CD44 in Differentiated Embryonic Stem Cells: Surface Expression and Transcripts Encoding Multiple Variants
| العنوان: | CD44 in Differentiated Embryonic Stem Cells: Surface Expression and Transcripts Encoding Multiple Variants |
|---|---|
| المؤلفون: | Hélène Haegel, Andrée Dierich, Rhodri Ceredig |
| المصدر: | Developmental Immunology Clinical and Developmental Immunology, Vol 3, Iss 4, Pp 239-246 (1994) |
| بيانات النشر: | Hindawi Publishing Corporation, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | lcsh:Immunologic diseases. Allergy, Cellular differentiation, Immunology, Receptors, Lymphocyte Homing, Receptors, Cell Surface, Cell Line, Exon, Mice, Animals, adhesion molecules, Northern blot, RNA, Messenger, CD44, biology, Stem Cells, Alternative splicing, Cell Differentiation, embryonic stem cells, Molecular biology, Embryonic stem cell, Cell biology, Alternative Splicing, Hyaluronan Receptors, Cell culture, Antigens, Surface, biology.protein, Stem cell, lcsh:RC581-607, Carrier Proteins, Developmental Biology, Research Article |
| الوصف: | Expression of the surface-adhesion molecule CD44 was investigated during thein vitrodifferentiation of the embryonic stem (ES) cell line D3. By immunofluorescence analysis, totipotent, undifferentiated ES cells did not show surface expression of CD44, although two transcripts of approximately 1.6 and 3.3 kb were detected on Northern blots. Following 1 week of differentiation in either suspension or substrate-attached cultures, CD44 appeared on the surface of some D3 cells, and synthesis of an additional 4.5 kb mRNA species was detected on Northern blots. At this stage, at least three distinct transcripts encoding CD44 variants were induced within the cultures, resulting from alternative splicing of additional exons in the variable domains of CD44. From PCR analysis, they all appeared to contain the variable exon v10, and two of them in addition contained v6. Taken together, these results suggest that CD44 may play a role in cell migration and adhesion in the early development of the mouse embryo. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 1044-6672 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c9aa368d551d4a6298b12fc5fc22973Test http://europepmc.org/articles/PMC2275938Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2c9aa368d551d4a6298b12fc5fc22973 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10446672 |
|---|