Ceramide triggers metacaspase-independent mitochondrial cell death in yeast
| العنوان: | Ceramide triggers metacaspase-independent mitochondrial cell death in yeast |
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| المؤلفون: | Sabrina Büttner, Guido Kroemer, Frank Madeo, Ralf J. Braun, Didac Carmona-Gutierrez, Angela Reisenbichler, Kai-Uwe Fröhlich, Christoph Ruckenstuhl, Petra Heimbucher, Tobias Eisenberg, Patrick Rockenfeller, Maria A. Bauer |
| المصدر: | Cell Cycle. 10:3973-3978 |
| بيانات النشر: | Informa UK Limited, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Programmed cell death, Ceramide, Saccharomyces cerevisiae Proteins, Saccharomyces cerevisiae, Apoptosis, Biology, Ceramides, Necrosis, chemistry.chemical_compound, Molecular Biology, Cell Proliferation, chemistry.chemical_classification, Reactive oxygen species, Cell Biology, biology.organism_classification, Yeast, Metacaspase, Mitochondria, Cell biology, Biochemistry, chemistry, Caspases, Fermentation, Genome, Mitochondrial, Cancer cell, Reactive Oxygen Species, Developmental Biology |
| الوصف: | The activation of ceramide-generating enzymes, the blockade of ceramide degradation, or the addition of ceramide analogues can trigger apoptosis or necrosis in human cancer cells. Moreover, endogenous ceramide plays a decisive role in the killing of neoplastic cells by conventional anticancer chemotherapeutics. Here, we explored the possibility that membrane-permeable C2-ceramide might kill budding yeast (Saccharomyces cerevisiae) cells under fermentative conditions, where they exhibit rapid proliferation and a Warburg-like metabolism that is reminiscent of cancer cells. C2-ceramide efficiently induced the generation of reactive oxygen species (ROS), as well as apoptotic and necrotic cell death, and this effect was not influenced by deletion of the sole yeast metacaspase. However, C2-ceramide largely failed to cause ROS hypergeneration and cell death upon deletion of the mitochondrial genome. Thus, mitochondrial function is strictly required for C2-ceramide-induced yeast lethality. Accordingly, mitochondria from C2-ceramide-treated yeast cells exhibited major morphological alterations including organelle fragmentation and aggregation. Altogether, our results point to a pivotal role of mitochondria in ceramide-induced yeast cell death. |
| تدمد: | 1551-4005 1538-4101 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93df12c7632debeb30d870e2b942bca7Test https://doi.org/10.4161/cc.10.22.18212Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....93df12c7632debeb30d870e2b942bca7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15514005 15384101 |
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