Prospective validation of microRNA signatures for detecting pancreatic malignant transformation in endoscopic-ultrasound guided fine-needle aspiration biopsies
| العنوان: | Prospective validation of microRNA signatures for detecting pancreatic malignant transformation in endoscopic-ultrasound guided fine-needle aspiration biopsies |
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| المؤلفون: | Nagy A. Habib, Niccola Funel, Nima Abbassi-Ghadi, Panagiotis Vlavianos, Leandro Castellano, Giovanna Del Vecchio Blanco, Elisa Giovannetti, Tamara M H Gall, Justin Stebbing, Jonathan Krell, Harry Kaltsidis, Long R. Jiao, Mireia Mato Prado, Adam E Frampton, Mohamed Basyouny |
| المساهمون: | The Alliance Family Foundation, Medical oncology laboratory, CCA - Cancer biology |
| المصدر: | Frampton, A E, Krell, J, Prado, M M, Gall, T M H, Abbassi-Ghadi, N, Blanco, G D V, Funel, N, Giovannetti, E, Castellano, L, Basyouny, M, Habib, N A, Kaltsidis, H, Vlavianos, P, Stebbing, J & Jiao, L R 2016, ' Prospective validation of microRNA signatures for detecting pancreatic malignant transformation in endoscopic-ultrasound guided fine-needle aspiration biopsies ', Oncotarget, vol. 7, no. 19, pp. 28556-28569 . https://doi.org/10.18632/oncotarget.8699Test Oncotarget, 7(19), 28556-28569. Impact Journals Oncotarget Oncotarget 7 (2016): 28556–28569. doi:10.18632/oncotarget.8699 info:cnr-pdr/source/autori:Frampton A.E.; Krell J.; Mato Prado M.; Gall T.M.H.; Abbassi-Ghadi N.; Del Vecchio Blanco G.; Funel N.; Giovannetti E.; Castellano L.; Basyouny M.; Habib N.A.; Kaltsidis H.; Vlavianos P.; Stebbing J.; Jiao L.R./titolo:Prospective validation of microRNA signatures for detecting pancreatic malignant transformation in endoscopic-ultrasound guided fine-needle aspiration biopsies/doi:10.18632%2Foncotarget.8699/rivista:Oncotarget/anno:2016/pagina_da:28556/pagina_a:28569/intervallo_pagine:28556–28569/volume:7 |
| بيانات النشر: | Impact Journals, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Endoscopic ultrasound, Male, Disease, Malignant transformation, CYST FLUID, Settore MED/12, 0302 clinical medicine, Prospective Studies, Prospective cohort study, fine-needle aspiration (FNA), medicine.diagnostic_test, microRNA, Middle Aged, CANCER, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, Fine-needle aspiration, Cell Transformation, Neoplastic, Oncology, 030220 oncology & carcinogenesis, SURVIVAL, 030211 gastroenterology & hepatology, Female, Pancreas, Life Sciences & Biomedicine, Research Paper, NEOPLASMS, Adult, EXPRESSION, medicine.medical_specialty, pancreatic ductal adenocarcinoma, Translational research, DUCTAL ADENOCARCINOMA, Sensitivity and Specificity, 03 medical and health sciences, medicine, MANAGEMENT, Humans, Clinical significance, CLINICAL-SIGNIFICANCE, Endoscopic Ultrasound-Guided Fine Needle Aspiration, PREOPERATIVE DIAGNOSIS, Aged, Science & Technology, LESIONS, business.industry, General surgery, Gene Expression Profiling, endoscopic ultrasound (EUS), pancreatic cyst, Cell Biology, Surgery, Pancreatic Neoplasms, MicroRNAs, Logistic Models, business |
| الوصف: | // Adam E. Frampton 1, 2, * , Jonathan Krell 2, * , Mireia Mato Prado 2 , Tamara M.H. Gall 1 , Nima Abbassi-Ghadi 3 , Giovanna Del Vecchio Blanco 4 , Niccola Funel 5, 6 , Elisa Giovannetti 5, 6, 7 , Leandro Castellano 2 , Mohamed Basyouny 1 , Nagy A. Habib 1 , Harry Kaltsidis 8 , Panagiotis Vlavianos 8 , Justin Stebbing 2, # , Long R. Jiao 1, # 1 HPB Surgical Unit, Department of Surgery and Cancer, Imperial College, Hammersmith Hospital, London, UK 2 Division of Cancer, Department of Surgery and Cancer, Imperial College, Hammersmith Hospital, London, UK 3 Academic Surgical Unit, Department of Surgery and Cancer, Imperial College, St. Mary's Hospital, London, UK 4 Department of Systems Medicine, Gastroenterology Unit, University Tor Vergata, Rome, Italy 5 Cancer Pharmacology Lab, AIRC Start-Up Unit, Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy 6 CNR-Nano, Institute of Nanoscience and Nanotechnology, Pisa, Italy 7 Department of Medical Oncology, VU University Medical Center, Amsterdam, Netherlands 8 Department of Gastroenterology, Imperial College Healthcare NHS Trust, Hammersmith Hospital, London, UK * These authors share co-first authorship # These authors share co-senior authorship Correspondence to: Long R. Jiao, e-mail: l.jiao@imperial.ac.uk Keywords: microRNA, endoscopic ultrasound (EUS), fine-needle aspiration (FNA), pancreatic ductal adenocarcinoma, pancreatic cyst Received: August 01, 2015 Accepted: March 22, 2016 Published: April 12, 2016 ABSTRACT Background: Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease. Novel biomarkers are required to aid treatment decisions and improve patient outcomes. MicroRNAs (miRNAs) are potentially ideal diagnostic biomarkers, as they are stable molecules, and tumour and tissue specific. Results: Logistic regression analysis revealed an endoscopic-ultrasound fine-needle aspiration (EUS-FNA) 2-miRNA classifier (miR-21 + miR-155) capable of distinguishing benign from malignant pancreatic lesions with a sensitivity of 81.5% and a specificity of 85.7% (AUC 0.930). Validation FNA cohorts confirmed both miRNAs were overexpressed in malignant disease, while circulating miRNAs performed poorly. Methods: Fifty-five patients with a suspicious pancreatic lesion on cross-sectional imaging were evaluated by EUS-FNA. At echo-endoscopy, the first part of the FNA was sent for cytological assessment and the second part was used for total RNA extraction. Candidate miRNAs were selected after careful review of the literature and expression was quantified by qRT-PCR. Validation was performed on an independent cohort of EUS-FNAs, as well as formalin-fixed paraffin embedded (FFPE) and plasma samples. Conclusions: We provide further evidence for using miRNAs as diagnostic biomarkers for pancreatic malignancy. We demonstrate the feasibility of using fresh EUS-FNAs to establish miRNA-based signatures unique to pancreatic malignant transformation and the potential to enhance risk stratification and selection for surgery. |
| تدمد: | 1949-2553 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::461cf973d8440f8077c2adce10de847bTest http://hdl.handle.net/10044/1/37525Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....461cf973d8440f8077c2adce10de847b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19492553 |
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