Deuterated Peptides and Proteins: Structure and Dynamics Studies by MAS Solid-State NMR
| العنوان: | Deuterated Peptides and Proteins: Structure and Dynamics Studies by MAS Solid-State NMR |
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| المؤلفون: | Bernd Reif |
| المصدر: | Methods in Molecular Biology ISBN: 9781617794797 eMagRes |
| بيانات النشر: | Humana Press, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Deuterium NMR, Crystallography, Microcrystalline, Protein structure, Solid-state nuclear magnetic resonance, Deuterium, Carbon-13 NMR satellite, Chemistry, Protein dynamics, Protonation |
| الوصف: | Perdeuteration and back substitution of exchangeable protons in microcrystalline proteins, in combination with recrystallization from D(2)O-containing buffers, significantly reduce (1)H, (1)H dipolar interactions. This way, amide proton line widths on the order of 20 Hz are obtained. Aliphatic protons are accessible either via specifically protonated precursors or by using low amounts of H(2)O in the bacterial growth medium. The labeling scheme enables characterization of structure and dynamics in the solid-state without dipolar truncation artifacts. |
| ردمك: | 978-1-61779-479-7 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::48e12e23a0407870fd725a3e63ca01c7Test https://doi.org/10.1007/978-1-61779-480-3_16Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........48e12e23a0407870fd725a3e63ca01c7 |
| قاعدة البيانات: | OpenAIRE |
| ردمك: | 9781617794797 |
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