المؤلفون: Shu Qin, Tao Liu, Dongying Zhang, Shiyong Wu, Guoqiang Cai, Yu Chen, Zhenyu Zhou

المصدر: Mediators of Inflammation
Mediators of Inflammation, Vol 2021 (2021)

مصطلحات موضوعية: Immunology, Blotting, Western, Apoptosis, Real-Time Polymerase Chain Reaction, Text mining, microRNA, Pathology, Basic Helix-Loop-Helix Transcription Factors, Human Umbilical Vein Endothelial Cells, RB1-214, Medicine, Humans, Cell Proliferation, L-Lactate Dehydrogenase, business.industry, Tumor Necrosis Factor-alpha, Endothelial Cells, Cell Biology, Atherosclerosis, Flow Cytometry, Healthy Volunteers, Endothelial stem cell, MicroRNAs, Cancer research, business, Corrigendum, Reactive Oxygen Species

الوصف: Atherosclerosis is one of the leading causes of mortality worldwide. Growing evidence suggested that miRNAs contributed to the progression of atherosclerosis. miR-30-5p was found involved in various diseases. However, the role of miR-30-5p in regulation of atherosclerosis is not known. Here, we aim to investigate the effects of miR-30-5p on regulating the progression of atherosclerosis. The expression levels of miR-30-5p in serum collected from atherosclerosis patients and normal healthy people were analyzed by qRT-PCR. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway bioinformatics were carried out to reveal the possible signaling pathways involved in the mode of action of miR-30-5p. A potential target gene of miRNA-30-5p was searched and examined by a luciferase reporter assay. ELISA, Western blot, proliferation, and flow cytometry assays were performed to assess the biological functional role of miR-30-5p

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82d71dd2b8004ab882b3bb79a121d9c1Test
http://europepmc.org/articles/PMC8556131Test

المؤلفون: Xiangsheng Xiao, Jun Tang, Xi Wang, Weidong Wei, Liqin Ping, Gehao Liang, Yan Wang, Xiaoming Xie, Tian Du, Kaiming Zhang, Ya Sun

المصدر: Oxidative Medicine and Cellular Longevity
Oxidative Medicine and Cellular Longevity, Vol 2021 (2021)

مصطلحات موضوعية: Oncology, Aging, medicine.medical_specialty, Article Subject, Breast Neoplasms, medicine.disease_cause, Biochemistry, chemistry.chemical_compound, Breast cancer, Internal medicine, Lactate dehydrogenase, medicine, Humans, Blood urea nitrogen, Creatinine, QH573-671, business.industry, Area under the curve, Cell Biology, General Medicine, Nomogram, Middle Aged, medicine.disease, Prognosis, Survival Analysis, Oxidative Stress, chemistry, Female, Cytology, business, TBIL, Oxidative stress, Research Article

الوصف: Background. Breast cancer was associated with imbalance between oxidation and antioxidation. Local oxidative stress in tumors is closely related to the occurrence and development of breast cancer. However, the relationship between systematic oxidative stress and breast cancer remains unclear. This study is aimed at exploring the prognostic value of systematic oxidative stress in patients with operable breast cancer. Methods. A total of 1583 operable female breast cancer patients were randomly assigned into the training set and validation set. The relationship between systematic oxidative stress biomarkers and prognosis were analyzed in the training and validation sets. Results. The systematic oxidative stress score (SOS) was established based on five systematic oxidative stress biomarkers including serum creatinine (CRE), serum albumin (ALB), total bilirubin (TBIL), lactate dehydrogenase (LDH), and blood urea nitrogen (BUN). SOS was an independent prognostic factor for operable breast cancer patients. A nomogram based on SOS and clinical characteristics could accurately predict the prognosis of operable breast cancer patients, and the area under the curve (AUC) of the nomogram was 0.823 in the training set and 0.872 in the validation set, which was much higher than the traditional prognostic indicators. Conclusions. SOS is an independent prognostic indicator for operable breast cancer patients. A prediction model based on SOS could accurately predict the outcome of operable breast cancer patients.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95f6c98d5ab1110ed6950871b32a52e0Test
http://europepmc.org/articles/PMC8516560Test

المؤلفون: Changxing Hu, Chao Chang, Qingwei Ji, Zicong Yang, Jianwei Zhang, Zhishan Liang, Ling Liu, Mengjie Wang, Lei Shi

