المؤلفون: Alain Pugin, Adrien Gauthier, David Wendehenne, Olivier Lamotte, David Reboutier, François Bouteau

المساهمون: ProdInra, Migration, Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Laboratoire Interdisciplinaire des Energies de Demain (LIED (UMR_8236)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD), Universite de Fribourg, Laboratoire d'Electrophysiologie des Membranes (LEM) EA 3514, Université Paris Diderot - Paris 7 (UPD7), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )

المصدر: Plant Signaling and Behavior
Plant Signaling and Behavior, Taylor & Francis, 2014, 2 (2), pp.86-95. ⟨10.4161/psb.2.2.4015⟩
Plant Signaling and Behavior, Taylor & Francis, 2007, 2 (2), pp.89-98
HAL
Plant Signaling and Behavior, 2014, 2 (2), pp.86-95. ⟨10.4161/psb.2.2.4015⟩

مصطلحات موضوعية: Hypersensitive response, Programmed cell death, [SDV]Life Sciences [q-bio], chemistry.chemical_element, Plant Science, Biology, Calcium, CELL DEATH, CALCIUM, [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics, [SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, PLASMA MEMBRANE DEPOLARIZATION, ComputingMilieux_MISCELLANEOUS, CRYPTOGEIN, food and beverages, Depolarization, Plant cell, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, Elicitor, Cell biology, PROTEASES, Electrophysiology, chemistry, Biochemistry, Efflux, Research Paper, ANION CHANNELS

الوصف: Anion effluxes are amongst the earliest reactions of plant cells to elicitors of defence responses. However, their properties and their role in disease resistance remain almost unknown. We previously demonstrated that cryptogein, an elicitor of tobacco defence responses, induces a nitrate (NO(3) (-)) efflux. This efflux is an early prerequisite to the cryptogein-triggered hypersensitive response (HR). Here, we analyzed the electrophysiological properties of the elicitor-mediated NO(3) (-) efflux and clarified the mechanisms through which it contributes to cell death. Application of the discontinuous single electrode voltage-clamp technique in tobacco cells elicited with cryptogein enabled us to record the activation of slow-type deactivating anion channel currents. Cryptogein-induced plasma membrane depolarization and Ca(2+) influx, an essential component of elicitor signalling for HR cell death, were prevented by inhibiting the NO(3) (-) efflux. Similarly, pharmacological blocking of the anion efflux suppressed vacuolar collapse, a hallmark of cell death. The role of NO(3) (-) efflux in mediating proteases activation was further assessed. It is shown that cryptogein induced the activation of three proteases with apparent molecular masses of 95, 190 and 240 kDa. Their activation occurred independently on the anion efflux and, together with cell death, was strongly reduced by cycloheximide and the protease inhibitor PMSF. In contrast, the NO(3) (-) efflux was shown to promote the accumulation of transcripts encoding vacuolar processing enzymes, a family of proteases previously reported to contribute to the disruption of vacuole integrity observed during the HR. Collectively, our data indicate that anion efflux is an early prerequisite to morphological and biochemical events participating to cell death.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6333cc7e6b3d6e9616802a96652f8702Test
https://doi.org/10.4161/psb.2.2.4015Test

المؤلفون: Jennifer Dahan, Valentin Hammoudi, David Wendehenne, Stéphane Bourque

المساهمون: Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)

المصدر: Plant Signaling and Behavior
Plant Signaling and Behavior, Taylor & Francis, 2011, 6 (11), pp.1865-1867. ⟨10.4161/psb.6.11.17848⟩

مصطلحات موضوعية: 0106 biological sciences, Hypersensitive response, Programmed cell death, [SDV]Life Sciences [q-bio], Histone Deacetylase 2, Plant Science, Models, Biological, 01 natural sciences, 03 medical and health sciences, Gene Expression Regulation, Plant, HDAC, Plant Cells, Tobacco, Nuclear protein, Plant Proteins, acetylation, 030304 developmental biology, 0303 health sciences, elicitor, biology, phosphorylation, Histone deacetylase 2, nucleus, food and beverages, protein kinase, Addendum, Elicitor, Cell biology, cell death, Histone, biology.protein, Histone deacetylase, Signal transduction, Signal Transduction, 010606 plant biology & botany

الوصف: article addendum : Bourque S, Dutartre A, Hammoudi V, Blanc S, Dahan J, Jeandroz S, Pichereaux C, Rossignol M, Wendehenne D., 2011. Type-2 histone deacetylases as new regulators of elicitorinduced cell death in plants. New Phytologist, 192 (1), 127–139. DOI: 10.1111/j.1469-8137.2011.03788.x; International audience; The cell death which characterizes the onset of the Hypersensitive Response (HR) is a very important weapon evolved by plants to block pathogen development. By the use of numerous plant/avirulent pathogen or plant/elicitor models, we have now obtained detailed signalling pathways allowing, after pathogen or elicitor perception, the control of the expression of specific sets of genes that contribute to cell death. However, our knowledge of the molecular actors involved in this process still remains limited. This is particularly true when regarding what happen in the nucleus. We recently reported that nuclear post-translational protein modifications are major processes that control cell death. Using the tobacco/cryptogein model, we showed that type 2 histone deacetylase activities, which act as negative regulators of cell death, depend on their phosphorylation status. In the present paper, we integrated all these results to propose a model depicting the putative nuclear signalling pathways controlling the establishment of cell death in tobacco in response to the cryptogein elicitor. This model highlights the role of the nuclear protein acetylation and phosphorylation in the establishment of plant defences.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7d18dd7a45a0e6efe6edadb9f380874Test
https://doi.org/10.4161/psb.6.11.17848Test