دورية أكاديمية
Neuropathology in 2 cases of fatal enterovirus type 71 infection from a recent epidemic in the People's Republic of China: a histopathologic, immunohistochemical, and reverse transcription polymerase chain reaction study
| العنوان: | Neuropathology in 2 cases of fatal enterovirus type 71 infection from a recent epidemic in the People's Republic of China: a histopathologic, immunohistochemical, and reverse transcription polymerase chain reaction study |
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| المؤلفون: | Yang, Yu, Wang, Hua, Gong, Encong, Du, Juan, Zhao, Xishun, McNutt, Michael A., Wang, Shenglan, Zhong, Yanfeng, Gao, Zifen, Zheng, Jie |
| المساهمون: | Gao, ZF (reprint author), Peking Univ, Beijing Autopsy Ctr, Dept Pathol, Hlth Sci Ctr, Beijing 100083, Peoples R China., Peking Univ, Beijing Autopsy Ctr, Dept Pathol, Hlth Sci Ctr, Beijing 100083, Peoples R China. |
| المصدر: | PubMed ; SCI |
| بيانات النشر: | human pathology |
| سنة النشر: | 2009 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | Enterovirus type 71, Reverse transcription polymerase chain reaction, Immunohistochemistry, Hand, foot, and mouth disease, MOUTH-DISEASE OUTBREAK, CENTRAL-NERVOUS-SYSTEM, PULMONARY-EDEMA, LISTERIA-MONOCYTOGENES, BRAIN-STEM, TAIWAN, ENCEPHALOMYELITIS, CHILDREN |
| الوصف: | Between late March and June of 2008, an outbreak of hand, foot, and mouth disease caused by enterovirus 71 Occurred in China. In this outbreak, more than 176 000 cases occurred, and at least 40 people died. However, there has been no description of the neuropathologic features of fatal enterovirus 71 infection cases during this outbreak. We report postmortem studies in 2 fatal cases of enterovirus 7 1 infection with examination of the central nervous system using histopathology, immunohistochemistry, and reverse transcription polymerase chain reaction. Characteristic features of acute encephalitis were found predominantly in the brain stem and spinal cord. Abundant numbers of inflammatory cells with unusual irregularly rod-shaped and lobulated nuclei, which mimicked neutrophil morphology, were found both in abscess-like clusters associated with necrosis, as well as in inflammatory infiltrates. immunohistochemistry showed that most of these cells were CD68 positive and CD15 negative. Viral antigens were found in the cytoplasm of neurons, neuronal processes, and inflammatory cells, most often associated with glial nodules. The presence of enterovirus 71 was confirmed by reverse transcription polymerase chain reaction and sequencing. Results indicate that immunohistochemistry with CD68 and CD15 may offer some help in the differential diagnosis of brain stein encephalitis caused by enterovirus 71. Postmortem reverse transcription polymerase chain reaction from brain stein tissues, which have already undergone fixation and histologic processing, can be an important diagnostic tool, which may be of particular value in patients who may have been misdiagnosed clinically. (C) 2009 Elsevier Inc. All rights reserved. ; Pathology ; SCI(E) ; PubMed ; 24 ; ARTICLE ; 9 ; 1288-1295 ; 40 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 0046-8177 19386354 |
| العلاقة: | HUMAN PATHOLOGY.2009,40,(9),1288-1295.; 671557; http://hdl.handle.net/20.500.11897/197616Test; WOS:000269368800009 |
| DOI: | 10.1016/j.humpath.2009.01.015 |
| الإتاحة: | https://doi.org/20.500.11897/197616Test https://doi.org/10.1016/j.humpath.2009.01.015Test https://hdl.handle.net/20.500.11897/197616Test |
| رقم الانضمام: | edsbas.D88E6356 |
| قاعدة البيانات: | BASE |
| تدمد: | 00468177 19386354 |
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| DOI: | 10.1016/j.humpath.2009.01.015 |