Cripto is essential to capture mouse epiblast stem cell and human embryonic stem cell pluripotency
| العنوان: | Cripto is essential to capture mouse epiblast stem cell and human embryonic stem cell pluripotency |
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| المؤلفون: | Ann Zeuner, Emilia Pascale, Federica Francescangeli, Annalisa Fico, Mauro Biffoni, Cristina D'Aniello, Gabriella Minchiotti, Claudia Angelini, Dario Acampora, Francesco Russo, Eduardo J. Patriarca, Cecilia Bassalert, Gennaro Andolfi, Claire Chazaud, Alessandro Fiorenzano |
| المساهمون: | Institute of Genetics and Biophysics 'A. Buzzati Traverso', Consiglio Nazionale delle Ricerche [Roma] (CNR), Génétique, Reproduction et Développement (GReD), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), Istituto per le Applicazioni del Calcolo 'Mauro Picone' (IAC), AIRC (11599 to G.M. and 15749 to A.Z.), European Community’s Seventh Framework Programme in the project ENDOSTEM, Epigenomics Flagship Project MIUR-CNR (to G.M. and C.A.), Italian Ministry of Education-University-Research (PRIN and PON Cluster IRMI) to G.M. E.J.P. is supported by Medical Research in Italy (RBNE08HM7T-003). C.C. is supported by ANR (PrEpiSpec) and ARC (PJA 20131200380) grants., National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Nature Communications Nature Communications, Nature Publishing Group, 2016, 7, pp.12589. ⟨10.1038/ncomms12589⟩ Nature Communications, 2016, 7, pp.12589. ⟨10.1038/ncomms12589⟩ Nature communications 7 (2016): 12589. doi:10.1038/ncomms12589 info:cnr-pdr/source/autori:Fiorenzano, Alessandro; Pascale, Emilia; D'Aniello, Cristina; Acampora, Dario; Bassalert, Cecilia; Russo, Francesco; Andolfi, Gennaro; Biffoni, Mauro; Francescangeli, Federica; Zeuner, Ann; Angelini, Claudia; Chazaud, Claire; Patriarca, Eduardo J; Fico, Annalisa; Minchiotti, Gabriella/titolo:Cripto is essential to capture mouse epiblast stem cell and human embryonic stem cell pluripotency./doi:10.1038%2Fncomms12589/rivista:Nature communications/anno:2016/pagina_da:12589/pagina_a:/intervallo_pagine:12589/volume:7 Nature Communications, Vol 7, Iss 1, Pp 1-16 (2016) |
| بيانات النشر: | HAL CCSD, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Pluripotent Stem Cells, Embryonic stem cells, Nodal Protein, Science, General Physics and Astronomy, Embryonic Development, Smad2 Protein, Biology, CRIPTO, Cripto, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, Mice, Animals, Humans, Transcription factor, beta Catenin, reproductive and urinary physiology, Genetics, Mice, Knockout, Cell lineage, Multidisciplinary, Membrane Glycoproteins, Epidermal Growth Factor, urogenital system, Transdifferentiation, Wnt signaling pathway, General Chemistry, Cellular Reprogramming, Embryonic stem cell, Cell biology, Neoplasm Proteins, Wnt Proteins, 030104 developmental biology, [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis, Epiblast, embryonic structures, biological phenomena, cell phenomena, and immunity, Stem cell, NODAL, Germ Layers, hormones, hormone substitutes, and hormone antagonists |
| الوصف: | Known molecular determinants of developmental plasticity are mainly transcription factors, while the extrinsic regulation of this process has been largely unexplored. Here we identify Cripto as one of the earliest epiblast markers and a key extracellular determinant of the naive and primed pluripotent states. We demonstrate that Cripto sustains mouse embryonic stem cell (ESC) self-renewal by modulating Wnt/β-catenin, whereas it maintains mouse epiblast stem cell (EpiSC) and human ESC pluripotency through Nodal/Smad2. Moreover, we provide unprecedented evidence that Cripto controls the metabolic reprogramming in ESCs to EpiSC transition. Remarkably, Cripto deficiency attenuates ESC lineage restriction in vitro and in vivo, and permits ESC transdifferentiation into trophectoderm lineage, suggesting that Cripto has earlier functions than previously recognized. All together, our studies provide novel insights into the current model of mammalian pluripotency and contribute to the understanding of the extrinsic regulation of the first cell lineage decision in the embryo. Stem cell plasticity is crucial for early embryo development and the differentiation of stem cells. Here, the authors show that the extracellular protein Cripto sustains mouse ESC self-renewal and maintains mouse EpiSC as well as human ESC pluripotency and controls the metabolic reprogramming in ESCs to EpiSC transition. |
| اللغة: | English |
| تدمد: | 2041-1723 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73660b7c32c23c7dd4853ebe74282868Test https://hal.archives-ouvertes.fr/hal-01923147/file/ncomms12589.pdfTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....73660b7c32c23c7dd4853ebe74282868 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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