المؤلفون: Girondé, Alexandra, Etienne, Philippe, Trouverie, Jacques, Bouchereau, Alain, Le Cahérec, Françoise, Leport, Laurent, Orsel, Mathilde, Niogret, Marie-Françoise, Nesi, Nathalie, Soulay, Fabienne, Masclaux-Daubresse, Céline, Avice, Jean-Christophe

المساهمون: Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, ANR-11-BTBR-0004,RAPSODYN,Optimisation de la teneur et du rendement en huile chez le colza cultivé sous contrainte azotée(2011)

المصدر: ISSN: 1471-2229 ; BMC Plant Biology ; https://hal.science/hal-01392652Test ; BMC Plant Biology, 2015, 15 (1), pp.59. ⟨10.1186/s12870-015-0437-1⟩ ; http://www.biomedcentral.com/1471-2229/15/59Test.

مصطلحات موضوعية: Brassica napus, Leaf senescence, N remobilization efficiency, N use efficiency, Proteolysis, Proteasome, [SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology

الوصف: International audience ; Oilseed rape is the third largest oleaginous crop in the world but requires high levels of N fertilizer of which only 50% is recovered in seeds. This weak N use efficiency is associated with a low foliar N remobilization, leading to a significant return of N to the soil and a risk of pollution. Contrary to what is observed during senescence in the vegetative stages, N remobilization from stems and leaves is considered efficient during monocarpic senescence. However, the contribution of stems towards N management and the cellular mechanisms involved in foliar remobilization remain largely unknown. To reach this goal, the N fluxes at the whole plant level from bolting to mature seeds and the processes involved in leaf N remobilization and proteolysis were investigated in two contrasting genotypes (Aviso and Oase) cultivated under ample or restricted nitrate supply. Results: During seed filling in both N conditions, Oase efficiently allocated the N from uptake to seeds while Aviso favoured a better N remobilization from stems and leaves towards seeds. Nitrate restriction decreased seed yield and oil quality for both genotypes but Aviso had the best seed N filling. Under N limitation, Aviso had a better N remobilization from leaves to stems before the onset of seed filling. Afterwards, the higher N remobilization from stems and leaves of Aviso led to a higher final N amount in seeds. This high leaf N remobilization is associated with a better degradation/export of insoluble proteins, oligopeptides, nitrate and/or ammonia. By using an original method based on the determination of Rubisco degradation in the presence of inhibitors of proteases, efficient proteolysis associated with cysteine proteases and proteasome activities was identified as the mechanism of N remobilization. Conclusion: The results confirm the importance of foliar N remobilization after bolting to satisfy seed filling and highlight that an efficient proteolysis is mainly associated with (i) cysteine proteases and proteasome ...

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/25848818; hal-01392652; https://hal.science/hal-01392652Test; https://hal.science/hal-01392652/documentTest; https://hal.science/hal-01392652/file/The%20contrasting%20N%20management.pdfTest; OKINA: ua9621; PRODINRA: 305892; PUBMED: 25848818; WOS: 000352193800001

الإتاحة: https://doi.org/10.1186/s12870-015-0437-1Test
https://hal.science/hal-01392652Test
https://hal.science/hal-01392652/documentTest
https://hal.science/hal-01392652/file/The%20contrasting%20N%20management.pdfTest

المؤلفون: Avila Ospina, Liliana, Moison, Michaël, Yoshimoto, Koki, Masclaux-Daubresse, Celine

المساهمون: Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, CETIOM (Centre Technique des Oleagineux), Biologie et Amelioration des Plantes, INRA, INRA Package programme, European Project: 264394

المصدر: ISSN: 0022-0957.

مصطلحات موضوعية: Nitrogen remobilization, Rubisco containing bodies, plant cell death, proteolysis, vesicular trafficking, [SDV]Life Sciences [q-bio]

