Vascular imaging with contrast agent in hard and soft tissues using microcomputed-tomography
| العنوان: | Vascular imaging with contrast agent in hard and soft tissues using microcomputed-tomography |
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| المؤلفون: | Pauline Bléry, Yves Amouriq, Paul Pilet, Arnaud Vanden-Bossche, Jérôme Guicheux, Nicole Mathieu, Audrey Thery, Pierre Weiss, Florent Espitalier |
| المساهمون: | Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Nantes - UFR Odontologie, Université de Nantes (UN), Biologie intégrative du tissu osseux, Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d’ORL et de chirurgie cervico-faciale [CHU Hôtel Dieu, Nantes], Centre hospitalier universitaire de Nantes (CHU Nantes), Laboratoire de recherche en régénération des tissus sains irradiés [Fontenay-aux-Roses] (LR2I), Institut de Radioprotection et de Sûreté Nucléaire - IRSN [Fontenay-aux-Roses], Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de recherche en régénération des tissus sains irradiés (IRSN/PRP-HOM/SRBE/LR2I), Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Jehan, Frederic |
| المصدر: | Journal of Microscopy Journal of Microscopy, Wiley, 2016, 262, pp.40-49. ⟨10.1111/jmi.12339⟩ Journal of Microscopy, 2016, 262, pp.40-49. ⟨10.1111/jmi.12339⟩ |
| بيانات النشر: | HAL CCSD, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Pathology, medicine.medical_specialty, Histology, Materials science, Radiography, Contrast Media, Barium sulphate, micro-CT, Pathology and Forensic Medicine, Rats, Sprague-Dawley, 03 medical and health sciences, vascularization, In vivo, medicine, Animals, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Vascular imaging, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, medicine.diagnostic_test, business.industry, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Angiography, MESH: Barium sulphate, contrast agent, imaging, micro-CT, vascularization, Soft tissue, imaging, X-Ray Microtomography, Microcomputed tomography, contrast agent, 030104 developmental biology, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Blood Vessels, Barium Sulfate, business, Ex vivo, Biomedical engineering |
| الوصف: | International audience; Vascularization is essential for many tissues and is a main requisite for various tissue-engineering strategies. Different techniques are used for highlighting vasculature, in vivo and ex vivo, in 2-D or 3-D including histological staining, immunohistochemistry, radiography, angiography, microscopy, computed tomography (CT) or micro-CT, both stand-alone andsynchrotron system. Vascularization can be studied with or without a contrast agent. This paper presents the resultsobtained with the latest Skyscan micro-CT (Skyscan 1272, Bruker, Belgium) following barium sulphate injection replacingthe bloodstream in comparison with results obtained with a Skyscan In Vivo 1076. Different hard and soft tissues wereperfused with contrast agent and were harvested. Samples were analysed using both forms of micro-CT, and improvedresultswere shown using this new micro-CT. This study highlights the vasculature using micro-CT methods. The resultsobtained with the Skyscan 1272 are clearly defined compared to results obtained with Skyscan 1076. In particular, this instrument highlights the high number of small vessels, which were not seen before at lower resolution. This new micro-CTopens broader possibilities in detection and characterization of the 3-D vascular tree to assess vascular tissue engineeringstrategies. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0022-2720 1365-2818 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8ae59e5212b3c0cfdc1d2224350d026Test https://www.hal.inserm.fr/inserm-01564012/documentTest |
| حقوق: | EMBARGO |
| رقم الانضمام: | edsair.doi.dedup.....a8ae59e5212b3c0cfdc1d2224350d026 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00222720 13652818 |
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