رسالة جامعية
Tissue responses to bone-implant biomaterials
| العنوان: | Tissue responses to bone-implant biomaterials |
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| المؤلفون: | Liao, Haihong |
| بيانات النشر: | Institutionen för odontologi / Department of Odontology |
| سنة النشر: | 1999 |
| المجموعة: | Karolinska Institutet: Publications |
| مصطلحات موضوعية: | Gene expression, interface, mineralization, nacre, osteoblast, osteoclast, titanium |
| الوصف: | The purpose of the studies was to understand the mechanisms which underlie the interaction between tissue and bone-implant biomaterials. Tissue and naturally occurring aragonite nacre interaction was systematically studied for the first time by combined histological examination, cellular and molecular biological approaches. The performance of titanium, its surface oxide layer and titanium ion in vitro were also within the scope of the present thesis. A matched resorption of nacre and ingrowth of new bone was found by histological examination in vivo. Bone was formed in an osteoconductive manner and tightly bonded with nacre, as for with the bioactive hydroxyapatite. An intermediate phosphorous-rich layer was detected at the bone-nacre interface. The different reactions of bone to nacre and to titania-hydroxyapaptite indicated that the surface of implant material exerted effect on both osteogenic and soft-tissue cells as examined by in situ hybridisation. However, the nacre implant surface did not exert a significant stimulatory effect on osteogenesis, and the bone-nacre bonding was achieved by the favourable surface provided by nacre for bone cell proliferation and bone matrix to attach. Degradation of nacre was mediated by a combination of cellular and physico-chemical dissolution processes. Macrophages and multinucleated giant cells were shown to be responsible for the cellular degradation process. These cells exhibited different molecular profiles when compared in soft and osseous tissue, i.e., the cells expressed high level of osteopontin mRNA in soft tissue and less at the osseous site. The multinucleated giant cells did not represent typical osteoclasts, since their size, degradation efficiency and expression of tartrate resistance acid phosphate mRNA and tartrate resistance acid phosphate enzyme activity were different from that of osteoclasts. We also examined the structural and compositional influence of cultured osteoblasts on titanium surface, together with the effect of hydrogen peroxide treatment. ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| وصف الملف: | paper |
| اللغة: | unknown |
| ردمك: | 978-91-628-3564-4 91-628-3564-5 |
| العلاقة: | 91-628-3564-5; 19990604liao; http://hdl.handle.net/10616/44170Test |
| الإتاحة: | http://hdl.handle.net/10616/44170Test |
| رقم الانضمام: | edsbas.3655EBDD |
| قاعدة البيانات: | BASE |
| ردمك: | 9789162835644 9162835645 |
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