الوصف: Large numbers of publications have appeared over the last few years, dealing with the molecular details of the regulation and process of the autophagy machinery in animals, plants, and unicellular eukaryotic organisms. This strong interest is caused by the fact that the autophagic process is involved in the adaptation of organisms to their environment and to stressful conditions, thereby contributing to cell and organism survival and longevity. In plants, as in other eukaryotes, autophagy is associated with longevity as mutants display early and strong leaf senescence symptoms, however, the exact role of autophagy as a pro-survival or pro-death process is unclear. Recently, evidence that autophagy participates in nitrogen remobilization has been provided, but the duality of the role of autophagy in leaf longevity and/or nutrient recycling through cell component catabolism remains. This review aims to give an overview of leaf senescence-associated processes from the physiological point of view and to discuss relationships between nutrient recycling, proteolysis, and autophagy. The dual role of autophagy as a pro-survival or pro-death process is discussed.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/24687977; info:eu-repo/grantAgreement//264394/EU/Enhanced plant productivity through control of lifespan/; hal-01204073; https://hal.science/hal-01204073Test; https://hal.science/hal-01204073/documentTest; https://hal.science/hal-01204073/file/2014_Avila-Ospina_Journal%20of%20Experimental%20Botany_1.pdfTest; PRODINRA: 275386; PUBMED: 24687977; WOS: 000339954000003

الإتاحة: https://doi.org/10.1093/jxb/eru039Test
https://hal.science/hal-01204073Test
https://hal.science/hal-01204073/documentTest
https://hal.science/hal-01204073/file/2014_Avila-Ospina_Journal%20of%20Experimental%20Botany_1.pdfTest

المؤلفون: Gironde, Alexandra, Poret, Marine, Etienne, Philippe, Trouverie, Jacques, Bouchereau, Alain, Le Cahérec, Françoise, Leport, Laurent, Niogret, Françoise, Avice, Jean-Christophe

المساهمون: Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, ANR-11-BTBR-0004,RAPSODYN,Optimisation de la teneur et du rendement en huile chez le colza cultivé sous contrainte azotée(2011)

المصدر: ISSN: 2223-7747 ; Plants ; https://normandie-univ.hal.science/hal-02183519Test ; Plants, 2016, 5 (1), pp.1. ⟨10.3390/plants5010001⟩.

مصطلحات موضوعية: Brassica napus, N remobilization, acidic proteases, genotypic variability, proteolysis, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology

الوصف: International audience

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/27135221; hal-02183519; https://normandie-univ.hal.science/hal-02183519Test; PUBMED: 27135221; PUBMEDCENTRAL: PMC4844419

الإتاحة: https://doi.org/10.3390/plants5010001Test
https://normandie-univ.hal.science/hal-02183519Test

المؤلفون: Anne Guiboileau, Jérémy Lothier, Marianne Azzopardi, Kohki Yoshimoto, Fabienne Soulay, Céline Masclaux-Daubresse, Anne Marmagne, Liliana Avila-Ospina

المساهمون: Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA)

المصدر: New Phytologist
New Phytologist, Wiley, 2013, 199 (3), pp.683-694
New Phytologist, Wiley, 2013, 199 (3), pp.683-694. ⟨10.1111/nph.12307⟩

مصطلحات موضوعية: 0106 biological sciences, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Physiology, leaf senescence, Arabidopsis, Plant Science, 01 natural sciences, AMINO-ACIDS, Biomass, 2. Zero hunger, 0303 health sciences, aminopeptidase, biology, medicine.diagnostic_test, PROFILING APPROACH, nitrogen remobilization, REMOBILIZATION, carboxypeptidase, Biochemistry, 5-BISPHOSPHATE CARBOXYLASE OXYGENASE, Carbohydrate Metabolism, RNA Interference, RIBULOSE-1, EUGLENA-GRACILIS, nitrate availability, Nitrogen, Proteolysis, Blotting, Western, Protein degradation, 03 medical and health sciences, Ribosomal protein, Glutamate-Ammonia Ligase, GLUTAMINE-SYNTHETASE, Glutamine synthetase, medicine, Autophagy, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, PLANTS, 030304 developmental biology, selective autophagy, SENESCING LEAVES, Nitrates, Arabidopsis Proteins, Glutamate dehydrogenase, RuBisCO, DEGRADATION, biology.organism_classification, Carbon, Plant Leaves, Mutation, biology.protein, 010606 plant biology & botany, Peptide Hydrolases

