The Silk Protein Sericin Promotes Viability of ARPE-19 and Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cellsin vitro
| العنوان: | The Silk Protein Sericin Promotes Viability of ARPE-19 and Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cellsin vitro |
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| المؤلفون: | Jon Roger Eidet, Evan Michael Vallenari, Tor Paaske Utheim, Hans Christian Dalsbotten Aass, Ayyad Ahmad Zartasht Khan, Morten Carsten Moe, Dipak Sapkota |
| المصدر: | Current Eye Research. 46:504-514 |
| بيانات النشر: | Informa UK Limited, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | cis-trans-Isomerases, Cell Survival, Induced Pluripotent Stem Cells, Retinal Pigment Epithelium, Sericin, Cell Line, 03 medical and health sciences, Cellular and Molecular Neuroscience, Pigment, chemistry.chemical_compound, 0302 clinical medicine, medicine, Humans, Lactic Acid, Sericins, Induced pluripotent stem cell, Cells, Cultured, Retinal pigment epithelium, Monophenol Monooxygenase, Chemistry, food and beverages, Retinal, Hydrogen-Ion Concentration, Flow Cytometry, eye diseases, Sensory Systems, In vitro, Cell biology, Ophthalmology, Glucose, medicine.anatomical_structure, SILK, visual_art, Zonula Occludens-1 Protein, 030221 ophthalmology & optometry, visual_art.visual_art_medium, sense organs, 030217 neurology & neurosurgery, gp100 Melanoma Antigen |
| الوصف: | Purpose Maintaining mature and viable retinal pigment epithelial cells (RPE) in vitro has proven challenging. Investigating compounds that can promote RPE-viability and maturation is motivated by RPE transplantation research, the quest to understand RPE physiology, and a desire to modulate RPE in pathological states. We have previously reported that the silk protein sericin promotes viability, maturation, and pigmentation of human fetal RPE. In the present study, our aim was to uncover whether these effects can be seen in adult retinal pigment epithelial cell line-19 (ARPE-19) and induced pluripotent stem cell-derived RPE (iPSC-RPE). Methods ARPE-19 and iPSC-RPE were cultured with or without 10 mg/mL sericin. After 7 days, viability was assessed with calcein-acetoxymethyl ester (CAM) and ethidium homodimer-1 (EH-1) assays, flow cytometry, and morphometric analysis. Expression levels of RPE65, tyrosinase, and Pmel17 were quantified to compare maturation between the sericin-treated and control cultures. Light microscopy and staining of the tight junction protein zonula occludens protein 1 (ZO-1) were employed to study sericin’s effects on RPE morphology. We also measured culture medium pH, glucose, lactate, and extracellular ion content. Results Sericin-supplemented RPE cultures demonstrated significantly better viability compared to control cultures. Sericin appeared to improve ARPE-19 maturation and morphology in vitro. No effects were seen on RPE pigmentation with the concentration of sericin and duration of cell culture herein reported. Conclusions This is the first study to demonstrate that supplementing the culture media with sericin promotes the viability of iPSC-RPE and ARPE-19. Sericin’s viability-promoting effects may have important implications for retinal therapeutics and regenerative medicine research. |
| تدمد: | 1460-2202 0271-3683 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::536ebad30d32c197c1e99bcdabe2f7aeTest https://doi.org/10.1080/02713683.2020.1809001Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....536ebad30d32c197c1e99bcdabe2f7ae |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602202 02713683 |
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