Profiling of hepatocellular proteins by 1D PAGE-MALDI/MS/MS in a rat heat stress model
| العنوان: | Profiling of hepatocellular proteins by 1D PAGE-MALDI/MS/MS in a rat heat stress model |
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| المؤلفون: | Koon Wui Lee, Pachiappan Arjunan, Shabbir Moochhala, Ganapathy Rajaseger, Lu Jia, Chin Leong Lim |
| المصدر: | Frontiers in Bioscience. 11:2924 |
| بيانات النشر: | IMR Press, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Male, Chemistry, Maldi ms ms, Dehydrogenase, Isomerase, Transketolase, Heat Stress Disorders, Sensitivity and Specificity, Rats, Heat stress, Molecular Weight, Liver, Biochemistry, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Animals, Fumarylacetoacetate hydrolase, Electrophoresis, Polyacrylamide Gel, Rats, Wistar, Polyacrylamide gel electrophoresis, Heat-Shock Proteins, Pyruvate kinase |
| الوصف: | Heat induced complications cause an increase in a large number of proteins which play a role in diverse pathways during heat shock. A detailed characterization of these proteins is essential for understanding the molecular mechanisms involved in heat stroke. In this report, the proteins present in rat liver were compared at 37 degrees C (control) and at core temperature (Tc) 42 degrees C (heat stress) by 1D PAGE and MALDI/MS/MS. Among proteins identified in the sample after heat stress are dimethyglycine dehydrogenase, transketolase, carboxylic ester hydrolase, pyruvate kinase, L-type pyruvate kinase, arginosuccinate synthetase; fumarylacetoacetate hydrolase and peptidylpropyl isomerase A. These findings show that analysis of large scale proteins by MALDI/MS/MS provides a better understanding of the molecular mechanisms associated with heat shock. The resolution of proteins examined by 1D-PAGE was less than that obtained with 2D-PAGE. More specifically, 2D-PAGE allows better identification of low molecular weight proteins that can not be resolved by 1D-PAGE. |
| تدمد: | 1093-4715 1093-9946 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af36d7940e09b3255d50279f2063110aTest https://doi.org/10.2741/2021Test |
| رقم الانضمام: | edsair.doi.dedup.....af36d7940e09b3255d50279f2063110a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10934715 10939946 |
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