التفاصيل البيبلوغرافية
| العنوان: |
Association of Nonpuerperal Mastitis with Cytokines Related to Helper T Cells TH1/TH2 and TH17/Treg. |
| المؤلفون: |
Jin Zhao1, Haoyang Ji1, Xiuhong Wang2, Yueqi Wang3, Zhongyuan Xia3 zryhyyxzy@163.com |
| المصدر: |
Alternative Therapies in Health & Medicine. Nov/Dec2023, Vol. 29 Issue 8, p150-155. 6p. |
| مصطلحات موضوعية: |
*MASTITIS, *CYTOKINES, *T helper cells, *BREAST diseases, *INTERLEUKINS, *IMMUNOFLUORESCENCE |
| مستخلص: |
Objective • To investigate the association of nonpuerperal mastitis with cytokines related to the helper T cells TH1/ TH2 and TH17/Treg and associated immune balance. Methods • From 2016 to 2021, we included 40 patients with non-puerperal mastitis who underwent surgery at ChinaJapan Friendship Hospital and compared them with 40 control patients with benign non-infectious breast disease. Hematoxylin-eosin staining detects inflammatory infiltrates of breast tissue. The expression of interferon γ and interleukin 4 in breast tissue was detected by immunofluorescence imaging, and the relative protein expression of TH1/TH2 and TH17/Treg cell-associated cytokines in CD4+ T cells was detected by western blotting. CD4+ T cells were isolated by fluorescence-activated cell sorting for detection of the relative protein expression of interferon γ and interleukin 4 in CD4+ T cells. Results • Hematoxylin-eosin staining showed that the nonpuerperal mastitis group had significantly greater inflammatory infiltration than the control group. Immunofluorescence images showed the relative fluorescence intensity of interferon γ was significantly higher in the nonpuerperal mastitis group than in the control group (P<.001), but the relative fluorescence intensity of interleukin 4 did not significantly differ between the 2 groups (P=.0686). Western blotting revealed that the relative protein expression of interferon γ, interleukin 2, and interleukin 17 was significantly higher in the nonpuerperal mastitis group than in the control group (P <.001), but the relative protein expression of interleukin 4 (P=.0512), interleukin 10 (P=.3088), and transforming growth factor β (P=.0653) did not significantly differ between the 2 groups. Flow cytometry of isolated CD4+ T cells showed the relative protein expression of interferon γ was significantly higher in the nonpuerperal mastitis group than in the control group (P <.001), but the relative protein expression of interleukin 4 did not significantly differ between the 2 groups (P=.0680). Conclusion • The expression of the TH1 cytokines interferon γ and interleukin 2 and the TH17 cytokine interleukin 17 was significantly higher in patients with nonpuerperal mastitis, while the TH2 cytokine interleukin 4 and the Treg cytokines interleukin 10 and transforming growth factor β were expressed at lower levels. This study provides new research ideas for the treatment of mastitis. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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