المصدر: Mediators of Inflammation
Mediators of Inflammation, Vol 2021 (2021)

مصطلحات موضوعية: Cardiac function curve, Male, Article Subject, Immunology, Inflammation, Apoptosis, Pharmacology, chemistry.chemical_compound, Mice, Troponin complex, Lactate dehydrogenase, Pathology, polycyclic compounds, Medicine, RB1-214, Animals, Doxorubicin, Myocytes, Cardiac, Interleukin-16, biology, business.industry, Macrophages, Cell Differentiation, Cell Biology, Antibodies, Neutralizing, Cardiotoxicity, Mice, Inbred C57BL, Myocarditis, chemistry, biology.protein, Creatine kinase, Interleukin 16, medicine.symptom, business, medicine.drug, Research Article

الوصف: Background. Interleukin-16 (IL-16) is an important inflammatory regulator and has been shown to have a powerful effect on the regulation of the inflammatory response. Cardiac inflammation has been reported to be closely related to doxorubicin- (DOX-) induced cardiac injury. In this study, the role of IL-16 in DOX-induced cardiac injury and the possible mechanisms were examined. Methods. Cardiac IL-16 levels were first measured in DOX- or saline-treated mice. Additionally, mice were pretreated with the anti-IL-16-neutralizing antibody (nAb) or isotype IgG for 1 day and further administered DOX or saline for 5 days. Then, cardiac injury, cardiac M1 macrophage levels, and cardiomyocyte apoptosis were analyzed. The effects of the anti-IL-16 nAb on macrophage differentiation and cardiomyocyte apoptosis were also investigated in vitro. Results. DOX administration increased IL-16 expression in cardiac macrophages compared with that of saline treatment. The anti-IL-16 nAb significantly decreased serum levels of lactate dehydrogenase (LDH), myocardial-bound creatine kinase (CK-MB), and cardiac troponin T (cTnT) and elevated cardiac function in DOX-induced mice. Treatment with the anti-IL-16 nAb also reduced p65 pathway activation, decreased M1 macrophage-related marker and cytokine expression, and protected against cardiomyocyte apoptosis in DOX-induced mice. In cell studies, the anti-IL-16 nAb also reduced DOX-induced M1 macrophage differentiation and alleviated apoptosis in cardiomyocytes cocultured with macrophages. Conclusions. The anti-IL-16 nAb protects against DOX-induced cardiac injury by reducing cardiac inflammation, and IL-16 may be a promising target to prevent DOX-related cardiac injury.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0496ad3f402ad46ff91b63835d7b373Test
http://europepmc.org/articles/PMC8075660Test

المؤلفون: Zhe Cheng, Xiyang Yang, Minghao Luo, Dingyi Lv, Yongzheng Guo, Bi Huang, Ruiyu Wang, Longxiang Huang, Jian Shen, Xiang Li, Jianghong Yan, Suxin Luo

المصدر: Oxidative Medicine and Cellular Longevity
Oxidative Medicine and Cellular Longevity, Vol 2021 (2021)

مصطلحات موضوعية: Cardiac function curve, Male, Aging, SIRT3, Article Subject, Ischemia, Apoptosis, Myocardial Reperfusion Injury, Pharmacology, medicine.disease_cause, Protective Agents, urologic and male genital diseases, Biochemistry, chemistry.chemical_compound, Mice, Lactate dehydrogenase, Sirtuin 3, medicine, Animals, Myocardial infarction, QH573-671, business.industry, Cell Biology, General Medicine, Saponins, medicine.disease, Triterpenes, Mice, Inbred C57BL, Oxidative Stress, chemistry, Gene Expression Regulation, Cytology, business, Reperfusion injury, Oxidative stress, Research Article

