دورية أكاديمية
Influence of Melatonin on Behavioral and Neurological Function of Rats with Focal Cerebral Ischemia-Reperfusion Injury via the JNK/FoxO3a/Bim Pathway
| العنوان: | Influence of Melatonin on Behavioral and Neurological Function of Rats with Focal Cerebral Ischemia-Reperfusion Injury via the JNK/FoxO3a/Bim Pathway |
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| المؤلفون: | Xingwang Chen, Xueyuan Shen, Jianbo Lai, Zhijun Yao, Xian Peng, Long Wu, Yuantong Ou, Huachu Wu, Haofeng Zhu, Yiyu Deng |
| المصدر: | Computational and Mathematical Methods in Medicine, Vol 2022 (2022) |
| بيانات النشر: | Hindawi Limited |
| سنة النشر: | 2022 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Computer applications to medicine. Medical informatics, R858-859.7 |
| الوصف: | Objective. To investigate the influence of melatonin on behavioral and neurological function of rats with focal cerebral ischemia-reperfusion injury via the JNK/FoxO3a/Bim pathway. Methods. One hundred and twenty healthy male SD rats were randomized into the model group (Model: the middle cerebral artery occlusion (MCAO) model was constructed and received an equal volume of normal saline containing 5% DMSO), sham operation group (Sham: received no treatment except normal feeding), and low, medium, and high dose of melatonin group (L-MT, M-MT, and H-MT intraperitoneally injected 10, 20, and 40 mg/kg melatonin 30 min after IR, respectively), with 24 rats in each group. Following 24 h of reperfusion, the rats in each of the above groups were tested for neurological deficit symptoms and behavioral changes to screen the rats included in the study. HE and TUNEL stainings were performed to observe pathological changes. Levels of oxidative stress-related indexes, inflammatory factor-related indexes, nuclear factor-κB p65 (NF-κB p65), and interferon-γ (IFN-γ) in the rat brain were measured by ELISA. The JNK/FoxO3a/Bim pathway-related proteins as well as Bcl-2, Caspase-3, and Bax were examined using Western blot. Results. Detection of behavioral indicators showed that the MACO model was successfully constructed in rats. L-MT, M-MT, and L-MT groups presented reduced malondialdehyde (MDA), reactive oxygen species (ROS), tumor necrosis factor- (TNF-) α, interleukin- (IL-) 6, IL-1β, IFN-γ, NF-κB p65, and apoptosis compared with the Model group (P<0.05), and the improvement degree was better in the M-MT group versus the L-HT group. Bcl-2 protein expression in the brain tissue of L-MT, M-MT, and H-MT groups increased significantly, while Bax, Caspase-3, p-JNK, p-FoxO3a, and Bim protein expression declined markedly, versus the Model group (P<0.05). The changes of indexes were greater in the M-MT group compared with that in the L-MT group. No significant difference was observed in all the above indexes between the M-MT ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1748-6718 |
| العلاقة: | http://dx.doi.org/10.1155/2022/8202975Test; https://doaj.org/toc/1748-6718Test; https://doaj.org/article/7d5f834ac06b46ddbd3657398aa11fdcTest |
| DOI: | 10.1155/2022/8202975 |
| الإتاحة: | https://doi.org/10.1155/2022/8202975Test https://doaj.org/article/7d5f834ac06b46ddbd3657398aa11fdcTest |
| رقم الانضمام: | edsbas.56D072F6 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 17486718 |
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| DOI: | 10.1155/2022/8202975 |