المؤلفون: Bouzid, Assia, Arous, Ali, Cheikh Felouah, Oum, Merah, Othmane, Adda, Ahmed

المساهمون: Université Ibn Khaldoun (UIK), Université de Djilali Bounaama Khemis Miliana (univ-DBKM), Chimie Agro-Industrielle (CAI), Ecole nationale supérieure des ingénieurs en arts chimiques et technologiques (ENSIACET), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

المصدر: ISSN: 1211-8516.

مصطلحات موضوعية: durum wheat, drought, grain weight, starch, amylose/amylopectin ratio, photosynthetic activity, remobilization, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences

الوصف: International audience ; In Algeria, drought affects grain weight and modified its biochemical composition. The present study was conducted to evaluate the effect of two water supplies (100% FC, 30% FC) on grain weight and composition of five genotypes of durum wheat (Triticum durum Desf.). We also examined the effects of shading of peduncle, spike and the entire plant and excision of awns, flag leaves and foliar system on grain weight, starch and amylose/amylopectin ratio. From this study, we found that grain weight was significantly reduced by the application of water deficit. However, this action is greatly related to genotype type. It is admitted that this trait is strongly conditioned by the grain filling process. The morphological characteristics of plants were implicated to grain weight elaboration. Among organs studied, the photosynthetic rate of spick and reserves remobilization from stem announced determinant in grain filling. We found also that starch content which is associated to amylose/amylopectin ratio is strongly related to grain weigh. Finally, grains weight variations were associated to starch content and mainly related to the water supply condition.

العلاقة: hal-03100847; https://hal.inrae.fr/hal-03100847Test; https://hal.inrae.fr/hal-03100847/documentTest; https://hal.inrae.fr/hal-03100847/file/actaun_2020068060937.pdfTest

الإتاحة: https://doi.org/10.11118/actaun202068060937Test
https://hal.inrae.fr/hal-03100847Test
https://hal.inrae.fr/hal-03100847/documentTest
https://hal.inrae.fr/hal-03100847/file/actaun_2020068060937.pdfTest

المؤلفون: Mari, Stéphane, Bailly, Christophe, Thomine, Sébastien

المساهمون: Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de Biologie du Développement de Villefranche sur mer (LBDV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), ANR-16-CE20-0019,ISISTOR,Amélioration du contenu en fer de la graine(2016)

المصدر: ISSN: 0264-6021.

مصطلحات موضوعية: iron, biofortification, nutrition, remobilization, seed, vacuole, [SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology

الوصف: International audience ; To ensure the success of the new generation in annual species, the mother plant transfers a large proportion of the nutrients it has accumulated during its vegetative life to the next generation through its seeds. Iron (Fe) is required in large amounts to provide the energy and redox power to sustain seedling growth. However, free Fe is highly toxic as it leads to the generation of reactive oxygen species. Fe must, therefore, be tightly bound to chelating molecules to allow seed survival for long periods of time without oxidative damage. Nevertheless, when conditions are favorable, the seed's Fe stores have to be readily remobilized to achieve the transition toward active photosynthesis before the seedling becomes able to take up Fe from the environment. This is likely critical for the vigor of the young plant. Seeds constitute an important dietary source of Fe, which is essential for human health. Understanding the mechanisms of Fe storage in seeds is a key to improve their Fe content and availability in order to fight Fe deficiency. Seed longevity, germination efficiency and seedling vigor are also important traits that may be affected by the chemical form under which Fe is stored. In this review, we summarize the current knowledge on seed Fe loading during development, long-term storage and remobilization upon germination. We highlight how this knowledge may help seed Fe biofortification and discuss how Fe storage may affect the seed quality and germination efficiency.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/31950999; hal-02445553; https://hal.science/hal-02445553Test; https://hal.science/hal-02445553/documentTest; https://hal.science/hal-02445553/file/Mari%20et%20al%20Biochemical%20Journal%202020.pdfTest; PRODINRA: 492706; PUBMED: 31950999; WOS: 000512948100016

الإتاحة: https://doi.org/10.1042/BCJ20190188Test
https://hal.science/hal-02445553Test
https://hal.science/hal-02445553/documentTest
https://hal.science/hal-02445553/file/Mari%20et%20al%20Biochemical%20Journal%202020.pdfTest

المؤلفون: Mazlouzi, Mohamed, Morel, Christian, Chesseron, Coralie, Robert, Thierry, Mollier, Alain

المساهمون: Interactions Sol Plante Atmosphère (UMR ISPA), Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

المصدر: ISSN: 1664-462X ; Frontiers in Plant Science ; https://hal.inrae.fr/hal-02907180Test ; Frontiers in Plant Science, Frontiers, 2020, 11, pp.1-10. ⟨10.3389/fpls.2020.00870⟩.