الوصف: Myocardial ischemia-reperfusion injury (MIRI) is a phenomenon that reperfusion leads to irreversible damage to the myocardium and increases mortality in acute myocardial infarction (AMI) patients. There is no effective drug to treat MIRI. Tubeimoside I (TBM) is a triterpenoid saponin purified from Chinese traditional medicine tubeimu. In this study, 4 mg/kg TBM was given to mice intraperitoneally at 15 min after ischemia. And TBM treatment improved postischemic cardiac function, decreased infarct size, diminished lactate dehydrogenase release, ameliorated oxidative stress, and reduced apoptotic index. Notably, ischemia-reperfusion induced a significant decrease in cardiac SIRT3 expression and activity, while TBM treatment upregulated SIRT3’s expression and activity. However, the cardioprotective effects of TBM were largely abolished by a SIRT3 inhibitor 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP). This suggests that SIRT3 plays an essential role in TBM’s cardioprotective effects. In vitro, TBM also protected H9c2 cells against simulated ischemia/reperfusion (SIR) injury by attenuating oxidative stress and apoptosis, and siSIRT3 diminished its protective effects. Taken together, our results demonstrate for the first time that TBM protects against MIRI through SIRT3-dependent regulation of oxidative stress and apoptosis. TBM might be a potential drug candidate for MIRI treatment.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::446e5cd2772d16431aec304fe5b84322Test

المؤلفون: Yu Zhang, Ziming Zheng, Qilin Zhang

المصدر: Disease Markers, Vol 2021 (2021)
Disease Markers

مصطلحات موضوعية: Adult, Male, Medicine (General), 2019-20 coronavirus outbreak, medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), Article Subject, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), education, Clinical Biochemistry, Inflammation, Logistic regression, behavioral disciplines and activities, Enzyme abnormality, R5-920, Internal medicine, Genetics, medicine, Creatine Kinase, MB Form, Humans, Molecular Biology, Retrospective Studies, L-Lactate Dehydrogenase, business.industry, Myocardium, Troponin I, Biochemistry (medical), COVID-19, Alanine Transaminase, Retrospective cohort study, General Medicine, Middle Aged, Enzymes, Normal group, Logistic Models, Female, medicine.symptom, business, psychological phenomena and processes, Research Article

الوصف: Background. It has been observed that COVID-19 may cause myocardial damage, but there are few detailed reports on myocardial enzyme abnormalities. Methods. In this retrospective study, we analyzed data from 157 consecutive laboratory-confirmed and hospitalized COVID-19 patients from Wuhan. We collected information on demographic and clinical characteristics, laboratory findings, and clinical outcomes. Logistic regression analysis was used to explore the risk factors associated with the severity of COVID-19. The association between myocardial enzyme abnormalities and the mortality was also investigated. Results. The mortality in abnormal myocardial enzyme group was obviously higher than the normal group ( P < 0.001 ). The majority of patients ( n = 72 , 97.3%) with normal cardiac enzyme group were of the common novel coronavirus pneumonia (NCP) type, whereas half of the patients with cardiac enzyme abnormalities ( n = 40 , 48.2%) developed critical and severe NCP type. The multivariable logistic regression analysis indicated that COVID-19 patients with increasing age ( P = 0.035 ), higher levels of CRP ( P = 0.038 ), and TNI ( P = 0.036 ) were associated with increased death than other patients. Conclusions. Myocardial enzyme abnormality and myocardial injury were associated with the severity and fatal outcomes of COVID-19. Clinicians should pay attention to the markers of myocardial injury in COVID-19 patients, especially those with older age, comorbidities, and inflammation.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4912d65cec5d6745e91befd4a865b7d2Test

المؤلفون: Sridhar Chilimuri, Arlene Tieng, Giovanni Franchin, Tegveer Sandhu

المصدر: The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien des Maladies Infectieuses et de la Microbiologie Médicale
Canadian Journal of Infectious Diseases and Medical Microbiology, Vol 2020 (2020)

مصطلحات موضوعية: Microbiology (medical), Moderate to severe, medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), Article Subject, Infectious and parasitic diseases, RC109-216, 030204 cardiovascular system & hematology, Gastroenterology, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Acute pericarditis, Lactate dehydrogenase, Internal medicine, medicine, Colchicine, 030203 arthritis & rheumatology, biology, business.industry, Case-control study, medicine.disease, QR1-502, Ferritin, Clinical trial, Infectious Diseases, chemistry, biology.protein, business, Research Article

