رسالة جامعية
Inflammation and Degeneration in the Saccular Intracranial Aneurysm Wall : The Role of Serum Amyloid A, Lymphatic Neovessels, and T-Lymphocytes
| العنوان: | Inflammation and Degeneration in the Saccular Intracranial Aneurysm Wall : The Role of Serum Amyloid A, Lymphatic Neovessels, and T-Lymphocytes |
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| المؤلفون: | Huuska, Nora |
| المساهمون: | Eide, Per Kristian, Tulamo, Riikka, Netti, Eliisa, Helsingin yliopisto, lääketieteellinen tiedekunta, Biolääketieteellinen tohtoriohjelma, Helsingfors universitet, medicinska fakulteten, Doktorandprogrammet i biomedicin, University of Helsinki, Faculty of Medicine, Doctoral Programme in Biomedicine, Neurosurgery Research Group, Biomedicum Helsinki, Helsinki University Hospital and University of Helsinki |
| بيانات النشر: | Helsingin yliopisto Helsingfors universitet University of Helsinki |
| سنة النشر: | 2024 |
| المجموعة: | Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
| مصطلحات موضوعية: | neurokirurgia |
| الوصف: | Background and Aim Aneurysmal subarachnoid hemorrhages (SAH), resulting from the rupture of an intracranial aneurysm, pose a significant health risk with notable mortality and morbidity rates. Saccular intracranial aneurysms (sIA), characterized by a localized pouch-like protrusion from cerebral arteries, are estimated to impact 2–3% of the global population. Current treatment options are invasive and carry risks, lacking a pharmacological alternative. Importantly, not all sIAs rupture during a patient’s lifetime, emphasizing the need to understand the pathophysiological mechanisms governing their formation and rupture. This thesis focuses on the chronic inflammation of sIA walls, investigating the roles of various immune cell types, including CD4+ and CD8+ T-lymphocytes, and CD20+ B-lymphocytes, along with the inflammatory mediator serum amyloid A (SAA). Furthermore, the study examines the presence of lymphatic neovessels within sIA walls. Research dedicated to unraveling the pathophysiological mechanisms leading to the formation and rupture of sIAs may provide insights into potential therapeutic interventions for this debilitating disease. Materials and Methods A total of 36 sIA samples, comprising 16 unruptured and 20 ruptured cases, were obtained during surgical clip ligation at Helsinki University Hospital. Histological, immunohistochemical (IHC), and immunofluorescence (IF) stainings were conducted on the sIA samples. IHC stainings targeted SAA and lymphatic endothelial cell (LEC) markers prospero-related homeobox 1 (Prox1), lymphatic vessel endothelial hyaluronic acid receptor-1 (LYVE-1), podoplanin, and vascular endothelial growth factor receptor 3 (VEGFR-3), while multiplexed IF staining detected different immune cell types, including CD3+, CD4+, and CD8+ T-lymphocytes, in the sIA walls. The subsequent analysis involved semiquantitative evaluation of SAA and LEC markers, assessing the presence and number of lymphatic vessels, and determining inflammatory cell densities. The results were compared with ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| وصف الملف: | application/pdf |
| اللغة: | English |
| ردمك: | 978-951-51-9812-9 978-951-51-9811-2 951-51-9812-7 951-51-9811-9 |
| تدمد: | 2954-2952 |
| العلاقة: | URN:ISBN:978-951-51-9812-9; Joensuu: 2024, Dissertationes Universitatis Helsingiensis. 2954-2898; Dissertationes Universitatis Helsingiensis; URN:ISSN:2954-2952; URN:ISBN:978-951-51-9811-2; http://hdl.handle.net/10138/575148Test |
| الإتاحة: | http://hdl.handle.net/10138/575148Test |
| حقوق: | Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty. ; This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited. ; Publikationen är skyddad av upphovsrätten. Den får läsas och skrivas ut för personligt bruk. Användning i kommersiellt syfte är förbjuden. |
| رقم الانضمام: | edsbas.D4AE373D |
| قاعدة البيانات: | BASE |
| ردمك: | 9789515198129 9789515198112 9515198127 9515198119 |
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| تدمد: | 29542952 |