مصطلحات موضوعية: P remobilization, Post-anthesis P uptake, Grain P concentrations, 32 P tracer, Phosphorus, Durum wheat, Hydroponic conditions, [SDE]Environmental Sciences, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Phosphorus (P) in durum wheat grains after anthesis originates from either the external P source or the internal remobilization of P from different plant organs. The supply of P and its use by the plant are important factors that can affect the contribution of each source to grain P nutrition. Thus, this experiment aimed to quantify the origin of P in grains of durum wheat plants with different P nutritional status. Wheat plants were grown from juvenile stages to maturity in complete nutrient solutions with either high (0.125 mM) or low (0.025 mM) P concentrations in greenhouse conditions. Phosphorus in nutrient solutions was spiked by introducing 32 P after anthesis to quantify the external P uptake and its partitioning within plant organs (spikelets, leaves, stems, roots, and post-anthesis tillers) and grains. Phosphorus use efficiency in durum wheat plants was also determined. The low and high P supply resulted in two highly different plant nutritional P status. Plants with low P status remobilized most of their stored P in all organs and allocated more than 72% of post-anthesis P uptake to grain P nutrition, whereas in the high P plants this was only 56%. Enhanced remobilization of P and the efficient allocation of newly acquired P to grains were crucial for durum wheat grain P nutrition grown under low P supply. The remobilization of P represented 81% of grain P in low P plants while it represented 65% for high P plants. Organs that contributed the most to P remobilization in low P plants were spikelets (43%) and leaves (35%). The post-anthesis tiller development was reduced in low P plants suggesting a preferential allocation of P to grains under this treatment. We concluded that P loading into grains in durum wheat is mainly derived from the remobilization of internal P sources stored before anthesis, even at high external P supply during grain filling.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/32625228; hal-02907180; https://hal.inrae.fr/hal-02907180Test; https://hal.inrae.fr/hal-02907180/documentTest; https://hal.inrae.fr/hal-02907180/file/2020_Mollier_FPLS.pdfTest; WOS: 000546826300001; PUBMED: 32625228

الإتاحة: https://doi.org/10.3389/fpls.2020.00870Test
https://hal.inrae.fr/hal-02907180Test
https://hal.inrae.fr/hal-02907180/documentTest
https://hal.inrae.fr/hal-02907180/file/2020_Mollier_FPLS.pdfTest

المؤلفون: Yan, Bo-Fang, Nguyen, Christophe, Cornu, Jean-Yves, Schönholzer-Mauclaire, Laurie, Neff, Christoph, Günther, Detlef, Frossard, Emmanuel

المساهمون: Sun Yat-sen University Guangzhou (SYSU), Department of Environmental Systems Science ETH Zürich (D-USYS), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology Zürich (ETH Zürich), Interactions Sol Plante Atmosphère (UMR ISPA), Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Department of Chemistry and Applied Biosciences ETH Zürich (D-CHAB)

المصدر: ISSN: 0032-079X.

مصطلحات موضوعية: Trace metal, Mineral nutrition, Cereal, Source tracing, Remobilization, [SDE]Environmental Sciences

الوصف: International audience ; Background and aimsCereals can be made safer and more nutritious by reducing cadmium (Cd) and enhancing zinc (Zn) levels. To respectively regulate the accumulation of these chemically similar elements in grains, it is essential to understand the differences between Cd and Zn allocation to grains.MethodsIn durum wheat (Triticum durum), dual-isotope (Cd-111 and Zn-67) labeling was used to trace the post-anthesis uptake fluxes separately from the remobilization of pre-anthesis vegetative pools. Laser ablation inductively coupled mass spectrometry was used to investigate the spatial distribution of Cd and Zn in the uppermost node.ResultsAmong the shoot organs, both pre- and post-anthesis derived Cd was more allocated to the high-transpiring organs (i.e., bracts and flag leaves) whereas Zn was more to the grain. Cadmium was likely less efficiently transferred from the xylem to the phloem as suggested by the elemental maps which showed that Cd was more abundant than Zn in the xylem of the uppermost node. Furthermore, unlike Zn, Cd was not significantly remobilized from the high-transpiring organs, which further limited the allocation of Cd to the grain.ConclusionHigh-transpiring organs are sources of grain Zn but irreversible sinks of Cd. Agronomic strategies that enhance Cd sequestration and Zn remobilization in high-transpiring organs could contribute to producing grains with low Cd and high Zn concentrations.