الوصف: Background. Colchicine has been used in conditions such as periodic febrile illness, acute pericarditis, and gouty arthritis, all having a common hyperinflammatory response as seen in moderate to severe forms of coronavirus disease 2019 (COVID-19). This project was carried out during the rapid surge of cases in New York City, and the goal was to assess the efficacy of colchicine in treating patients with COVID-19. Methods. Patients admitted to two distinct pulmonary oriented floors of the BronxCare Hospital Center were compared. Patients on one floor were given colchicine in addition to standard of care, while control patients from another floor received only standard of care. Patients who had at least two separate timepoint measurements for at least two out of four serum inflammatory markers (C-reactive protein (CRP), D-dimer, ferritin, or lactate dehydrogenase (LDH)) were selected for the final comprehensive analysis. Results. An initial analysis performed on all patients, irrespective of the availability of two timepoint inflammatory markers, revealed a lower mortality (49.1% versus 72.9%, P = 0.002 ), a lower percentage of intubations (52.8% versus 73.6%, P = 0.006 ), and a higher discharge rate (50.9% versus 27.1%, P = 0.002 ), in the patients who received colchicine. Patients in the final comprehensive analysis groups (34 in the colchicine group and 78 in the control group) had a similar prevalence of comorbid medical conditions, except for renal failure, which was higher in the control group (65.3% versus 35.2%, P = 0.015 ). HTN (71.8% versus 52.9%, P = 0.053 ) and DM (51.3% versus 32.4%, P = 0.064 ) were also more prevalent in the control group, although the difference was not statistically significant. Patients who received colchicine had a lower mortality than the control group (47.1% versus 80.8%, P = 0.0003 ), lower rate of intubations (47.1% versus 87.2%, P < 0.0001 ), and a higher discharge rate (52.9% versus 19.2%, P = 0.0003 ). Patients in the colchicine group also showed a more significant decrease in inflammatory markers for D-dimer ( P = 0.037 ), CRP ( P = 0.014 ), and ferritin ( P = 0.012 ). Conclusions. Our study demonstrates that colchicine improved outcomes in patients with COVID-19 receiving standard of care therapy. Future randomized, placebo-controlled clinical trials to assess the potential benefit of colchicine in COVID-19 are warranted.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9300674f64c3fc7408741fa00b6b2491Test

المؤلفون: Meng Wang, Yanqiu Lai, Jielin Deng, Yifeng Zhang, Xiaoya Zhou, Hong Jiang, Qianqian Zhang, Xiaomeng Yang, Menglong Wang, Zhenya Wang

المصدر: Oxidative Medicine and Cellular Longevity
Oxidative Medicine and Cellular Longevity, Vol 2019 (2019)

مصطلحات موضوعية: Male, Aging, medicine.medical_specialty, Article Subject, Vagus Nerve Stimulation, NF-E2-Related Factor 2, medicine.medical_treatment, Ischemia, Inflammation, Apoptosis, medicine.disease_cause, Biochemistry, Rats, Sprague-Dawley, chemistry.chemical_compound, Internal medicine, Lactate dehydrogenase, medicine, Animals, lcsh:QH573-671, business.industry, lcsh:Cytology, Liver Diseases, Sham surgery, Cell Biology, General Medicine, medicine.disease, Vagus nerve, Rats, Disease Models, Animal, Oxidative Stress, Endocrinology, chemistry, Reperfusion Injury, Heme Oxygenase (Decyclizing), medicine.symptom, business, Reperfusion injury, Vagus nerve stimulation, Oxidative stress, Research Article

الوصف: It has been demonstrated that vagus nerve stimulation (VNS) plays a protective role in ischemia/reperfusion (I/R) injury of various organs. The present study investigates the protective effect of VNS on hepatic I/R injury and the potential mechanisms. Male Sprague-Dawley rats were randomly allocated into three groups: the sham operation group (Sham; n=6, sham surgery with sham VNS); the I/R group (n=6, hepatic I/R surgery with sham VNS); and the VNS group (n=6, hepatic I/R surgery plus VNS). The I/R model was established by 1 hour of 70% hepatic ischemia. Tissue samples and blood samples were collected after 6 hours of reperfusion. The left cervical vagus nerve was separated and stimulated throughout the whole I/R process. The stimulus intensity was standardized to the voltage level that slowed the sinus rate by 10%. VNS significantly reduced the necrotic area and cell death in I/R tissues. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) were also decreased by VNS. In addition, VNS suppressed inflammation, oxidative stress, and apoptosis in I/R tissues. VNS significantly increased the protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) in the liver. These data indicated that VNS may attenuate hepatic I/R injury by inhibiting inflammation, oxidative stress, and apoptosis possibly via the Nrf2/HO-1 pathway.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f69752c760ec806e4b2f790926b5b712Test
http://europepmc.org/articles/PMC6530204Test

