المؤلفون: Christelle Darrieutort-Laffite, Marie-Astrid Boutet, Mathias Chatelais, Régis Brion, Frédéric Blanchard, Dominique Heymann, Benoit Le Goff

المصدر: Mediators of Inflammation, Vol 2014 (2014)

مصطلحات موضوعية: Pathology, RB1-214

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/0962-9351Test; https://doaj.org/toc/1466-1861Test

الوصول الحر: https://doaj.org/article/9e36fab9d15c42f3bc21b2580a2d08aeTest

المؤلفون: Régis Brion, Mathias Chatelais, Benoit Le Goff, Dominique Heymann, Marie-Astrid Boutet, Christelle Darrieutort-Laffite, Frédéric Blanchard

المصدر: Mediators of Inflammation
Mediators of Inflammation, Vol 2014 (2014)

مصطلحات موضوعية: Macrophage colony-stimulating factor, Article Subject, Cell Survival, CD14, Interleukin-1beta, Immunology, Arthritis, Monocytes, Arthritis, Rheumatoid, lcsh:Pathology, medicine, Humans, Cells, Cultured, Tumor Necrosis Factor-alpha, business.industry, Interleukins, Macrophage Colony-Stimulating Factor, Monocyte, Synovial Membrane, Granulocyte-Macrophage Colony-Stimulating Factor, Cell Biology, Fibroblasts, medicine.disease, medicine.anatomical_structure, Granulocyte macrophage colony-stimulating factor, Culture Media, Conditioned, Rheumatoid arthritis, Tumor necrosis factor alpha, Inflammation Mediators, Synovial membrane, business, lcsh:RB1-214, Research Article, medicine.drug

الوصف: Background. Macrophages and synovial fibroblasts (SF) are two major cells implicated in the pathogenesis of rheumatoid arthritis (RA). SF could be a source of cytokines and growth factors driving macrophages survival and activation. Here, we studied the effect of SF on monocyte viability and phenotype.Methods. SF were isolated from synovial tissue of RA patients and CD14+ cells were isolated from peripheral blood of healthy donors. SF conditioned media were collected after 24 hours of culture with or without stimulation with TNFαor IL-1β. Macrophages polarisation was studied by flow cytometry.Results. Conditioned medium from SF significantly increased monocytes viability by 60% compared to CD14+ cells cultured in medium alone(P<0.001). This effect was enhanced using conditioned media from IL-1βand TNFαstimulated SF. GM-CSF but not M-CSF nor IL34 blocking antibodies was able to significantly decrease monocyte viability by 30% when added to the conditioned media from IL-1βand TNFαstimulated SF(P<0.001). Finally, monocyte cultured in presence of SF conditioned media did not exhibit a specific M1 or M2 phenotype.Conclusion. Overall, rheumatoid arthritis synovial fibroblasts stimulated with proinflammatory cytokines (IL-1βand TNFα) promote monocyte viability via GM-CSF but do not induce a specific macrophage polarization.

وصف الملف: text/xhtml

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::156eefe1b0a7d277da15a6d539740262Test
https://doi.org/10.1155/2014/241840Test