العلاقة: hal-04095984; https://hal.inrae.fr/hal-04095984Test; WOS: 000961829700002

الإتاحة: https://doi.org/10.1007/s11104-023-06005-7Test
https://hal.inrae.fr/hal-04095984Test

المؤلفون: Gallardo, Karine, Besson, Alicia, Klein, Anthony, Le Signor, Christine, Aubert, Gregoire, Henriet, Charlotte, Terezol, Morgane, Pateyron, Stephanie, Sanchez, Myriam, Trouverie, Jacques, Avice, Jean-Christophe, Larmure, Annabelle, Salon, Christophe, Balzergue, Sandrine, Burstin, Judith

المساهمون: Agroécologie Dijon, Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté COMUE (UBFC), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), French National Research Agency (ANR) ANR-09-GENM-026, ANR-09-GENM-0026,GENOPEA,Génomique comparative du cycle de l'azote entre M. truncatula et le pois : étude des potentialités de transfert des connaissances entre espèces modèles et cultivées(2009)

المصدر: ISSN: 1664-462X ; Frontiers in Plant Science ; https://institut-agro-dijon.hal.science/hal-02270632Test ; Frontiers in Plant Science, 2019, 10 (1014), ⟨10.3389/fpls.2019.01014⟩.

مصطلحات موضوعية: Legumes, Leaves, Reproductive period, Nitrogen remobilization, Transcriptomics, Co-expression, Transcription factors, Transporters, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology

الوصف: International audience ; Pea (Pisum sativum L.) is an important source of dietary proteins. Nutrient recycling from leaves contributes to the accumulation of seed proteins and is a pivotal determinant of protein yields in this grain legume. The aim of this study was to unveil the transcriptional regulations occurring in pea leaves before the sharp decrease in chlorophyll breakdown. As a prelude to this study, a time-series analysis of N-15 translocation at the whole plant level was performed, which indicated that nitrogen recycling among organs was highly dynamic during this period and varied depending on nitrate availability. Leaves collected on vegetative and reproductive nodes were further analyzed by transcriptomics. The data revealed extensive transcriptome changes in leaves of reproductive nodes during early seed development (from flowering to 14 days after flowering), including an up-regulation of genes encoding transporters, and particularly of sulfate that might sustain sulfur metabolism in leaves of the reproductive part. This developmental period was also characterized by a down-regulation of cell wall-associated genes in leaves of both reproductive and vegetative nodes, reflecting a shift in cell wall structure. Later on, 27 days after flowering, genes potentially switching the metabolism of leaves toward senescence were pinpointed, some of which are related to ribosomal RNA processing, autophagy, or transport systems. Transcription factors differentially regulated in leaves between stages were identified and a gene co-expression network pointed out some of them as potential regulators of the above-mentioned biological processes. The same approach was conducted in Medicago truncatula to identify shared regulations with this wild legume species. Altogether the results give a global view of transcriptional events in leaves of legumes at early reproductive stages and provide a valuable resource of candidate genes that could be targeted by reverse genetics to improve nutrient remobilization and/or delay ...

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/31440268; hal-02270632; https://institut-agro-dijon.hal.science/hal-02270632Test; https://institut-agro-dijon.hal.science/hal-02270632/documentTest; https://institut-agro-dijon.hal.science/hal-02270632/file/2019_Gallardo%20et%20al%202019_fpls-10-01014.pdfTest; PUBMED: 31440268; PUBMEDCENTRAL: PMC6693388; WOS: 000479122600001

الإتاحة: https://doi.org/10.3389/fpls.2019.01014Test
https://institut-agro-dijon.hal.science/hal-02270632Test
https://institut-agro-dijon.hal.science/hal-02270632/documentTest
https://institut-agro-dijon.hal.science/hal-02270632/file/2019_Gallardo%20et%20al%202019_fpls-10-01014.pdfTest

المؤلفون: Warakanont, Jaruswan, Li-Beisson, Yonghua, Benning, Christoph

المساهمون: Environnement, Bioénergie, Microalgues et Plantes (EBMP), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Kasetsart University Bangkok, Thailand (KU), Michigan State University East Lansing, Michigan State University System

المصدر: ISSN: 0032-0781.

