المؤلفون: Frédéric Delbac, Julie Brunet, Philippe Poirier, Michel Dapoigny, Céline Nourrisson, Julien Scanzi

المساهمون: CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand, Laboratoire Microorganismes : Génome et Environnement (LMGE), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)

المصدر: Frontiers in Microbiology
Frontiers in Microbiology, 2021, 12, ⟨10.3389/fmicb.2021.713347⟩
Frontiers in Microbiology, Vol 12 (2021)
Frontiers in Microbiology, Frontiers Media, 2021, 12, ⟨10.3389/fmicb.2021.713347⟩

مصطلحات موضوعية: Microbiology (medical), Mycobiota, [SDV]Life Sciences [q-bio], Gut flora, Microbiology, 18S ribosomal RNA, 03 medical and health sciences, medicine, Feces, Irritable bowel syndrome, Original Research, 030304 developmental biology, irritable bowel syndrome, 0303 health sciences, Tenericutes, Blastocystis, biology, gut microbiota, 030306 microbiology, 16S/18S ribosomal RNA gene analysis, biology.organism_classification, medicine.disease, eukaryome, QR1-502, 3. Good health, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, IBS-C, Ruminococcaceae

الوصف: Blastocystis is the most frequently isolated protozoan from human stool. Its role in human health is still debated, and a high prevalence was reported in irritable bowel syndrome (IBS) subjects, suggesting a potential link with microbiota. In the present study, we aimed to investigate prokaryotic and eukaryotic microbiota in both IBS-C (constipated) and healthy individuals. We recruited 35 IBS-C patients and 23 healthy subjects, from which 12 and 11 carried Blastocystis, respectively. We performed 16S and 18S rRNA high-throughput sequencing on feces. Whereas we did not observe differences between infected and non-infected controls, several phyla were significantly modified in IBS-C patients according to the presence of Blastocystis. Tenericutes phylum and Ruminococcaceae family were especially increased in Blastocystis carriers. Furthermore, colonization with Blastocystis was associated with discrete changes in the microbial eukaryome, particularly among the Fungi taxa. Depending on the group of patients considered, the mycobiota changes do not go in the same direction and seem more deleterious in the IBS-C group. These results encourage further in vivo and in vitro investigations concerning the role of Blastocystis in the gut environment.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::911aaee4069469a0663b99177cf10700Test
https://hal.science/hal-03349938Test

المؤلفون: Pamela Afouda, Grégory Dubourg, Claudia Andrieu, Magali Richez, Didier Raoult, Enora Tomei, Pierre-Edouard Fournier, Niokhor Dione, Fabrizio Di Pinto, Jean-Christophe Lagier, Sory Ibrahima Traore

المساهمون: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, INSB-INSB-Centre National de la Recherche Scientifique (CNRS), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA)

المصدر: Antonie van Leeuwenhoek
Antonie van Leeuwenhoek, Springer Verlag, 2019, 112 (6), pp.905-918. ⟨10.1007/s10482-018-01223-x⟩
Antonie van Leeuwenhoek, 2019, 112 (6), pp.905-918. ⟨10.1007/s10482-018-01223-x⟩

مصطلحات موضوعية: DNA, Bacterial, Male, 0301 basic medicine, 030106 microbiology, Saudi Arabia, Biology, Microbiology, Feces, Young Adult, 03 medical and health sciences, Human gut, Genome Size, Genus, RNA, Ribosomal, 16S, Humans, Molecular Biology, Phylogeny, ComputingMilieux_MISCELLANEOUS, Base Composition, Clostridiales, Phylogenetic tree, Ruminococcus species, Genomics, General Medicine, Fecal bacteriotherapy, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Gastrointestinal Microbiome, 030104 developmental biology, Culturomics, Optimal growth, Family Ruminococcaceae, Genome, Bacterial

