دورية أكاديمية
The complement regulator CD46 is bactericidal to Helicobacter pylori and blocks urease activity
| العنوان: | The complement regulator CD46 is bactericidal to Helicobacter pylori and blocks urease activity |
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| المؤلفون: | Basmarke-Wehelie, Rahma, Sjölinder, Hong, Jurkowski, Wiktor, Elofsson, Arne, Arnqvist, Anna, Engstrand, Lars, Hagner, Matthias, Wallin, Elin, Guan, Na, Kuranasekera, Hasanthi, Aro, Helena, Jonsson, Ann-Beth |
| بيانات النشر: | Institutionen för genetik, mikrobiologi och toxikologi Institutionen för biokemi och biofysik Umea Univ, Dept Med Biochem & Biophys, Sweden Karolinska Inst, Swedish Inst Infect Dis Control, Sweden |
| سنة النشر: | 2011 |
| المجموعة: | Stockholm University: Publications (DiVA) |
| مصطلحات موضوعية: | AhpC, Stomach Cancer, Bacterial Infection, Microbiology, Mikrobiologi, Gastroenterology and Hepatology, Gastroenterologi |
| الوصف: | BACKGROUND & AIMS: CD46 is a C3b/C4b binding complement regulator and a receptor for several human pathogens. We examined the interaction between CD46 and Helicobacter pylori (a bacterium that colonizes the human gastric mucosa and causes gastritis), peptic ulcers, and cancer. METHODS: Using gastric epithelial cells, we analyzed a set of H pylori strains and mutants for their ability to interact with CD46 and/or influence CD46 expression. Bacterial interaction with full-length CD46 and small CD46 peptides was evaluated by flow cytometry, fluorescence microscopy, enzyme-linked immunosorbent assay, and bacterial survival analyses. RESULTS: H pylori infection caused shedding of CD46 into the extracellular environment. A soluble form of CD46 bound to H pylori and inhibited growth, in a dose- and time-dependent manner, by interacting with urease and alkyl hydroperoxide reductase, which are essential bacterial pathogenicity-associated factors. Binding of CD46 or CD46-derived synthetic peptides blocked the urease activity and ability of bacteria to survive in acidic environments. Oral administration of one CD46 peptide eradicated H pylori from infected mice. CONCLUSIONS: CD46 is an antimicrobial agent that can eradicate H pylori. CD46 peptides might be developed to treat H pylori infection. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | Gastroenterology, 0016-5085, 2011, 141:3, s. 918-928; orcid:0000-0002-7115-9751; http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-63669Test; PMID 21699774; ISI:000294281200032 |
| DOI: | 10.1053/j.gastro.2011.05.009 |
| الإتاحة: | https://doi.org/10.1053/j.gastro.2011.05.009Test http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-63669Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.BDD2DBC7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1053/j.gastro.2011.05.009 |
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