Identification of Key Pathways and Genes in the Orai2 Mediated Classical and Mesenchymal Subtype of Glioblastoma by Bioinformatic Analyses

التفاصيل البيبلوغرافية
العنوان: Identification of Key Pathways and Genes in the Orai2 Mediated Classical and Mesenchymal Subtype of Glioblastoma by Bioinformatic Analyses
المؤلفون: Feng Yuan, Haiwen Ma, Yihan Yang, Yu Lin, Bingcheng Ren, Xuejun Yang, Long Hai, Shengping Yu, Haolang Ming, Luqing Tong, Tao Li, Li Yi, Peidong Liu, Yingshuai Wang
المصدر: Disease Markers, Vol 2019 (2019)
Disease Markers
بيانات النشر: Hindawi, 2019.
سنة النشر: 2019
مصطلحات موضوعية: 0301 basic medicine, Epithelial-Mesenchymal Transition, Databases, Factual, Article Subject, MAP Kinase Signaling System, ORAI2 Protein, Clinical Biochemistry, Apoptosis, Kaplan-Meier Estimate, Biology, Transcriptome, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Biomarkers, Tumor, Genetics, Cluster Analysis, Humans, KEGG, Molecular Biology, Gene, Survival analysis, Oligonucleotide Array Sequence Analysis, Regulation of gene expression, lcsh:R5-920, Brain Neoplasms, Biochemistry (medical), Mesenchymal stem cell, General Medicine, Prognosis, Phenotype, Gene Expression Regulation, Neoplastic, 030104 developmental biology, 030220 oncology & carcinogenesis, Cancer research, Glioblastoma, lcsh:Medicine (General), Research Article
الوصف: Background. Ca2+ release-activated Ca2+ channels (CRAC) are the main Ca2+ entry pathway regulating intracellular Ca2+ concentration in a variety of cancer types. Orai2 is the main pore-forming subunit of CRAC channels in central neurons. To explore the role of Orai2 in glioblastoma (GBM), we investigated the key pathways and genes in Orai2-mediated GBM by bioinformatic analyses. Methods. Via The Cancer Genome Atlas (TCGA), French, Sun, and Gene Expression Omnibus (GEO) (GDS3885) datasets, we collected 1231 cases with RNA-seq data and analyzed the functional annotation of Orai2 by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Univariate and multivariate survival analyses were applied to 823 patients with survival data. Results. We discovered that Orai2 was markedly upregulated in GBM compared to normal brain samples and lower-grade gliomas (LGG). Survival analysis found that higher expression of Orai2 was independently associated with a worse prognosis of patients with the classical and mesenchymal subtypes of GBM. Simultaneously, Orai2 expression was higher in tumors of the classical and mesenchymal subtypes than other subtypes and was significantly correlated with classical- and mesenchymal-related genes. GO and KEGG pathway analysis revealed that genes significantly correlated with Orai2 were involved in the JNK pathway. Through screening transcriptomic data, we found a strong association between Orai2 and apoptosis, stemness, and an epithelial-mesenchymal transition- (EMT-) like phenotype. Conclusion. As a prognostic factor, Orai2 is obviously activated in the classical and mesenchymal subtypes of GBM and promotes glioma cell self-renewal, apoptosis, and EMT-like by the JNK pathway. These findings indicate that Orai2 could be a candidate prognostic and therapeutic target, especially for the classical and mesenchymal subtypes of GBM.
وصف الملف: text/xhtml
اللغة: English
تدمد: 0278-0240
DOI: 10.1155/2019/7049294
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82843a1e843927f6fb45c41cd2615c47Test
حقوق: OPEN
رقم الانضمام: edsair.doi.dedup.....82843a1e843927f6fb45c41cd2615c47
قاعدة البيانات: OpenAIRE
الوصف
تدمد:02780240
DOI:10.1155/2019/7049294