مصطلحات موضوعية: $Chlamydomonas\ reinhardtii$, lipid catabolism, SUGAR DEPENDENT1, triacylglycerol lipase, N recovery, TAG remobilization, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Degradation of the storage compound triacylglycerol (TAG) is a crucial process in response to environmental stimuli. Failing to respond properly may be detrimental for survival. In microalgae, this process is important for re-growth when conditions become favorable after cells have experienced stresses. Mobilization of TAG is initiated by actions of lipases causing the release of glycerol and free fatty acids, which can be further broken down for energy production or recycled to synthesize membrane lipids. Although key enzymes in the process, TAG lipases remain to be characterized in the model green alga $Chlamydomonas\ reinhardtii$. Here we describe the functional analysis of a putative TAG lipase, i.e. CrLIP4, which shares 44% amino acid identity with the major TAG lipase in $Arabidopsis$ (SUGAR DEPENDENT1- SDP1). The $CrLIP4$ transcript level was down regulated during nitrogen deprivation (ND) when TAG accumulates, but was upregulated during nitrogen recovery (NR) when TAG wasdegraded. Both artificial microRNA knockdown and insertional knockout mutants showed a delay in TAG mobilization during NR. The difference in TAG degradation was more pronounced when the cultures were incubated without acetate in the dark. Furthermore, the $crlip4$ knock-out mutant over-accumulated TAG during optimal growth conditions. Taken together, the results suggest to us that CrLIP4 likely acts as a TAG lipase and plays a major role in TAG homeostasis in $Chlamydomonas$.

العلاقة: cea-02096094; https://cea.hal.science/cea-02096094Test; https://cea.hal.science/cea-02096094/documentTest; https://cea.hal.science/cea-02096094/file/Warakamont-2019-CrLIP4-ms5.pdfTest

الإتاحة: https://doi.org/10.1093/pcp/pcz037Test
https://cea.hal.science/cea-02096094Test
https://cea.hal.science/cea-02096094/documentTest
https://cea.hal.science/cea-02096094/file/Warakamont-2019-CrLIP4-ms5.pdfTest

المؤلفون: Poret, Marine, Chandrasekar, Balakumaran, van Der Hoorn, Renier, Dechaumet, Sylvain, Bouchereau, Alain, Kim, Tae-Hwan, Lee, Bok-Rye, Macquart, Flavien, Hara-Nishimura, Ikuko, Avice, Jean-Christophe

المساهمون: Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Department of economics, Yonsei University, Department of Metabolic Biology, John Innes Centre Norwich, Biotechnology and Biological Sciences Research Council (BBSRC)-Biotechnology and Biological Sciences Research Council (BBSRC), Kyoto University, ANR-11-BTBR-004 RAPSODYN – Investments for the Future: Optimisation of the RAPeSeed Oil content and Yield under low Nitrogen input), a Ph.D. grant from the French Ministry of Research, ERA-IB project “PRODuCE”, the Max Planck Society, COST CM1004 and the University of Oxford, ANR-11-BTBR-0004,RAPSODYN,Optimisation de la teneur et du rendement en huile chez le colza cultivé sous contrainte azotée(2011)

المصدر: ISSN: 1664-462X ; Frontiers in Plant Science ; https://normandie-univ.hal.science/hal-02184367Test ; Frontiers in Plant Science, 2019, 10, pp.46. ⟨10.3389/fpls.2019.00046⟩.

مصطلحات موضوعية: Brassica napus L, nitrogen remobilization efficiency, phytohormones, protease activity, regulation, senescence, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology

الوصف: International audience ; Oilseed rape ( L.) is an oleoproteaginous crop characterized by low N use efficiency (NUE) that is mainly related to a weak Nitrogen Remobilization Efficiency (NRE) during the sequential leaf senescence of the vegetative stages. Based on the hypothesis that proteolysis efficiency is crucial for the improvement of leafNRE, our objective was to characterize key senescence-associated proteolytic mechanisms of two genotypes (Ténor and Samouraï) previously identified with contrasting NREs. To reach this goal, biochemical changes, protease activities and phytohormone patterns were studied in mature leaves undergoing senescence in two genotypes with contrasting NRE cultivated in a greenhouse under limiting or ample nitrate supply. The genotype with the higher NRE (Ténor) possessed enhanced senescence processes in response to nitrate limitation, and this led to greater degradation of soluble proteins compared to the other genotype (Samouraï). This efficient proteolysis is associated with (i) an increase in serine and cysteine protease (CP) activities and (ii) the appearance of new CP activities (RD21-like, SAG12-like, RD19-like, cathepsin-B, XBCP3-like and aleurain-like proteases) during senescence induced by N limitation. Compared to Samouraï, Ténor has a higher hormonal ratio ([salicylic acid] + [abscisic acid])/([cytokinins]) that promotes senescence, particularly under low N conditions, and this is correlated with the stronger protein degradation and serine/CP activities observed during senescence. Short statement: The improvement in N recycling during leaf senescence in a genotype of L. characterized by a high nitrogen remobilization efficiency is related to a high phytohormonal ratio ([salicylic acid] + [abscisic acid])/([cytokinins]) that promotes leaf senescence and is correlated with an increase or the induction of specific serine and cysteine protease activities.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/30778361; hal-02184367; https://normandie-univ.hal.science/hal-02184367Test; https://normandie-univ.hal.science/hal-02184367/documentTest; https://normandie-univ.hal.science/hal-02184367/file/fpls-10-00046_1.pdfTest; PRODINRA: 470601; PUBMED: 30778361; WOS: 000457638300001

الإتاحة: https://doi.org/10.3389/fpls.2019.00046Test
https://normandie-univ.hal.science/hal-02184367Test
https://normandie-univ.hal.science/hal-02184367/documentTest
https://normandie-univ.hal.science/hal-02184367/file/fpls-10-00046_1.pdfTest

المؤلفون: James, Maxence, Masclaux-Daubresse, Céline, Marmagne, Anne, Azzopardi, Marianne, Laine, Philippe, Goux, Didier, Etienne, Philippe, Trouverie, Jacques

المساهمون: Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS (UMR_7086)), Université Paris Diderot - Paris 7 (UPD7)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Interactions Cellules Organismes Environnement (ICORE), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN)

المصدر: ISSN: 1664-462X ; Frontiers in Plant Science ; https://normandie-univ.hal.science/hal-02184364Test ; Frontiers in Plant Science, 2019, 9, ⟨10.3389/fpls.2018.01998⟩.

مصطلحات موضوعية: cysteine protease activity, N remobilization, Reproductive stage, Roots, SAG12, N uptake, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology

الوصف: International audience ; Senescence associated gene (SAG) 12, which encodes a cysteine protease isconsidered to be important in nitrogen (N) allocation to Arabidopsis thaliana seeds.A decrease in the yield and N content of the seeds was observed in the ArabidopsisSAG12 knockout mutants (sag12) relative to the wild type (Col0) under limited nitrogennutrition. However, leaf senescence was similar in both lines. To test whether SAG12is involved in N remobilization from organs other than the leaves, we tested whetherroot N could be used in N mobilization to the seeds. Root architecture, N uptakecapacity and 15N partitioning were compared in the wild type and sag12 under eitherhigh nitrogen (HN) or low nitrogen (LN) conditions. No differences in root architectureor root N uptake capacity were observed between the lines under HN or LN. However,under LN conditions, there was an accumulation of 15N in the sag12 roots comparedto the wild type with lower allocation of 15N to the seeds. This was accompanied by anincrease in root N protein contents and a significant decrease in root cysteine proteaseactivity. SAG12 is expressed in the root stele of the plants at the reproductive stage,particularly under conditions of LN nutrition. Taken together, these results suggest anew role for SAG12. This cysteine protease plays a crucial role in root N remobilizationthat ensures seed filling and sustains yields when nitrogen availability is low.