الوصف: Using the culturomics approach, we isolated two strains, Marseille-P2963 and Marseille-P3753, from the intestinal microbiota of a 19-year-old healthy Saudi Arabian Bedouin male and from a 32-year-old healthy Senegalese male faecal transplant donor. Here, we studied their phenotypic, phylogenetic and genomic characteristics. Both strains were phylogenetically related, but different from Ruminococcus species. Bacterial cells were anaerobic, rod-shaped, non-spore-forming and not motile, with neither catalase nor oxidase activities. Their growth temperatures ranged from 28 to 45 °C, with an optimal growth at 37 °C. The genomes are 2,842,720 bp- and 2,707,061 bp-long respectively. The G + C contents are 47.18% and 46.90%, respectively. Based on these characteristics, we propose the creation of a new genus within the family Ruminococcaceae named Massiliimalia gen. nov., that contains the new species Massiliimalia massiliensis gen. nov., sp. nov., and Massiliimalia timonensis gen. nov., sp. nov. Strains Marseille-P2963T (= CSUR P2963 = DSM 106837) and Marseille-P3753T (= CSUR P3753 = CCUG 71632) are their type strains, respectively.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7046ce21782f5a401926dfa9d4c4d14eTest
https://hal-amu.archives-ouvertes.fr/hal-02202099Test

المؤلفون: Christina Skytte, Marlene Danner Dalgaard, Anders Miki Bojesen, Jørgen S. Agerholm, Emma Lorenzen, Karin Erneholm, Anette Blak Grossi, Nicole Gutman, Egle Kudirkiene

المصدر: Veterinary Research
Veterinary Research, BioMed Central, 2015, 46 (1), pp.125. ⟨10.1186/s13567-015-0274-0⟩
Lorenzen, E, Kudirkiene, E, Gutman, N, Grossi, A B, Agerholm, J S, Erneholm, K, Skytte, C, Dalgaard, M D & Bojesen, A M 2015, ' The vaginal microbiome is stable in prepubertal and sexually mature Ellegaard Göttingen Minipigs throughout an estrous cycle ', Veterinary Research, vol. 46, no. 125 . https://doi.org/10.1186/s13567-015-0274-0Test
Lorenzen, E K, Kudirkiene, E, Gutman, N, Grossi, A B, Agerholm, J S, Erneholm, K S, Skytte, C, Dalgaard, M D & Bojesen, A M 2015, ' The vaginal microbiome is stable in prepubertal and sexually mature Ellegaard Göttingen Minipigs throughout an estrous cycle ', Veterinary Research, vol. 46, 125 . https://doi.org/10.1186/s13567-015-0274-0Test

مصطلحات موضوعية: Swine, Firmicutes, [SDV]Life Sciences [q-bio], Physiology, Estrous Cycle, medicine.disease_cause, Microbiology, 03 medical and health sciences, RNA, Ribosomal, 16S, medicine, Animals, Sexual maturity, Sexual Maturation, 030304 developmental biology, Estrous cycle, 0303 health sciences, General Veterinary, biology, 030306 microbiology, Microbiota, Clostridiales, Sequence Analysis, DNA, Paenibacillaceae, biology.organism_classification, veterinary(all), 3. Good health, medicine.anatomical_structure, Vagina, Swine, Miniature, Female, Chlamydia trachomatis, Research Article, Ruminococcaceae