الوصف: Article de revue (Article scientifique dans une revue à comité de lecture); International audience; Autophagy is present at a basal level in all plant tissues and is induced during leaf ageing and in response to nitrogen (N) starvation. Nitrogen remobilization from the rosette to the seeds is impaired in autophagy mutants. This report focuses on the role of autophagy in leaf N management and proteolysis during plant ageing.Metabolites, enzyme activities and protein contents were monitored in several autophagy-defective (atg) Arabidopsis mutants grown under low and high nitrate conditions.Results showed that carbon (C) and N statuses were affected in atg mutants before any senescence symptoms appeared. atg mutants accumulated larger amounts of ammonium, amino acids and proteins than wild type, and were depleted in sugars. Over-accumulation of proteins in atg mutants was selective and occurred despite higher endopeptidase and carboxypeptidase activities. Specific over-accumulation of the ribosomal proteins S6 and L13 subunits, and of catalase and glutamate dehydrogenase proteins was observed. atg mutants also accumulated peptides putatively identified as degradation products of the Rubisco large subunit and glutamine synthetase 2 (GS2). Incomplete chloroplast protein degradation resulting from autophagy defects could explain the higher N concentrations measured in atg rosettes and defects in N remobilization.It is concluded that autophagy controls C:N status and protein content in leaves of Arabidopsis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05d76d3c8ab4056b0979362c4d8290acTest
https://hal.archives-ouvertes.fr/hal-01168786Test

المؤلفون: Alexandra, Girondé, Philippe, Etienne, Jacques, Trouverie, Alain, Bouchereau, Françoise, Le Cahérec, Laurent, Leport, Mathilde, Orsel, Marie-Françoise, Niogret, Nathalie, Nesi, Deleu, Carole, Fabienne, Soulay, Céline, Masclaux-Daubresse, Jean-Christophe, Avice

المساهمون: Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST

المصدر: BMC Plant Biology
BMC Plant Biology, BioMed Central, 2015, 15 (1), pp.59. ⟨10.1186/s12870-015-0437-1⟩
BMC Plant Biology, 2015, 15 (1), pp.59. ⟨10.1186/s12870-015-0437-1⟩

مصطلحات موضوعية: Chlorophyll, Genotype, Nitrogen, Ribulose-Bisphosphate Carboxylase, [SDV]Life Sciences [q-bio], Leaf senescence, Glutamate Dehydrogenase, Glutamate-Ammonia Ligase, Plant Oils, N use efficiency, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Protease Inhibitors, Biomass, Amino Acids, ComputingMilieux_MISCELLANEOUS, Nitrates, Plant Stems, Proteasome, Brassica napus, food and beverages, Plant Leaves, Kinetics, Solubility, Seeds, Proteolysis, N remobilization efficiency, Research Article

الوصف: Background Oilseed rape is the third largest oleaginous crop in the world but requires high levels of N fertilizer of which only 50% is recovered in seeds. This weak N use efficiency is associated with a low foliar N remobilization, leading to a significant return of N to the soil and a risk of pollution. Contrary to what is observed during senescence in the vegetative stages, N remobilization from stems and leaves is considered efficient during monocarpic senescence. However, the contribution of stems towards N management and the cellular mechanisms involved in foliar remobilization remain largely unknown. To reach this goal, the N fluxes at the whole plant level from bolting to mature seeds and the processes involved in leaf N remobilization and proteolysis were investigated in two contrasting genotypes (Aviso and Oase) cultivated under ample or restricted nitrate supply. Results During seed filling in both N conditions, Oase efficiently allocated the N from uptake to seeds while Aviso favoured a better N remobilization from stems and leaves towards seeds. Nitrate restriction decreased seed yield and oil quality for both genotypes but Aviso had the best seed N filling. Under N limitation, Aviso had a better N remobilization from leaves to stems before the onset of seed filling. Afterwards, the higher N remobilization from stems and leaves of Aviso led to a higher final N amount in seeds. This high leaf N remobilization is associated with a better degradation/export of insoluble proteins, oligopeptides, nitrate and/or ammonia. By using an original method based on the determination of Rubisco degradation in the presence of inhibitors of proteases, efficient proteolysis associated with cysteine proteases and proteasome activities was identified as the mechanism of N remobilization. Conclusion The results confirm the importance of foliar N remobilization after bolting to satisfy seed filling and highlight that an efficient proteolysis is mainly associated with (i) cysteine proteases and proteasome activities and (ii) a fine coordination between proteolysis and export mechanisms. In addition, the stem may act as transient storage organs in the case of an asynchronism between leaf N remobilization and N demand for seed filling. Electronic supplementary material The online version of this article (doi:10.1186/s12870-015-0437-1) contains supplementary material, which is available to authorized users.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::d5ef697b88a78c71f47a9e2e5787d200Test
https://hal.archives-ouvertes.fr/hal-01392652/documentTest

المؤلفون: Avice, Jean-Christophe, Etienne, Philippe

المساهمون: Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA)

المصدر: ISSN: 0022-0957.

