The vaginal microbiome is stable in prepubertal and sexually mature Ellegaard Göttingen Minipigs throughout an estrous cycle
| العنوان: | The vaginal microbiome is stable in prepubertal and sexually mature Ellegaard Göttingen Minipigs throughout an estrous cycle |
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| المؤلفون: | Christina Skytte, Marlene Danner Dalgaard, Anders Miki Bojesen, Jørgen S. Agerholm, Emma Lorenzen, Karin Erneholm, Anette Blak Grossi, Nicole Gutman, Egle Kudirkiene |
| المصدر: | Veterinary Research Veterinary Research, BioMed Central, 2015, 46 (1), pp.125. ⟨10.1186/s13567-015-0274-0⟩ Lorenzen, E, Kudirkiene, E, Gutman, N, Grossi, A B, Agerholm, J S, Erneholm, K, Skytte, C, Dalgaard, M D & Bojesen, A M 2015, ' The vaginal microbiome is stable in prepubertal and sexually mature Ellegaard Göttingen Minipigs throughout an estrous cycle ', Veterinary Research, vol. 46, no. 125 . https://doi.org/10.1186/s13567-015-0274-0Test Lorenzen, E K, Kudirkiene, E, Gutman, N, Grossi, A B, Agerholm, J S, Erneholm, K S, Skytte, C, Dalgaard, M D & Bojesen, A M 2015, ' The vaginal microbiome is stable in prepubertal and sexually mature Ellegaard Göttingen Minipigs throughout an estrous cycle ', Veterinary Research, vol. 46, 125 . https://doi.org/10.1186/s13567-015-0274-0Test |
| بيانات النشر: | HAL CCSD, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Swine, Firmicutes, [SDV]Life Sciences [q-bio], Physiology, Estrous Cycle, medicine.disease_cause, Microbiology, 03 medical and health sciences, RNA, Ribosomal, 16S, medicine, Animals, Sexual maturity, Sexual Maturation, 030304 developmental biology, Estrous cycle, 0303 health sciences, General Veterinary, biology, 030306 microbiology, Microbiota, Clostridiales, Sequence Analysis, DNA, Paenibacillaceae, biology.organism_classification, veterinary(all), 3. Good health, medicine.anatomical_structure, Vagina, Swine, Miniature, Female, Chlamydia trachomatis, Research Article, Ruminococcaceae |
| الوصف: | Although the pig has been introduced as an advanced animal model of genital tract infections in women, almost no knowledge exists on the porcine vaginal microbiota, especially in barrier-raised Göttingen Minipigs. In women, the vaginal microbiota plays a crucial role for a healthy vaginal environment and the fate of sexually transmitted infections such as Chlamydia trachomatis infections. Therefore, knowledge on the vaginal microbiota is urgently needed for the minipig model. The aim of this study was to characterize the microbiota of the anterior vagina by 16 s rRNA gene sequencing in prepubertal and sexually mature Göttingen Minipigs during an estrous cycle. The dominating phyla in the vaginal microbiota consisted of Firmicutes, Proteobacteria, Actinobacteria, Bacteriodetes and Tenericutes. The most abundant bacterial families were Enterobacteriaceae, unclassified families from Gammaproteobacteria, Clostridiales Family XI Incertae Sedis, Paenibacillaceae, Lactobacillaceae, Ruminococcaceae and Syntrophaceae. We found a higher abundance of Lactobacillaceae in the prepubertal Göttingen Minipigs compared to sexually mature non-pregnant Göttingen Minipigs. However, correlation tests and diversity parameters revealed a very stable vaginal microbiota in the Göttingen Minipigs, both before and after sexual maturity and on different days throughout an estrous cycle. The vaginal microbiota in Göttingen Minipigs was not dominated by lactobacilli, as it is in women and according to our results the minipig vaginal microbiota is very stable, in opposite to women. These differences should be considered when using the minipig as a model of the genital tract in women. Electronic supplementary material The online version of this article (doi:10.1186/s13567-015-0274-0) contains supplementary material, which is available to authorized users. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0928-4249 1297-9716 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e1141b2a4d04ff583b7a9f096cd51e5Test https://hal.archives-ouvertes.fr/hal-01341434Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4e1141b2a4d04ff583b7a9f096cd51e5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09284249 12979716 |
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