.industry, General Medicine, Flow Cytometry, Rats, Cell biology, Disease Models, Animal, MicroRNAs, Oxidative Stress, 030104 developmental biology, Gene Expression Regulation, chemistry, biology.protein, RNA, Reactive Oxygen Species, Cardiology and Cardiovascular Medicine, business, Signal Transduction |
| الوصف: | microRNA-99a (miR-99a) is recently recognized as a key regulator in various cancers and cardiovascular diseases. In the present study, we sought to investigate the effects of miR-99a in rat cardiomyocyte H9c2 cells against oxidative injury induced by lipopolysaccharide (LPS).MTT assay, reactive oxygen species (ROS) assay, flow cytometry and lactate dehydrogenase (LDH) assay were respectively used to explore viability, ROS levels, apoptosis, and cell death in H9c2 cells. Quantitative PCR (qRT-PCR) was performed to confirm the expression of miR-99a. Western blot was performed to determine the expression of Notch pathway factors.LPS could significantly suppress viability and increase cell death, apoptosis, and ROS level (P < 0.05). However, miR-99a could significantly increase the viability and decrease apoptosis and ROS level of H9c2 cells (P < 0.05). Overexpression of miR-99a could activate a Notch pathway and regulate the expression of B-cell CLL/lymphoma 2 (BCL2) and cleaved caspase 3.Our study found that overexpression of miR-99a could attenuate LPS-induced oxidative injury in H9c2 cells, possibly via a Notch pathway. These findings suggest that miR-99a may be a key factor in cardiomyocyte oxidative injury and could be a new therapeutic strategy for cardiovascular diseases. |
| تدمد: | 1349-3299 1349-2365 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d076b45366a27ca71b868a86d56c800fTest https://doi.org/10.1536/ihj.16-261Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d076b45366a27ca71b868a86d56c800f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13493299 13492365 |
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