مصطلحات موضوعية: Brassica napus, leaf senescence, N remobilization efficiency, N use efficiency, proteolysis, N transport, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences

الوصف: International audience ; Despite its worldwide economic importance for food (oil, meal) and non-food (green energy and chemistry) uses, oilseed rape has a low nitrogen (N) use efficiency (NUE), mainly due to the low N remobilization efficiency (NRE) observed during the vegetative phase when sequential leaf senescence occurs. Assuming that improvement of NRE is the main lever for NUE optimization, unravelling the cellular mechanisms responsible for the recycling of proteins (the main N source in leaf) during sequential senescence is a prerequisite for identifying the physiological and molecular determinants that are associated with high NRE. The development of a relevant molecular indicator (SAG12/Cab) of leaf senescence progression in combination with a N-15-labelling method were used to decipher the N remobilization associated with sequential senescence and to determine modulation of this process by abiotic factors especially N deficiency. Interestingly, in young leaves, N starvation delayed senescence and induced BnD22, a water-soluble chlorophyll-binding protein that acts against oxidative alterations of chlorophylls and exhibits a protease inhibitor activity. Through its dual function, BnD22 may help to sustain sink growth of stressed plants and contribute to a better utilization of N recycled from senescent leaves, a physiological trait that could improve NUE. Proteomics approaches have revealed that proteolysis involves chloroplastic FtsH protease in the early stages of senescence, aspartic protease during the course of leaf senescence, and the proteasome beta 1 subunit, mitochondria processing protease and SAG12 (cysteine protease) during the later senescence phases. Overall, the results constitute interesting pathways for screening genotypes with high NRE and NUE.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/24790115; hal-02633666; https://hal.inrae.fr/hal-02633666Test; PRODINRA: 286785; PUBMED: 24790115; WOS: 000339954000004

الإتاحة: https://doi.org/10.1093/jxb/eru177Test
https://hal.inrae.fr/hal-02633666Test

المؤلفون: Philippe Etienne, Jean Christophe Avice

المساهمون: Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA)

المصدر: Journal of Experimental Botany
Journal of Experimental Botany, Oxford University Press (OUP), 2014, 65 (14), pp.3813-3824. ⟨10.1093/jxb/eru177⟩

مصطلحات موضوعية: 0106 biological sciences, Senescence, proteolysis, Physiology, Nitrogen, leaf senescence, Proteolysis, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Brassica, Plant Science, Oxidative phosphorylation, 7. Clean energy, 01 natural sciences, Models, Biological, 03 medical and health sciences, medicine, N use efficiency, 030304 developmental biology, Plant Proteins, 2. Zero hunger, Abiotic component, 0303 health sciences, Protease, medicine.diagnostic_test, biology, Brassica napus, biology.organism_classification, Cysteine protease, Cell biology, Plant Leaves, Proteasome, [SDE]Environmental Sciences, N remobilization efficiency, N transport, 010606 plant biology & botany

الوصف: International audience; Despite its worldwide economic importance for food (oil, meal) and non-food (green energy and chemistry) uses, oilseed rape has a low nitrogen (N) use efficiency (NUE), mainly due to the low N remobilization efficiency (NRE) observed during the vegetative phase when sequential leaf senescence occurs. Assuming that improvement of NRE is the main lever for NUE optimization, unravelling the cellular mechanisms responsible for the recycling of proteins (the main N source in leaf) during sequential senescence is a prerequisite for identifying the physiological and molecular determinants that are associated with high NRE. The development of a relevant molecular indicator (SAG12/Cab) of leaf senescence progression in combination with a N-15-labelling method were used to decipher the N remobilization associated with sequential senescence and to determine modulation of this process by abiotic factors especially N deficiency. Interestingly, in young leaves, N starvation delayed senescence and induced BnD22, a water-soluble chlorophyll-binding protein that acts against oxidative alterations of chlorophylls and exhibits a protease inhibitor activity. Through its dual function, BnD22 may help to sustain sink growth of stressed plants and contribute to a better utilization of N recycled from senescent leaves, a physiological trait that could improve NUE. Proteomics approaches have revealed that proteolysis involves chloroplastic FtsH protease in the early stages of senescence, aspartic protease during the course of leaf senescence, and the proteasome beta 1 subunit, mitochondria processing protease and SAG12 (cysteine protease) during the later senescence phases. Overall, the results constitute interesting pathways for screening genotypes with high NRE and NUE.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a81e507b46cf27cdc6871fe020c8e1d7Test
https://hal.inrae.fr/hal-02633666Test