المؤلفون: Huojin Deng, Tao Zhu, Laifu Li, Chanmei Shi, Jingqi Min, Kunlun Huang, Hua-peng Yu

المصدر: Mediators of Inflammation, Vol 2019 (2019)
Mediators of Inflammation

مصطلحات موضوعية: Male, Curcumin, Article Subject, Immunology, Lung injury, Pharmacology, medicine.disease_cause, Epithelial Damage, chemistry.chemical_compound, Mice, Western blot, Lactate dehydrogenase, Malondialdehyde, medicine, lcsh:Pathology, Animals, Lung, Glutathione Peroxidase, medicine.diagnostic_test, business.industry, Superoxide Dismutase, Tumor Necrosis Factor-alpha, Cell Biology, Lung Injury, Oxidative Stress, medicine.anatomical_structure, chemistry, Alkaline phosphatase, Particulate Matter, business, Bronchoalveolar Lavage Fluid, Oxidative stress, Research Article, lcsh:RB1-214

الوصف: Background. Epidemiological studies have shown that exposure to PM induces oxidative stress, leading to a variety of health problems. In particular, PM2.5 contains a lot of substances harmful to the human body and penetrates into the lungs to induce lung injury. At the same time, there is increasing evidence that oxidative stress also affects the severity of lung injury. However, there is still no good way to reduce or eliminate these hazards. In the future, more experimental research is needed to further confirm the mechanisms of these hazards and formulate effective preventive measures and treatment plans for their hazard mechanisms. Curcumin has been reported to reduce oxidative stress and inflammatory damage and protect organs. Objective. To investigate whether curcumin can play a protective role against PM2.5-induced oxidative stress and inflammatory damage by inducing expression of the HO-1/CO/P38 MAPK pathway. Methods. In this experiment, PM2.5 was dropped into the trachea to establish a lung injury model in mice. 28 SPF-grade male Kunming mice were randomly divided into 4 groups: normal control group, saline control group, PM2.5 treatment group, and curcumin intervention group. Albumin (ALB), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) were measured in alveolar lavage fluid (BALF) to assess lung tissue damage. Colorimetric detection of oxidative stress indicators such as MDA, GSH-PX, T-AOC, and CAT in the lung tissue was performed. The levels of IL-6 and TNF-α in the lung tissue were determined by ELISA. Histopathological examination was used for the assessment of alveolar epithelial damage. The protein expression of the HO-1/P38 MAPK pathway in the lung tissue was determined by Western blot and immunohistochemistry. Endogenous CO was detected by spectrophotometry. The results showed that the expression of the HO-1/CO/P38 MAPK protein in the lung tissue was significantly increased in the curcumin intervention group compared with the PM2.5 treatment group, and it was statistically significant (P<0.05). Compared with the PM2.5 treatment group, the curcumin intervention group can reduce the amount of ALB, LDH, and ALP in BALF; reduce the levels of MDA, IL-1, and TNF-α in the lung tissue; and improve GSH-PX, T-AOC, and CAT levels, but there is no statistical difference (P>0.05). Conclusion. We found that PM2.5 can cause lung damage through oxidative stress and inflammatory responses. Oxidative stress and inflammatory responses increase the expression of HO-1/CO/P38 MAPK. The intervention of curcumin can further increase the expression of HO-1/CO/P38 MAPK.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::704b05053f247e373952739d73fcc691Test

المؤلفون: Hendrik Isbarn, Henrik Zecha, Uwe Pichlmeier, Petra Anheuser, Klaus-Peter Dieckmann, Magdalena Kulejewski, Hanna Simonsen-Richter

المصدر: BioMed Research International, Vol 2019 (2019)
BioMed Research International

مصطلحات موضوعية: Male, 0301 basic medicine, medicine.medical_specialty, Younger age, Article Subject, lcsh:Medicine, Chorionic Gonadotropin, Gastroenterology, General Biochemistry, Genetics and Molecular Biology, Cohort Studies, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Testicular Neoplasms, Internal medicine, Lactate dehydrogenase, Biomarkers, Tumor, medicine, Humans, Neoplasm Metastasis, Stage (cooking), Beta human chorionic gonadotropin, Neoplasm Staging, General Immunology and Microbiology, business.industry, lcsh:R, Age Factors, Histology, General Medicine, Neoplasms, Germ Cell and Embryonal, Response to treatment, Testicular germ cell, Seminoma, Tumor Burden, Treatment Outcome, 030104 developmental biology, chemistry, 030220 oncology & carcinogenesis, alpha-Fetoproteins, Neoplasm Recurrence, Local, business, Research Article, Cohort study

