Salmonellaghosts expressing enterotoxigenicEscherichia colik88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model
| العنوان: | Salmonellaghosts expressing enterotoxigenicEscherichia colik88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model |
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| المؤلفون: | Seong Kug Eo, Chan Song Kim, Jin Hur, John Hwa Lee, Sang-Youel Park |
| المصدر: | Veterinary Quarterly. 35:125-132 |
| بيانات النشر: | Informa UK Limited, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Salmonella, animal diseases, medicine.medical_treatment, Bacterial Toxins, Blotting, Western, Biology, medicine.disease_cause, Microbiology, Mice, Immune system, Antigen, Enterotoxigenic Escherichia coli, medicine, Animals, Immunity, Mucosal, Escherichia coli Infections, Feces, DNA Primers, Adhesins, Escherichia coli, Antigens, Bacterial, Mice, Inbred BALB C, General Veterinary, Escherichia coli Proteins, Vaccination, Histocompatibility Antigens Class II, Antibodies, Bacterial, Virology, Immunoglobulin A, Disease Models, Animal, Diarrhea, Antigens, Surface, Bacterial Vaccines, bacteria, Female, Fimbriae Proteins, medicine.symptom, Adjuvant |
| الوصف: | Bacterial ghosts can be developed as safe and effective vaccines against bacterial infectious disease such as enterotoxigenic Escherichia coli (ETEC)-induced diarrhea in neonatal piglets.Immune responses against a Salmonella ghost expressing ETEC K88ab, K88ac, K99, and FasA antigens with various adjuvants and inoculation routes were evaluated in mice.A ghost cell expressing K88ab, K88ac, K99, and FasA fimbrial antigens of ETEC on the envelope of △asd Salmonella typhimurium was constructed as a candidate vaccine against ETEC infection. To optimize the immunization strategy, 6-week-old female BALB/c mice were inoculated with the ghost and various adjuvants, and the immune responses against the individual fimbrial antigens were measured. Blood samples from caudal vein to evaluate serum IgG concentrations and fecal samples to evaluate mucosal IgA concentrations were collected up to 14 weeks post-prime immunization.All groups with single, double, and triple inoculations of the ghost showed higher humoral and mucosal immune responses than the control group. In particular, the groups with intramuscular double and triple inoculations showed significantly higher immune responses. In addition, oral inoculation with a combination of the ghost and MONTANIDE IMS 1113 (MI1113) resulted in high and prolonged induction of intestinal IgA levels.These results indicated that both systemic and mucosal immunity against ETEC fimbrial antigens expressed by the ghost are induced by intramuscular booster inoculation with the ghost, and that addition of M1113 to the ghost was found to result in prominent induction of mucosal immunity through oral inoculation. |
| تدمد: | 1875-5941 0165-2176 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9c8f32b884c55c6cf0e6221d5c068d8Test https://doi.org/10.1080/01652176.2015.1029598Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b9c8f32b884c55c6cf0e6221d5c068d8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18755941 01652176 |
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