دورية أكاديمية
Regulation of gene expression in plasmid vectors: doxocyclin-de- pendent and hypoxia-regulated systems ; Regulacja ekspresji genów w wektorach plazmidowych: system zależny od doksycykliny i regulowany przez niedotlenienie
| العنوان: | Regulation of gene expression in plasmid vectors: doxocyclin-de- pendent and hypoxia-regulated systems ; Regulacja ekspresji genów w wektorach plazmidowych: system zależny od doksycykliny i regulowany przez niedotlenienie |
|---|---|
| المؤلفون: | Gołda, Sławomir, Kucharzewska, Paulina, Cisowski, Jarosław, Florczyk, Urszula, Zagórska, Anna, Jaźwa, Agnieszka, Łoboda, Agnieszka, Józkowicz, Alicja, Dulak, Józef |
| المصدر: | Library of Institute of Bioorganic Chemistry PAS ; Biblioteka Instytutu Chemii Bioorganicznej PAN |
| بيانات النشر: | Committee on Biotechnology PAS Institute of Bioorganic Chemistry PAS Komitet Biotechnologii PAN Instytut Chemii Bioorganicznej PAN |
| سنة النشر: | 2007 |
| المجموعة: | Digital Repository of Scientific Institutes (RCIN) |
| مصطلحات موضوعية: | biotechnology, biotechnologia |
| الوصف: | Regulation of gene expression in gene therapy is crucial for obtaining the therapeutic effects, thanks to limitation of transgene activity to the selected cells in a given time. In this paper we have focused on plasmid expression systems regulated by doxycycline or hypoxia. We have described in details the structure, regulatory elements and biological applications of 1) the modified, commercially available Tet-On system, expressing doxycycline-controlled P-galactosidase and 2) hypoxia-activated FGF-4A^GF expression plasmid containing the hypoxia responsive sequence. The presented expression systems can also be used in viral vectors, enabling not only regulated, but also high and long-term expression of transgenes. ; Regulation of gene expression in gene therapy is crucial for obtaining the therapeutic effects, thanks to limitation of transgene activity to the selected cells in a given time. In this paper we have focused on plasmid expression systems regulated by doxycycline or hypoxia. We have described in details the structure, regulatory elements and biological applications of 1) the modified, commercially available Tet-On system, expressing doxycycline-controlled P-galactosidase and 2) hypoxia-activated FGF-4A^GF expression plasmid containing the hypoxia responsive sequence. The presented expression systems can also be used in viral vectors, enabling not only regulated, but also high and long-term expression of transgenes. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | Polish |
| العلاقة: | Biotechnologia, vol.78, 3 (2007)-.; oai:rcin.org.pl:publication:100057; https://rcin.org.pl/dlibra/publication/edition/76141/contentTest; oai:rcin.org.pl:76141 |
| الإتاحة: | https://rcin.org.pl/dlibra/publication/edition/76141/contentTest |
| حقوق: | Creative Commons Attribution BY-SA 4.0 license ; Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0 |
| رقم الانضمام: | edsbas.D79A38 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |