كتاب
Clustering of olive pollens into model cultivars on the basis of their allergenic content
| العنوان: | Clustering of olive pollens into model cultivars on the basis of their allergenic content |
|---|---|
| المؤلفون: | Morales, Sonia, Castro, Antonio Jesús, Salmerón, Carmen, Marco, Francisco M., Rodríguez García, María Isabel, Alché, Juan de Dios |
| المساهمون: | Universidad de Alicante. Departamento de Biotecnología, Grupo de Inmunología |
| بيانات النشر: | InTech |
| سنة النشر: | 2012 |
| المجموعة: | RUA - Repositorio Institucional de la Universidad de Alicante |
| مصطلحات موضوعية: | Olea europaea L, Pollen, Allergens, Alllergic, Inmunología |
| الوصف: | Olive germplasm is exceptionally wide, with more than 250 cultivars only in Spain (Barranco and Rallo, 2005). Olive cultivars display strong differences in the allergenic content of their pollen, which are particularly relevant in the case of several allergens like Ole e 1 (the olive pollen major allergen). These changes affect the total biological potency of the extracts used for clinics. Other clinical aspects like patient's reactivity to the individualized allergens and potential safety of the extracts are also influenced (Alché et al., 2007; this volume). Cultivar origin is also a major cause of the reported polymorphism affecting most olive pollen characterized allergens. This distinctiveness is maintained over the years and is a direct consequence of the genetic background of each cultivar. Manufacturers of clinical pollen extracts used for diagnosis and immunotherapy are starting to consider cultivar origin of the pollen in their standardization procedures, yet commercial olive pollen used for clinical and biological assays does not disclose in most cases its cultivar origin. As handling and characterization of a large number of cultivar extracts is impracticable under industrial and clinical standards, the present work intends to define a limited number of cultivar models, characterized by distinctive pollen allergen profiles. With this purpose, the content of four relevant allergens (Ole e 1, Ole e 2, Ole e 5 and Ole e 9) was analyzed in commercial extracts prepared from pollen of eleven olive cultivars by SDS-PAGE and immunoblotting, using specific antibodies. Allergen semi-quantization was performed by densitometry using the Quantity One program (Bio-Rad Laboratories). Densitometry data were made comparative among cultivars, by referring the cultivar with the higher concentration of a given allergen as to 100%. Thresholds percentages were defined in order to characterize individual levels of an allergen for each cultivar as low, average and high, and the following categories were established: - ... |
| نوع الوثيقة: | book part |
| اللغة: | English |
| ردمك: | 978-953-51-0860-3 953-51-0860-3 |
| العلاقة: | http://dx.doi.org/10.5772/49964Test; Sonia Morales, Antonio Jesús Castro, Carmen Salmerón, Francisco Manuel Marco, María Isabel Rodríguez-García and Juan de Dios Alche (2012). Clustering of Olive Pollens into Model Cultivars on the Basis of Their Allergenic Content, Current Insights in Pollen Allergens, Dr. Jose C. Jimenez-Lopez (Ed.), ISBN: 978-953-51-0860-3, InTech, DOI:10.5772/49964. Available from: http://www.intechopen.com/books/current-insights-in-pollen-allergens/clustering-of-olive-pollens-into-model-cultivars-on-the-basis-of-their-allergenic-contentTest; http://hdl.handle.net/10045/34935Test; A7277246 |
| DOI: | 10.5772/49964 |
| الإتاحة: | https://doi.org/10.5772/49964Test http://hdl.handle.net/10045/34935Test |
| حقوق: | © 2012 Morales et al., licensee InTech. This is an open access chapter distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0Test), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.822825B8 |
| قاعدة البيانات: | BASE |
| ردمك: | 9789535108603 9535108603 |
|---|---|
| DOI: | 10.5772/49964 |