الوصف: Although the pig has been introduced as an advanced animal model of genital tract infections in women, almost no knowledge exists on the porcine vaginal microbiota, especially in barrier-raised Göttingen Minipigs. In women, the vaginal microbiota plays a crucial role for a healthy vaginal environment and the fate of sexually transmitted infections such as Chlamydia trachomatis infections. Therefore, knowledge on the vaginal microbiota is urgently needed for the minipig model. The aim of this study was to characterize the microbiota of the anterior vagina by 16 s rRNA gene sequencing in prepubertal and sexually mature Göttingen Minipigs during an estrous cycle. The dominating phyla in the vaginal microbiota consisted of Firmicutes, Proteobacteria, Actinobacteria, Bacteriodetes and Tenericutes. The most abundant bacterial families were Enterobacteriaceae, unclassified families from Gammaproteobacteria, Clostridiales Family XI Incertae Sedis, Paenibacillaceae, Lactobacillaceae, Ruminococcaceae and Syntrophaceae. We found a higher abundance of Lactobacillaceae in the prepubertal Göttingen Minipigs compared to sexually mature non-pregnant Göttingen Minipigs. However, correlation tests and diversity parameters revealed a very stable vaginal microbiota in the Göttingen Minipigs, both before and after sexual maturity and on different days throughout an estrous cycle. The vaginal microbiota in Göttingen Minipigs was not dominated by lactobacilli, as it is in women and according to our results the minipig vaginal microbiota is very stable, in opposite to women. These differences should be considered when using the minipig as a model of the genital tract in women. Electronic supplementary material The online version of this article (doi:10.1186/s13567-015-0274-0) contains supplementary material, which is available to authorized users.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e1141b2a4d04ff583b7a9f096cd51e5Test
https://hal.archives-ouvertes.fr/hal-01341434Test

المؤلفون: Hanane El oumami, Olivier Berteau, Marion Leclerc, Joël Doré, Karine Gloux, Fabienne Béguet

المساهمون: MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, French Ministry of Research

المصدر: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2011, 108, pp.4539-4546. ⟨10.1073/pnas.1000066107⟩
Proceedings of the National Academy of Sciences of the United States of America (108), 4539-4546. (2011)

مصطلحات موضوعية: intestinal microbiota, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Adaptation, Biological, functional metagenomics, Genome, glycosyl hydrolase, GUT MICROBIOME, RNA, Ribosomal, 16S, Cloning, Molecular, Intestinal Mucosa, Phylogeny, Glucuronidase, Genetics, 0303 health sciences, Multidisciplinary, biology, HYDROLYSIS, CROHNS-DISEASE, Intestines, ESCHERICHIA-COLI, BACTERIA, GEN. NOV, Ruminococcaceae, Firmicutes, Colloquium Papers, Genetic Vectors, Molecular Sequence Data, BIOLOGICAL-ACTIVITIES, Bacterial genome size, METABOLISM, RATS, 03 medical and health sciences, functional core, Humans, Microbiome, Amino Acid Sequence, FECAL MICROBIOTA, Gene, 030304 developmental biology, Gene Library, 030306 microbiology, Lachnospiraceae, Computational Biology, Sequence Analysis, DNA, biology.organism_classification, Metagenomics, Metagenome

الوصف: In the human gastrointestinal tract, bacterial β- D -glucuronidases (BG; E.C. 3.2.1.31) are involved both in xenobiotic metabolism and in some of the beneficial effects of dietary compounds. Despite their biological significance, investigations are hampered by the fact that only a few BGs have so far been studied. A functional metagenomic approach was therefore performed on intestinal metagenomic libraries using chromogenic glucuronides as probes. Using this strategy, 19 positive metagenomic clones were identified but only one exhibited strong β- D -glucuronidase activity when subcloned into an expression vector. The cloned gene encoded a β- D -glucuronidase (called H11G11-BG) that had distant amino acid sequence homologies and an additional C terminus domain compared with known β- D -glucuronidases. Fifteen homologs were identified in public bacterial genome databases (38–57% identity with H11G11-BG) in the Firmicutes phylum. The genomes identified derived from strains from Ruminococcaceae, Lachnospiraceae, and Clostridiaceae. The genetic context diversity, with closely related symporters and gene duplication, argued for functional diversity and contribution to adaptive mechanisms. In contrast to the previously known β- D -glucuronidases, this previously undescribed type was present in the published microbiome of each healthy adult/child investigated ( n = 11) and was specific to the human gut ecosystem. In conclusion, our functional metagenomic approach revealed a class of BGs that may be part of a functional core specifically evolved to adapt to the human gut environment with major health implications. We propose consensus motifs for this unique Firmicutes β- D -glucuronidase subfamily and for the glycosyl hydrolase family 2.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b90f4583ca2e5eca544820dce530460bTest
https://hal.archives-ouvertes.fr/hal-01001013/documentTest