دورية أكاديمية
Exploring T cell reactivity to gliadin in young children with newly-diagnosed Celiac disease
| العنوان: | Exploring T cell reactivity to gliadin in young children with newly-diagnosed Celiac disease |
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| المؤلفون: | Liu, Edwin, McDaniel, Kristen, Case, Stephanie, Yu, Liping, Gerhartz, Bernd, Ostermann, Nils, Fankhauser, Gabriela, Hungerford, Valerie, Zou, Chao, Luyten, Marcel, Seidl, Katherine, Michels, Aaron |
| بيانات النشر: | Hindawi Publishing Corporation |
| سنة النشر: | 2014 |
| المجموعة: | The Novartis Repository (Novartis, Switzerland) |
| الوصف: | Class II major histocompatibility molecules confer disease risk in Celiac disease (CD) by presenting gliadin peptides to CD4 T cells in the small intestine. Deamidation of gliadin peptides by tissue transglutaminase creates immunogenic peptides presented by HLA-DQ2 and DQ8 molecules to activate proinflammatory CD4 T cells. Detecting gliadin specific T cell responses from the peripheral blood has been challenging due to low circulating frequencies and heterogeneity in response to gliadin epitopes. We investigated the peripheral T cell responses to alpha and gamma gliadin epitopes in young children with newly diagnosed and untreated CD. Using peptide/MHC recombinant protein constructs, we are able to robustly stimulate CD4 T cell clones previously derived from intestinal biopsies of CD patients. These recombinant proteins and a panel of α- and γ-gliadin peptides were used to assess T cell responses from the peripheral blood. Proliferation assays using peripheral blood mononuclear cells revealed more CD4 T cell responses to α-gliadin than γ-gliadin peptides with a single deamidated α-gliadin peptide able to identify 60% of CD children. We conclude that α-gliadin epitopes are prevalent in newly diagnosed CD children which have important implications for monitoring disease activity. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | Liu, Edwin, McDaniel, Kristen, Case, Stephanie, Yu, Liping, Gerhartz, Bernd, Ostermann, Nils, Fankhauser, Gabriela, Hungerford, Valerie, Zou, Chao, Luyten, Marcel, Seidl, Katherine and Michels, Aaron (2014) Exploring T cell reactivity to gliadin in young children with newly-diagnosed Celiac disease. Autoimmune Diseases, 2014. pp. 1-8. ISSN 0076-6879 |
| DOI: | 10.1155/2014/927190 |
| الإتاحة: | https://doi.org/10.1155/2014/927190Test https://oak.novartis.com/20327Test/ http://www.ncbi.nlm.nih.gov/pubmed/24724018Test |
| رقم الانضمام: | edsbas.8B7CF40B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1155/2014/927190 |
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