العلاقة: hal-02184364; https://normandie-univ.hal.science/hal-02184364Test; https://normandie-univ.hal.science/hal-02184364/documentTest; https://normandie-univ.hal.science/hal-02184364/file/fpls-09-01998_1.pdfTest; PRODINRA: 470605; WOS: 000455530600001

الإتاحة: https://doi.org/10.3389/fpls.2018.01998Test
https://normandie-univ.hal.science/hal-02184364Test
https://normandie-univ.hal.science/hal-02184364/documentTest
https://normandie-univ.hal.science/hal-02184364/file/fpls-09-01998_1.pdfTest

المؤلفون: Chen, Qinwu, Shinozaki, Daiki, Luo, Jie, Pottier, Mathieu, Havé, Marien, Marmagne, Anne, Reisdorf-Cren, Michèle, Chardon, Fabien, Thomine, Sébastien, Yoshimoto, Kohki, Masclaux-Daubresse, Céline

المساهمون: Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Meiji University Tokyo, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Approches intégratives du Transport Ionique (MINION), Département Biologie Cellulaire (BioCell), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), ANR-10-LABX-0040,SPS,Saclay Plant Sciences(2010), ANR-12-ADAP-0010,AUTOADAPT,Role de l'AUTOphagie dans l'ADAPTation des plantes aux limitations en nitrate ou suphate dans leur environnement(2012)

المصدر: ISSN: 2073-4409 ; Cells ; https://hal.science/hal-02367568Test ; Cells, 2019, 8 (11), ⟨10.3390/cells8111426⟩.

مصطلحات موضوعية: iron, leaf senescence, nitrogen remobilization, nitrogen use efficiency, plant proteases, zinc, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Nutrient recycling and mobilization from organ to organ all along the plant lifespan is essential for plant survival under changing environments. Nutrient remobilization to the seeds is also essential for good seed production. In this review, we summarize the recent advances made to understand how plants manage nutrient remobilization from senescing organs to sink tissues and what is the contribution of autophagy in this process. Plant engineering manipulating autophagy for better yield and plant tolerance to stresses will be presented.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/31726766; hal-02367568; https://hal.science/hal-02367568Test; https://hal.science/hal-02367568/documentTest; https://hal.science/hal-02367568/file/Chen%20et%20al.%20Cells%202020.pdfTest; PUBMED: 31726766; WOS: 000502266700124

الإتاحة: https://doi.org/10.3390/cells8111426Test
https://hal.science/hal-02367568Test
https://hal.science/hal-02367568/documentTest
https://hal.science/hal-02367568/file/Chen%20et%20al.%20Cells%202020.pdfTest

المؤلفون: Pottier, Mathieu, Dumont, Jean, Masclaux-Daubresse, Céline, Thomine, Sébastien

المساهمون: Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris-Saclay, Approches intégratives du Transport Ionique (MINION), Département Biologie Cellulaire (BioCell), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Département Biochimie, Biophysique et Biologie Structurale (B3S), ANR-16-CE20-0019,ISISTOR,Amélioration du contenu en fer de la graine(2016)

المصدر: ISSN: 0022-0957.

مصطلحات موضوعية: iron loading, iron recycling, leaf senescence, metal, remobilization, 57Fe, premature senescence, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics

الوصف: International audience ; Micronutrient deficiencies affect a large part of the world’s population. These deficiencies are mostly due to the consumption of grains with insufficient content of iron (Fe) or zinc (Zn). Both de novo uptake by roots and recycling from leaves may provide seeds with nutrients. Autophagy, which is a conserved mechanism for nutrient recycling in eukaryotes, was shown to be involved in nitrogen remobilization to seeds. Here, we have investigated the role of this mechanism in micronutrient translocation to seeds. We found that Arabidopsis thaliana plants impaired in autophagy display defects in nutrient remobilization to seeds. In the atg5-1 mutant, which is completely defective in autophagy, the efficiency of Fe translocation from vegetative organs to seeds was severely decreased even when Fe was provided during seed formation. Combining atg5-1 with the sid2 mutation that counteracts premature senescence associated with autophagy deficiency and using 57Fe pulse labeling, we propose a two-step mechanism in which Fe taken up de novo during seed formation is first accumulated in vegetative organs and subsequently remobilized to seeds. Finally, we show that translocation of Zn and manganese (Mn) to seeds is also dependent on autophagy. Fine-tuning autophagy during seed formation opens up new possibilities to improve micronutrient remobilization to seeds

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/30395253; hal-02173634; https://hal.science/hal-02173634Test; https://hal.science/hal-02173634/documentTest; https://hal.science/hal-02173634/file/ery388.pdfTest; PRODINRA: 468557; PUBMED: 30395253; PUBMEDCENTRAL: PMC6363094; WOS: 000459350700013

الإتاحة: https://doi.org/10.1093/jxb/ery388Test
https://hal.science/hal-02173634Test
https://hal.science/hal-02173634/documentTest
https://hal.science/hal-02173634/file/ery388.pdfTest