Herpesvirus BACs: Past, Present, and Future
| العنوان: | Herpesvirus BACs: Past, Present, and Future |
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| المؤلفون: | Qiyi Tang, Hua Zhu, Charles Warden |
| المصدر: | Journal of Biomedicine and Biotechnology BioMed Research International Journal of Biomedicine and Biotechnology, Vol 2011 (2011) |
| بيانات النشر: | Hindawi Publishing Corporation, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Chromosomes, Artificial, Bacterial, lcsh:Biotechnology, Health, Toxicology and Mutagenesis, viruses, DNA, Recombinant, lcsh:Medicine, Mutagenesis (molecular biology technique), Review Article, Biology, medicine.disease_cause, Genome, Herpesviridae, law.invention, 03 medical and health sciences, chemistry.chemical_compound, law, lcsh:TP248.13-248.65, Virology, Genetics, medicine, Cloning, Molecular, Molecular Biology, 030304 developmental biology, Cloning, 0303 health sciences, Bacterial artificial chromosome, 030306 microbiology, lcsh:R, General Medicine, 3. Good health, chemistry, Mutagenesis, Recombinant DNA, Molecular Medicine, Transposon mutagenesis, Corrigendum, DNA, Biotechnology |
| الوصف: | The herpesviridae are a large family of DNA viruses with large and complicated genomes. Genetic manipulation and the generation of recombinant viruses have been extremely difficult. However, herpesvirus bacterial artificial chromosomes (BACs) that were developed approximately 10 years ago have become useful and powerful genetic tools for generating recombinant viruses to study the biology and pathogenesis of herpesviruses. For example, BAC-directed deletion mutants are commonly used to determine the function and essentiality of viral genes. In this paper, we discuss the creation of herpesvirus BACs, functional analyses of herpesvirus mutants, and future applications for studies of herpesviruses. We describe commonly used methods to create and mutate herpesvirus BACs (such as site-directed mutagenesis and transposon mutagenesis). We also evaluate the potential future uses of viral BACs, including vaccine development and gene therapy. |
| اللغة: | English |
| تدمد: | 1110-7251 1110-7243 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa149f094123012ec2dd46903c2c845dTest http://europepmc.org/articles/PMC2965428Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fa149f094123012ec2dd46903c2c845d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 11107251 11107243 |
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