الوصف: Introduction. Although serum tumor markers beta human chorionic gonadotropin (bHCG), alpha-fetoprotein (AFP), and lactate dehydrogenase (LDH) are well-established tools for the management of testicular germ cell tumours (GCTs), there are only few data from contemporary cohorts of primary GCT patients regarding these biomarkers. Our aim was to evaluate marker elevations in testicular GCTs and to document their associations with various clinical characteristics.Patients and Methods. A total of 422 consecutive patients with GCTs were retrospectively analysed regarding serum levels of bHCG, AFP, and LDH during the course of treatment. Additionally, the following characteristics were recorded: histology, age, laterality, clinical stage (CS), pT-stage, and tumour size. Marker elevations were first tabulated in dichotomized way (elevated: yes/no) in various subgroups and second as continuous measured serum values. Descriptive statistical methods were employed to look for differences among subgroups and for associations of elevations with clinical parameters.Results. In all GCT patients, the frequencies of elevated levels of bHCG, AFP, LDH, and bHCG or AFP were 37.9%, 25.6%, 32.9%, and 47.6%; in pure seminomas 28%, 2.8%, 29.1%, and 30.3%; and in nonseminoma 53.0%, 60.1%, 38.7%, and 73.8%. Significant associations were noted with pT-stages >pT1, clinical stages >CS1, tumour size, and younger age. Frequencies of marker elevations dropped significantly after treatment, but LDH levels remained elevated in 30.5%-34.1%. Relapsing patients (n=27) had elevated levels of bHCG, AFP, and LDH in 25.9%, 22.2%, and 29.6%, respectively, thirteen of whom with a changed marker pattern.Conclusions. The classical GCT-biomarkers correlate with treatment success. Clinical utility is limited due to proportions of < 50% of patients with elevated levels and the low specificity of LDH. The elevation rates are significantly associated with histology, clinical and pT-stages, tumour size, and younger age. Individual marker patterns may change upon relapse. Clinically, ideal biomarkers are yet to be found.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0664e676fe8d139c8b194cde74a63ea6Test

المؤلفون: Xiu-mei Ren, Qing-Sheng You, Wei-Nan Li, Qing Ye, Ming-Ming Gu, Shuo Wang, Kun Liu, Fei Wang, Chun-Hui Ma

المصدر: BioMed Research International
BioMed Research International, Vol 2018 (2018)

مصطلحات موضوعية: Blood Platelets, 0301 basic medicine, Heart Injury, Article Subject, Cyclophosphamide, lcsh:Medicine, Pharmacology, General Biochemistry, Genetics and Molecular Biology, Mice, 03 medical and health sciences, White blood cell, Leukocytes, medicine, Animals, Humans, Platelet, Aspartate Aminotransferases, Creatine Kinase, Pathological, L-Lactate Dehydrogenase, General Immunology and Microbiology, biology, business.industry, lcsh:R, NF-kappa B, Alanine Transaminase, Heart, General Medicine, 030104 developmental biology, medicine.anatomical_structure, Heart Injuries, biology.protein, Creatine kinase, Dangguibuxue decoction, Signal transduction, business, Drugs, Chinese Herbal, Signal Transduction, medicine.drug, Research Article

الوصف: Dangguibuxue decoction (DBD), a kind of Chinese herbal medicine, has been widely used to treat blood deficiency disease in China. In this experiment, we studied the effects of the Dangguibuxue decoction (DBD) on the myocardial injury induced by cyclophosphamide in mice. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase (CK), and lactic dehydrogenase (LDH) in serum were detected by commercial kits. Total white blood cell (WBCs), platelets, and cytokines pathological changes of heart tissue were also examined. In addition, the protein levels of the NF-кB pathway were detected to reveal its mechanism. The results showed that DBD significantly decreased the levels of ALT, AST, CK, and LDH and increased WBCs in CTX-induced mice. In addition, DBD significantly alleviated pathological changes of heart tissue. DBD significantly reduced the protein expressions of NF-кB signaling pathway. In summary, DBD can be considered an effective drug to alleviate CTX-induced heart damage in mice.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb33bd15728458429ac3adb78d08bbacTest
http://europepmc.org/articles/PMC6236918Test