Rapid miRNA Imaging in Cells Using Fluorogenic Templated Staudinger Reaction Between PNA-Based Probes
| العنوان: | Rapid miRNA Imaging in Cells Using Fluorogenic Templated Staudinger Reaction Between PNA-Based Probes |
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| المؤلفون: | Nicolas Winssinger, Katarzyna Gorska |
| المصدر: | Peptide Nucleic Acids ISBN: 9781627035521 Peptide Nucleic Acids : Methods and Protocols Methods in Molecular Biology Peptide Nucleic Acids : Methods and Protocols pp. 197-192 |
| بيانات النشر: | Humana Press, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Fluorophore, Biocompatibility, Nucleic acid sequence, Peptide, Peptide nucleic acid (PNA), Fluorescence, Imaging, 3. Good health, chemistry.chemical_compound, chemistry, Biochemistry, Molecular beacon, Nucleic acid, Templated reaction, Pro-fluorophore, Staudinger reaction, Rhodamine, MiRNA |
| الوصف: | Reactions templated by a specific nucleic acid sequence have emerged as an attractive strategy for nucleic acid sensing. The Staudinger reaction using an azide-quenched fluorophore and a phosphine is particularly well suited by virtue of its bioorthogonality and biocompatibility. The reaction is promoted by a complementary nucleic acid that aligns the phosphine with the azide-quenched fluorophore. Cellular RNAs can catalyze the Staudinger reaction and signal amplification can be achieved through multiple turnover of the template. Peptide nucleic acids (PNA) provide a convenient platform for the preparation of specific probes as they combine desirable hybridization properties, robust synthesis, ease of fluorophore conjugation, and high biochemical stability. Herein, we describe protocols for fast fluorescent detection of miRNAs in human cells with PNA-based probes via reductive unquenching of bis-azidorhodamine by trialkylphosphine. |
| ردمك: | 978-1-62703-552-1 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::086ea8f7f53e7ddc40a96b4e7683648dTest https://doi.org/10.1007/978-1-62703-553-8_15Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....086ea8f7f53e7ddc40a96b4e7683648d |
| قاعدة البيانات: | OpenAIRE |
| ردمك: | 9781627035521 |
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