Brazilian Green Propolis: EffectsIn VitroandIn VivoonTrypanosoma cruzi
| العنوان: | Brazilian Green Propolis: EffectsIn VitroandIn VivoonTrypanosoma cruzi |
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| المؤلفون: | Andrea Henriques-Pons, Eniuce Menezes de Souza, Helene Santos Barbosa, Solange L. de Castro, Kelly Salomão |
| المصدر: | Evidence-Based Complementary and Alternative Medicine, Vol 2011 (2011) Evidence-based Complementary and Alternative Medicine : eCAM |
| بيانات النشر: | Hindawi Limited, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | biology, lcsh:Other systems of medicine, Parasitemia, Pharmacology, Propolis, lcsh:RZ201-999, medicine.disease, biology.organism_classification, In vitro, Complementary and alternative medicine, In vivo, parasitic diseases, Toxicity, Immunology, medicine, Original Article, Amastigote, Energy source, Trypanosoma cruzi |
| الوصف: | The composition of a Brazilian green propolis ethanolic extract (Et-Bra) and its effect onTrypanosoma cruzitrypomastigotes and other pathogenic microorganisms have already been reported. Here, we further investigated Et-Bra targets inT. cruziand its effect on experimental infection of mice. The IC50/4 days for inhibition of amastigote proliferation was 8.5 ± 1.8 μg mL−1, with no damage to the host cells. In epimastigotes Et-Bra induced alterations in reservosomes, Golgi complex and mitochondrion. These effects were confirmed by flow cytometry analysis. In trypomastigotes, Et-Bra led to the loss of plasma membrane integrity. Thein vitrostudies indicate that Et-Bra interferes in the functionality of the plasma membrane in trypomastigotes and of reservosomes and mitochondrion in epimastigotes. Acutely infected mice were treated orally with Et-Bra and the parasitemia, mortality and GPT, GOT, CK and urea levels were monitored. The extract (25–300 mg kg−1body weight/day for 10 days) reduced the parasitemia, although not at significant levels; increased the survival of the animals and did not induce any hepatic, muscular lesion or renal toxicity. Since Et-Bra was not toxic to the animals, it could be assayed in combination with other drugs. Et-Bra could be a potential metacyclogenesis blocker, considering its effect on reservosomes, which are an important energy source during parasite differentiation. |
| تدمد: | 1741-4288 1741-427X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08a65f3da77df2dd543bbf057858aae0Test https://doi.org/10.1093/ecam/nep014Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....08a65f3da77df2dd543bbf057858aae0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17414288 1741427X |
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