Cryptogein-Induced Anion Effluxes
| العنوان: | Cryptogein-Induced Anion Effluxes |
|---|---|
| المؤلفون: | Alain Pugin, Adrien Gauthier, David Wendehenne, Olivier Lamotte, David Reboutier, François Bouteau |
| المساهمون: | ProdInra, Migration, Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Laboratoire Interdisciplinaire des Energies de Demain (LIED (UMR_8236)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD), Universite de Fribourg, Laboratoire d'Electrophysiologie des Membranes (LEM) EA 3514, Université Paris Diderot - Paris 7 (UPD7), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) |
| المصدر: | Plant Signaling and Behavior Plant Signaling and Behavior, Taylor & Francis, 2014, 2 (2), pp.86-95. ⟨10.4161/psb.2.2.4015⟩ Plant Signaling and Behavior, Taylor & Francis, 2007, 2 (2), pp.89-98 HAL Plant Signaling and Behavior, 2014, 2 (2), pp.86-95. ⟨10.4161/psb.2.2.4015⟩ |
| بيانات النشر: | Informa UK Limited, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Hypersensitive response, Programmed cell death, [SDV]Life Sciences [q-bio], chemistry.chemical_element, Plant Science, Biology, Calcium, CELL DEATH, CALCIUM, [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics, [SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, PLASMA MEMBRANE DEPOLARIZATION, ComputingMilieux_MISCELLANEOUS, CRYPTOGEIN, food and beverages, Depolarization, Plant cell, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, Elicitor, Cell biology, PROTEASES, Electrophysiology, chemistry, Biochemistry, Efflux, Research Paper, ANION CHANNELS |
| الوصف: | Anion effluxes are amongst the earliest reactions of plant cells to elicitors of defence responses. However, their properties and their role in disease resistance remain almost unknown. We previously demonstrated that cryptogein, an elicitor of tobacco defence responses, induces a nitrate (NO(3) (-)) efflux. This efflux is an early prerequisite to the cryptogein-triggered hypersensitive response (HR). Here, we analyzed the electrophysiological properties of the elicitor-mediated NO(3) (-) efflux and clarified the mechanisms through which it contributes to cell death. Application of the discontinuous single electrode voltage-clamp technique in tobacco cells elicited with cryptogein enabled us to record the activation of slow-type deactivating anion channel currents. Cryptogein-induced plasma membrane depolarization and Ca(2+) influx, an essential component of elicitor signalling for HR cell death, were prevented by inhibiting the NO(3) (-) efflux. Similarly, pharmacological blocking of the anion efflux suppressed vacuolar collapse, a hallmark of cell death. The role of NO(3) (-) efflux in mediating proteases activation was further assessed. It is shown that cryptogein induced the activation of three proteases with apparent molecular masses of 95, 190 and 240 kDa. Their activation occurred independently on the anion efflux and, together with cell death, was strongly reduced by cycloheximide and the protease inhibitor PMSF. In contrast, the NO(3) (-) efflux was shown to promote the accumulation of transcripts encoding vacuolar processing enzymes, a family of proteases previously reported to contribute to the disruption of vacuole integrity observed during the HR. Collectively, our data indicate that anion efflux is an early prerequisite to morphological and biochemical events participating to cell death. |
| تدمد: | 1559-2324 1559-2316 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6333cc7e6b3d6e9616802a96652f8702Test https://doi.org/10.4161/psb.2.2.4015Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6333cc7e6b3d6e9616802a96652f8702 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15592324 15592316 |
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