Low Level of Her-2 Locus Amplification by Fluorescent In Situ Hybridization Does Not Correlate with Her-2 Protein Overexpression by Immunohistochemistry in Barrett's Esophagus
| العنوان: | Low Level of Her-2 Locus Amplification by Fluorescent In Situ Hybridization Does Not Correlate with Her-2 Protein Overexpression by Immunohistochemistry in Barrett's Esophagus |
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| المؤلفون: | Agnieszka M. Rygiel, K. K. Krishnadath, Fiebo J.W. ten Kate, Francesca Milano, Jacques J. Bergman |
| المساهمون: | Center of Experimental and Molecular Medicine, Amsterdam Gastroenterology Endocrinology Metabolism, Cancer Center Amsterdam, Gastroenterology and Hepatology, Pathology |
| المصدر: | Journal of Oncology, Vol 2010 (2010) Journal of oncology, 2010. Hindawi Publishing Corporation Journal of Oncology |
| بيانات النشر: | Hindawi Limited, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | education.field_of_study, Pathology, medicine.medical_specialty, Article Subject, Population, Locus (genetics), In situ hybridization, Biology, medicine.disease, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Molecular biology, Fluorescence, lcsh:RC254-282, Chromosome 17 (human), Oncology, Barrett's esophagus, medicine, Clinical Study, Immunohistochemistry, HER-2 protein, education |
| الوصف: | An accurate evaluation of the Her-2 status has important prognostic and therapeutic implications in many carcinomas. The aim of the study was to correlate Her-2 locus (17q11.2) amplification and chromosome 17 gains as assessed by fluorescent in situ hybridization (FISH) with Her-2 protein overexpression by immunohistochemistry (IHC) in patients with Barrett's esophagus (BE) and esophageal adenocarcinoma (EAC). We analyzed 34 patients with Her-2 amplification and/or chromosome 17gains using FISH on brush cytology specimens. Seven patients (21%) showed high Her-2 locus amplification ( Her-2: Cep17 5 : 1), 5 (15%) showed low Her-2 locus amplification ( Her-2: Cep17 2 5 : 1), and 22 (65%) displayed gains of chromosome 17 only. Further, we confirmed Her-2 amplification on corresponding biopsies that were taken at the same occasion as the cytologybrushings. Then, we compared the FISH results with IHC data obtained from the corresponding biopsies and showed that low level of Her-2 amplification does not correlate with Her-2 protein overexpression (score / ; ), in contrast to the high amplification level ( ). Thus, in our population of BE and EAC patients, low level of Her-2 amplification does not result in detectable level of Her-2 protein as assessed by IHC. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 1687-8469 1687-8450 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5b19a7b746ffe81263ba8c34562f402Test https://doaj.org/article/c4158e6736a644ce964e4c36d6936d5eTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c5b19a7b746ffe81263ba8c34562f402 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16878469 16878450 |
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