دورية أكاديمية
Immunohistochemical Expression of Somatostatin Receptor Subtypes in a Panel of Neuroendocrine Neoplasias
| العنوان: | Immunohistochemical Expression of Somatostatin Receptor Subtypes in a Panel of Neuroendocrine Neoplasias |
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| المؤلفون: | Remes, Satu M., Leijon, Helena L., Vesterinen, Tiina J., Arola, Johanna T., Haglund, Caj H. |
| المساهمون: | Department of Pathology, HUSLAB, Institute for Molecular Medicine Finland, Helsinki Institute of Life Science HiLIFE, University Management, HUS Abdominal Center, Department of Surgery, II kirurgian klinikka, CAN-PRO - Translational Cancer Medicine Program, Research Programs Unit, University of Helsinki |
| بيانات النشر: | HISTOCHEMICAL SOC INC |
| سنة النشر: | 2020 |
| المجموعة: | Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
| مصطلحات موضوعية: | immunohistochemistry, neuroendocrine tumor, somatostatin receptor, MONOCLONAL-ANTIBODIES, TISSUES, REASSESSMENT, VALIDATION, SSTR2A, TUMORS, 1182 Biochemistry, cell and molecular biology |
| الوصف: | Neuroendocrine neoplasias (NENs) are known to express somatostatin receptors (SSTRs) 1-5, which are G-protein-coupled cell membrane receptors. Somatostatin receptor imaging and therapy utilizes the SSTR expression. Synthetic somatostatin analogs with radioligands are used to detect primary tumors, metastases, and recurrent disease. Receptor analogs are also used for treating NENs. Furthermore, commercially available SSTR antibodies can be used for the immunohistochemical (IHC) detection of SSTRs. We investigated different SSTR antibody clones applying diverse IHC protocol settings to identify reliable clones and feasible protocols for NENs. A tissue microarray including NENs from 12 different primary sites were stained. Only UMB clones were able to localize SSTR on the cell membranes of NENs. SSTR2 (UMB1) emerged as the most common subtype followed by SSTR5 (UMB4) and SSTR1 (UMB7). SSTR3 (UMB5) expression was mainly cytoplasmic. Yet, SSTR4 expression was weak and located primarily in the cytoplasm. Thus, appropriate IHC protocols, including proper positive and negative controls, represent requirements for high-quality NEN diagnostics and for planning personalized therapy. ; Peer reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| ردمك: | 978-0-00-489131-6 0-00-489131-7 |
| العلاقة: | Remes , S M , Leijon , H L , Vesterinen , T J , Arola , J T & Haglund , C H 2019 , ' Immunohistochemical Expression of Somatostatin Receptor Subtypes in a Panel of Neuroendocrine Neoplasias ' , Journal of Histochemistry and Cytochemistry , vol. 67 , no. 10 , pp. 735-743 . https://doi.org/10.1369/0022155419856900Test; ORCID: /0000-0003-2997-0337/work/83840573; ddf78ade-41d0-4391-a39f-a6ae60d310e0; http://hdl.handle.net/10138/320964Test; 000489131700002 |
| الإتاحة: | http://hdl.handle.net/10138/320964Test |
| حقوق: | openAccess ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.C85AD473 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780004891316 0004891317 |
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