Assay of DNA Photolyase Activity in Spinach Leaves in Relation to Cell Compartmentation-Evidence for Lack of DNA Photolyase in Chloroplasts
| العنوان: | Assay of DNA Photolyase Activity in Spinach Leaves in Relation to Cell Compartmentation-Evidence for Lack of DNA Photolyase in Chloroplasts |
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| المؤلفون: | Jorn Goss, Megumi Hada, Masateru Shin, Keisuke Hino, Gunther Buchholz, Eckard Wellmann |
| المصدر: | Bioscience, Biotechnology, and Biochemistry. 64:1288-1291 |
| بيانات النشر: | Informa UK Limited, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Chloroplasts, DNA Repair, DNA repair, education, Enzyme-Linked Immunosorbent Assay, Pyrimidine dimer, Applied Microbiology and Biotechnology, Biochemistry, Analytical Chemistry, Deoxyribodipyrimidine photo-lyase, Mice, chemistry.chemical_compound, Spinacia oleracea, Animals, Photolyase, Molecular Biology, biology, Organic Chemistry, Antibodies, Monoclonal, food and beverages, General Medicine, DNA photolyase, biology.organism_classification, Molecular biology, Cell Compartmentation, Plant Leaves, chemistry, biology.protein, Spinach, Deoxyribodipyrimidine Photo-Lyase, DNA, Biotechnology, Peroxidase |
| الوصف: | Spinach cyclobutane pyrimidine dimer (CPD)-specific DNA photolyase was successfully detected in leaf extracts by an assay system for plant photolyase using an improved enzyme-linked immunosorbent assay (ELISA) which was newly introduced by novel horseradish peroxidase (HRP)-linked CPD specific monoclonal antibodies. The assay system includes two main steps: a photorepair reaction of CPD introduced in substrate DNA and measurement of CPD remained after the photorepair by the improved ELISA. When CPD- induced salmon sperm DNA was used as a substrate, high CPD-photolyase activities were observed in the enzyme fraction prepared from whole spinach leaf extracts, but not from chloroplast extracts. This strongly suggests that spinach CPD-specific photolyases are localized in cell compartments other than chloroplasts. |
| تدمد: | 1347-6947 0916-8451 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2b4a2af3a5266e1811ea7768fdeff9fTest https://doi.org/10.1271/bbb.64.1288Test |
| رقم الانضمام: | edsair.doi.dedup.....d2b4a2af3a5266e1811ea7768fdeff9f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13476947 09168451 |
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